LOCUS       BBa_K1813014            1342 bp    DNA     linear       17-May-2024
DEFINITION  <i>nicC</i> Expression Cassette
ACCESSION   BBa_K1813014
VERSION     BBa_K1813014.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     CDS             88..1239
                     /label=BBa_K1813006
     stem_loop       1255..1274
                     /label=T7 TE
     misc_feature    1281..1281
                     /label=stop
     polyA_site      1275..1288
                     /label=polya
     misc_feature    1248..1288
                     /label=BBa_B0012
     terminator      1248..1288
                     /label=BBa_B0012
     stem_loop       1309..1331
                     /label=stem_loop
     misc_feature    1297..1342
                     /label=BBa_B0011
     terminator      1297..1342
                     /label=BBa_B0011
     terminator      1248..1342
                     /label=BBa_B0014
     terminator      1248..1342
                     /label=BBa_K823017
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg cgcggtcgcc agaagatcgc aattgttggt
      121 gctggcttag gcggcgcagc cgctgctact ttattgcagc aagctggttt tgatgttgaa
      181 gtgtttgagc aggccccagc attcacccgc ttaggcgctg gcatccacat cggccctaac
      241 gttatgaaga tttttcgccg tatgggttta gagcagaaat tagagttaat gggctctcac
      301 ccagacttct ggttttcacg tgacggtaac accggtgact acttgtcacg cattccatta
      361 ggcgagttcg cccgccgcga gtatggcgct gcctacatta ccatccaccg cggcgattta
      421 cacgcattgc agattgaagc aattcagcca ggtaccgttc actttggcaa acgtttagaa
      481 aaaattgttg atgaaggtga ccaggttcgt ttagacttcg ctgacggtac ccacaccgtg
      541 gcagacattg tgatcggcgc agacggcatt cattcaaaga ttcgcgaaga gttattgggt
      601 gccgaagccc caatctattc tggttgggtg gctcatcgtg cattgattcg tggtgtgaat
      661 ttagcccaac acgccgatgt tttcgagcca tgcgttaagt ggtggagtga agaccgtcat
      721 atgatggtgt attatactac aggcaagcgt gatgaatatt atttcgttac cggcgtgcct
      781 cacgaagcct gggattttca aggcgcattc gtggacagtt ctcaggaaga gatgcgcgca
      841 gccttcgaag gttaccaccc aacagtgcaa aaattgatcg acgctaccga atcaatcaca
      901 aaatggcctt tacgcaaccg caatccttta cctttgtggt cacgtggccg cttagtttta
      961 ttgggcgacg cttgtcatcc tatgaaacct catatggcac agggcgcttg catggctatt
     1021 gaggatgcag caatgttaac ccgttgttta caggaaactg gcttgagtga tcaccgcaca
     1081 gctttcgcat tatacgaagc aaaccgcaag gaacgcgcct cacaggttca gagtgtttct
     1141 aatgccaaca cttggttata ttctcaggaa gatcctgcct gggtatatgg ttatgacttg
     1201 tatggtcagc agttagaaag tggcgaagct gcttaataat actagagtca cactggctca
     1261 ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa gccagattat
     1321 taatccggct tttttattat tt
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