LOCUS       BBa_K1813018            2233 bp    DNA     linear       17-May-2024
DEFINITION  AgambNADPH Expression Cassette
ACCESSION   BBa_K1813018
VERSION     BBa_K1813018.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     CDS             88..2130
                     /label=BBa_K1813010
     stem_loop       2146..2165
                     /label=T7 TE
     misc_feature    2172..2172
                     /label=stop
     polyA_site      2166..2179
                     /label=polya
     misc_feature    2139..2179
                     /label=BBa_B0012
     terminator      2139..2179
                     /label=BBa_B0012
     stem_loop       2200..2222
                     /label=stem_loop
     misc_feature    2188..2233
                     /label=BBa_B0011
     terminator      2188..2233
                     /label=BBa_B0011
     terminator      2139..2233
                     /label=BBa_B0014
     terminator      2139..2233
                     /label=BBa_K823017
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg gacgctcaga ccgaaaccga agtgccagca
      121 ggctctgtga gtgatgaacc tttcttaggt cctttagaca tcgttttatt ggtatcttta
      181 ttggccggta cagcatggta tttgttgaaa ggcaagaaaa aagaaagtca ggcatcacag
      241 tttaaatcat atagtattca accaacaacc gtgaatacaa tgaccatggt ggaaaacagt
      301 ttcatcaaga aattacagag ttctggtcgt cgcttagttg ttttttatgg ttcacagacc
      361 ggtacagcag aagaatttgc cggtcgctta gctaaagaag gcatccgtta tcagatgaaa
      421 ggtatggtag cagatccaga agaatgtaac atggaagagt tgttaatgtt aaaagacatc
      481 gacaagtcat tggctgtgtt ttgcttagca acctatggtg aaggtgatcc aaccgataac
      541 tgtatggaat tttatgattg gatccagaac aatgatttag atatgactgg cttgaattac
      601 gctgtgtttg gtttgggtaa taaaacctac gagcattata ataaagttgg catttacgtt
      661 gataaacgct tggaagaatt gggtgcaaac cgtgtttttg aattgggctt gggcgatgac
      721 gatgccaaca ttgaagatta tttcatcacc tggaaagaaa agttttggcc aacagtttgc
      781 gattatttcg gtattgaaag tacaggcgaa gacgttttga tgcgtcagta tcgcttattg
      841 gagcaaccag atgtgtcagc cgatcgcatt tacaccggcg aggtggcacg cttacacagt
      901 ttgcaaaccc aacgcccacc ttttgacgcc aaaaaccctt tcttggctcc aatcaaagtt
      961 aatcgtgagt tgcataaagc aggtggccgt agttgcatgc atgtggaatt tgatatcgaa
     1021 ggtagtaaaa tgcgttatga ggctggtgac catttagcca tgtatcctgt taacgatcgc
     1081 gatttggttg agcgtttagg tcgtttatgt aacgctgaat tggacaccgt tttctcttta
     1141 atcaacacag acacagattc ttcaaaaaaa catccattcc cttgcccaac cacttaccgt
     1201 actgccttaa cccactactt agaaatcaca gccttgccac gtacccacat tttgaaagaa
     1261 ttagctgaat attgcggtga ggaaaaagat aaagaatttt tgcgttttat ctcttctacc
     1321 gcaccagatg gcaaggcaaa gtatcaggag tgggttcagg atagttgtcg caacatcgta
     1381 cacgtgttgg aagacattcc ttcttgccac cctcctattg accacgtgtg tgagttatta
     1441 ccacgtttac aacctcgcta ccactctatt tcttctagtt caaaattaca ccctaccaca
     1501 gttcatgtga ccgcagtatt agtaaaatat gaaaccaaaa ccggtcgctt aaataaaggt
     1561 gtagctacca ctttcttggc cgagaaacat ccaaatgatg gcgaaccagc cccacgtgtt
     1621 ccaattttta tccgcaaatc tcagtttcgt ttgcctccaa aaccagaaac cccagtgatt
     1681 atggtgggcc caggtaccgg tttggctcct ttccgtggtt ttattcagga gcgcgaccat
     1741 tgcaaacagg agggcaaaga aattggtcag accactttat attttggctg tcgcaaacgt
     1801 agtgaagact atatctatga agacgaattg gaggactaca gtaaacgcgg catcatcaat
     1861 ttacgtgtgg ccttttcacg cgatcaagag aaaaaagtgt atgtgactca cttattagaa
     1921 caggactctg atttgatttg gagtgtgatc ggtgaaaata agggtcattt ctatatttgc
     1981 ggtgacgcca aaaacatggc tactgatgtg cgcaacatct tgttgaaagt aattcgtagt
     2041 aaaggcggct tatcagagac cgaagctcaa caatacatca agaagatgga ggcacagaag
     2101 cgttattctg ctgatgtatg gtcataataa tactagagtc acactggctc accttcgggt
     2161 gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc
     2221 ttttttatta ttt
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