LOCUS       BBa_K1813023            2162 bp    DNA     linear       17-May-2024
DEFINITION  <i>nicF</i> Expression Cassette with LacI Reversed
ACCESSION   BBa_K1813023
VERSION     BBa_K1813023.1  
FEATURES             Location/Qualifiers
     promoter        1..1319
                     /label=BBa_K1813019
     promoter        1328..1367
                     /label=Ptac
     promoter        1334..1339
                     /label=-35
     promoter        1356..1360
                     /label=-10
     misc_feature    1363..1388
                     /label=lacO1 site
     promoter        1328..1388
                     /label=BBa_K864400
     misc_feature    1401..1404
                     /label=conserved
     RBS             1397..1408
                     /label=BBa_B0034
     CDS             1415..2059
                     /label=BBa_K1813009
     stem_loop       2075..2094
                     /label=T7 TE
     misc_feature    2101..2101
                     /label=stop
     polyA_site      2095..2108
                     /label=polya
     misc_feature    2068..2108
                     /label=BBa_B0012
     terminator      2068..2108
                     /label=BBa_B0012
     stem_loop       2129..2151
                     /label=stem_loop
     misc_feature    2117..2162
                     /label=BBa_B0011
     terminator      2117..2162
                     /label=BBa_B0011
     terminator      2068..2162
                     /label=BBa_B0014
     terminator      2068..2162
                     /label=BBa_K823017
     misc_feature    1328..2162
                     /label=BBa_K1813017
ORIGIN
        1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa
       61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac
      121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
      301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
      361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
      421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
      481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
      541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
      601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
      661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
      721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
      781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
      841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
      901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
      961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
     1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
     1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
     1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
     1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag
     1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat
     1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga
     1381 taacaattta ctagagaaag aggagaaata ctagatgagt gatgctcaat cagcacgtga
     1441 taattatcag ggcgtgtggg gccagcgtat cggttttggc cgtaaaccag cattattaat
     1501 gatcgacttc atgcagggct acacaacccc tggcgcacct ttatacgctc ctggtgtagt
     1561 tgcagcagtg gagcaagcag caggtttgtt ggctttggcc cgcgactgtg gcacattagt
     1621 ggtgcataca aacattcgct atcagcctcc acatttcgcc gatggtggtg tgtgggttcg
     1681 taaggcccct gtgatgaaag acatggttga aggcaaccct ttagctgcct tctgtgaagc
     1741 cgtagcacct caggcaggcg aagtagtatt gtctaaacag tatgcaagtg ccttttttgc
     1801 cacttcattg gccccattat tacatgccca gggtgttgat actgtggtat tggccggctg
     1861 tagtacctct ggttgcattc gtgcaagtgc tgtggatgct atgcagcacg gcttccgcac
     1921 aatcgtagtg cgcgaatgcg taggtgaccg ccatagtgac ccacatgaag caaacttatt
     1981 cgatattgac agtaaatatg gcgatgtggt tacccgtcaa gatgctatgc agcagttacg
     2041 ccacttagca ggctaataat actagagtca cactggctca ccttcgggtg ggcctttctg
     2101 cgtttatata ctagagagag aatataaaaa gccagattat taatccggct tttttattat
     2161 tt
//