LOCUS BBa_K1813022 2273 bp DNA linear 06-Dec-2025 DEFINITION nicE Expression Cassette with LacI Reversed ACCESSION BBa_K1813022 VERSION BBa_K1813022.1 FEATURES Location/Qualifiers promoter 1..1319 /label=BBa_K1813019 promoter 1328..1367 /label=Ptac promoter 1334..1339 /label=-35 promoter 1356..1360 /label=-10 misc_feature 1363..1388 /label=lacO1 site promoter 1328..1388 /label=BBa_K864400 misc_feature 1401..1404 /label=conserved RBS 1397..1408 /label=BBa_B0034 CDS 1415..2170 /label=BBa_K1813008 stem_loop 2186..2205 /label=T7 TE misc_feature 2212..2212 /label=stop polyA_site 2206..2219 /label=polya misc_feature 2179..2219 /label=BBa_B0012 terminator 2179..2219 /label=BBa_B0012 stem_loop 2240..2262 /label=stem_loop misc_feature 2228..2273 /label=BBa_B0011 terminator 2228..2273 /label=BBa_B0011 terminator 2179..2273 /label=BBa_B0014 terminator 2179..2273 /label=BBa_K823017 misc_feature 1328..2273 /label=BBa_K1813016 ORIGIN 1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa 61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac 121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga 301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg 421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg 481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg 541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc 601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc 661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat 721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg 781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc 841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc 901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac 961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac 1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc 1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt 1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa 1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag 1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat 1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga 1381 taacaattta ctagagaaag aggagaaata ctagatgacc cagttatatc gtatcggtca 1441 gattgtacct agttctaata ccacaatgga gaccgaaatt cctgctatgt tgaatgcccg 1501 tcaggctatc cgccctgaac gttttacttt tcactcatct cgcatgcgca tgaaacaagt 1561 aaaaaaagaa gaattagctg ctatggatgc agagagtgac cgctgcgcag ttgaattgtc 1621 tgatgcaaaa gtggatgtat taggttatgc atgcttagta gcaattatgg caatgggctt 1681 aggctaccat cgccagtcag agaaacgctt acagcaagca accgccgata acgatgcatt 1741 ggcccctgtg atcacttcag caggcgcctt agtggaagca ttacatgtga tgaaggccaa 1801 acgcatcgcc attgtagcac catatatgaa gccattgacc gaattagtgg ttaactacat 1861 ccgtgaagaa ggcttcgagg ttcaggactg gcgtgcatta gaaatccctg ataatttggc 1921 cgtagcccgc catgatccag ctaacttgcc tggtattgtg gcaggtatgg atttggaagg 1981 tgttgatgta gttgttttat ctgcatgtgt gcaaatgcag tcattacctg ccgtggccaa 2041 ggttgaagcc caaaccggta aacctgttgt gacagccgcc atcgcaacca cctacgccat 2101 gttgaaagcc ttagatttag agcctattgt acctggcgca ggcgctttgt tatctggtgc 2161 ttactaataa tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 2221 actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta ttt //