LOCUS       BBa_K1813015            1000 bp    DNA     linear       22-Nov-2024
DEFINITION  <i>nicD</i> Expression Casette
ACCESSION   BBa_K1813015
VERSION     BBa_K1813015.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     CDS             88..897
                     /label=BBa_K1813007
     stem_loop       913..932
                     /label=T7 TE
     misc_feature    939..939
                     /label=stop
     polyA_site      933..946
                     /label=polya
     misc_feature    906..946
                     /label=BBa_B0012
     terminator      906..946
                     /label=BBa_B0012
     stem_loop       967..989
                     /label=stem_loop
     misc_feature    955..1000
                     /label=BBa_B0011
     terminator      955..1000
                     /label=BBa_B0011
     terminator      906..1000
                     /label=BBa_B0014
     terminator      906..1000
                     /label=BBa_K823017
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg agtacctttg tggcaggcgg caacgtatct
      121 gccaacggca ttcgtcaaca ctatttgcgt tatggcggca aaggtcacgc tttaatctta
      181 gttccaggca ttacatctcc tgccatcaca tggggcttcg tggctgaacg cttaggtcat
      241 tactttgaca cctatgtatt ggacgttcgt ggtcgtggct tatctagttc aggccctgac
      301 ttggattacg gcactgacgc atgcgccgcc gacatcccag cttttgccgc agccttgggt
      361 ttggatagtt accacttatt aggtcattct atgggcgccc gttttgcaat ccgcgccgcc
      421 gcacaaggcg ccccaggctt gcagcgctta gtgttagtag accctccagt aagtggtcca
      481 ggccgtcgtg catacccatc aaaattacct tggtacgtgg attctattcg ccaagctacc
      541 gtaggcatga gtggtgacga tatgcgcgcc ttctgtgcta cttggtcaga tgaacagtta
      601 gctttacgcg cagagtggtt acacacatgt tatgaaccag ccatcgtgcg tgcatttgac
      661 gattttcacg aagttgatat tcaccagtat ttacctgctg tgcgccagcc tgccttatta
      721 atggttgccg gtcgtggcgg cgttattgaa ccacgtgata ttgccgaaat gcgtgagttg
      781 aaaccagaca tccaagtggc ctacgttgat aatgccggcc acatgatccc atgggatgat
      841 ttagacggtt tcttcgccgc attcggcgat ttcttagatc acccattggt ttaataatac
      901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa
      961 tataaaaagc cagattatta atccggcttt tttattattt
//