LOCUS       BBa_K1813017             835 bp    DNA     linear       17-May-2024
DEFINITION  <i>nicF</i> Expression Cassette
ACCESSION   BBa_K1813017
VERSION     BBa_K1813017.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     CDS             88..732
                     /label=BBa_K1813009
     stem_loop       748..767
                     /label=T7 TE
     misc_feature    774..774
                     /label=stop
     polyA_site      768..781
                     /label=polya
     misc_feature    741..781
                     /label=BBa_B0012
     terminator      741..781
                     /label=BBa_B0012
     stem_loop       802..824
                     /label=stem_loop
     misc_feature    790..835
                     /label=BBa_B0011
     terminator      790..835
                     /label=BBa_B0011
     terminator      741..835
                     /label=BBa_B0014
     terminator      741..835
                     /label=BBa_K823017
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg agtgatgctc aatcagcacg tgataattat
      121 cagggcgtgt ggggccagcg tatcggtttt ggccgtaaac cagcattatt aatgatcgac
      181 ttcatgcagg gctacacaac ccctggcgca cctttatacg ctcctggtgt agttgcagca
      241 gtggagcaag cagcaggttt gttggctttg gcccgcgact gtggcacatt agtggtgcat
      301 acaaacattc gctatcagcc tccacatttc gccgatggtg gtgtgtgggt tcgtaaggcc
      361 cctgtgatga aagacatggt tgaaggcaac cctttagctg ccttctgtga agccgtagca
      421 cctcaggcag gcgaagtagt attgtctaaa cagtatgcaa gtgccttttt tgccacttca
      481 ttggccccat tattacatgc ccagggtgtt gatactgtgg tattggccgg ctgtagtacc
      541 tctggttgca ttcgtgcaag tgctgtggat gctatgcagc acggcttccg cacaatcgta
      601 gtgcgcgaat gcgtaggtga ccgccatagt gacccacatg aagcaaactt attcgatatt
      661 gacagtaaat atggcgatgt ggttacccgt caagatgcta tgcagcagtt acgccactta
      721 gcaggctaat aatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      781 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tattt
//