LOCUS       BBa_K1813016             946 bp    DNA     linear       17-May-2024
DEFINITION  <i>nicE</i> Expression Casette
ACCESSION   BBa_K1813016
VERSION     BBa_K1813016.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     CDS             88..843
                     /label=BBa_K1813008
     stem_loop       859..878
                     /label=T7 TE
     misc_feature    885..885
                     /label=stop
     polyA_site      879..892
                     /label=polya
     misc_feature    852..892
                     /label=BBa_B0012
     terminator      852..892
                     /label=BBa_B0012
     stem_loop       913..935
                     /label=stem_loop
     misc_feature    901..946
                     /label=BBa_B0011
     terminator      901..946
                     /label=BBa_B0011
     terminator      852..946
                     /label=BBa_B0014
     terminator      852..946
                     /label=BBa_K823017
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg acccagttat atcgtatcgg tcagattgta
      121 cctagttcta ataccacaat ggagaccgaa attcctgcta tgttgaatgc ccgtcaggct
      181 atccgccctg aacgttttac ttttcactca tctcgcatgc gcatgaaaca agtaaaaaaa
      241 gaagaattag ctgctatgga tgcagagagt gaccgctgcg cagttgaatt gtctgatgca
      301 aaagtggatg tattaggtta tgcatgctta gtagcaatta tggcaatggg cttaggctac
      361 catcgccagt cagagaaacg cttacagcaa gcaaccgccg ataacgatgc attggcccct
      421 gtgatcactt cagcaggcgc cttagtggaa gcattacatg tgatgaaggc caaacgcatc
      481 gccattgtag caccatatat gaagccattg accgaattag tggttaacta catccgtgaa
      541 gaaggcttcg aggttcagga ctggcgtgca ttagaaatcc ctgataattt ggccgtagcc
      601 cgccatgatc cagctaactt gcctggtatt gtggcaggta tggatttgga aggtgttgat
      661 gtagttgttt tatctgcatg tgtgcaaatg cagtcattac ctgccgtggc caaggttgaa
      721 gcccaaaccg gtaaacctgt tgtgacagcc gccatcgcaa ccacctacgc catgttgaaa
      781 gccttagatt tagagcctat tgtacctggc gcaggcgctt tgttatctgg tgcttactaa
      841 taatactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
      901 agagaatata aaaagccaga ttattaatcc ggctttttta ttattt
//