LOCUS BBa_K1813024 3014 bp DNA linear 22-Dec-2024 DEFINITION CYP2D6 Expression Cassette with LacI Reversed ACCESSION BBa_K1813024 VERSION BBa_K1813024.1 FEATURES Location/Qualifiers promoter 1..1319 /label=BBa_K1813019 promoter 1328..1367 /label=Ptac promoter 1334..1339 /label=-35 promoter 1356..1360 /label=-10 misc_feature 1363..1388 /label=lacO1 site promoter 1328..1388 /label=BBa_K864400 misc_feature 1401..1404 /label=conserved RBS 1397..1408 /label=BBa_B0034 CDS 1415..2911 /label=BBa_K1813005 stem_loop 2927..2946 /label=T7 TE misc_feature 2953..2953 /label=stop polyA_site 2947..2960 /label=polya misc_feature 2920..2960 /label=BBa_B0012 terminator 2920..2960 /label=BBa_B0012 stem_loop 2981..3003 /label=stem_loop misc_feature 2969..3014 /label=BBa_B0011 terminator 2969..3014 /label=BBa_B0011 terminator 2920..3014 /label=BBa_B0014 terminator 2920..3014 /label=BBa_K823017 misc_feature 1328..3014 /label=BBa_K1813013 ORIGIN 1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa 61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac 121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga 301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg 421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg 481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg 541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc 601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc 661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat 721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg 781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc 841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc 901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac 961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac 1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc 1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt 1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa 1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag 1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat 1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga 1381 taacaattta ctagagaaag aggagaaata ctagatgggc ttagaagcat tagttccatt 1441 agcagtgatc gtggcaattt ttttattgtt ggtggattta atgcatcgcc gtcagcgctg 1501 ggcagcccgt tatccacctg gccctttgcc attgcctggt ttaggcaact tattacatgt 1561 ggactttcaa aacactcctt actgttttga tcaattacgt cgtcgctttg gtgacgtatt 1621 ctcattacaa ttggcatgga ccccagttgt tgtgttgaat ggcttggcag ctgtgcgcga 1681 ggctttagtt acccacggcg aggataccgc cgaccgtcct ccagttccta tcactcagat 1741 cttgggcttt ggccctcgta gtcaaggcgt tttcttagca cgctacggcc cagcttggcg 1801 tgaacagcgc cgctttagtg tgtctacctt acgtaatttg ggtttgggta aaaaatcatt 1861 ggagcagtgg gttaccgagg aagccgcttg cttgtgcgcc gcctttgcca accactcagg 1921 tcgtcctttc cgccctaatg gtttgttgga taaagctgtg tcaaacgtga tcgcatcatt 1981 gacatgcggt cgccgttttg agtacgatga tcctcgcttt ttacgtttgt tggatttagc 2041 acaggaaggt ttaaaggaag aatctggttt tttgcgtgag gtgttaaacg ccgtgcctgt 2101 gttgttacac attcctgcat tagcaggtaa agtattacgc ttccaaaagg ctttcttgac 2161 acaattagat gaattattga ctgaacaccg catgacctgg gacccagcac agccaccacg 2221 cgatttaacc gaagcttttt tggccgaaat ggaaaaagct aaaggcaacc cagaatcatc 2281 tttcaatgac gaaaacttac gtattgtagt tgcagattta ttttctgccg gcatggtaac 2341 cacttcaacc actttggcct ggggcttgtt attgatgatc ttgcaccctg acgtgcaacg 2401 tcgtgttcaa caggagatcg atgacgttat cggtcaggtt cgtcgtccag agatgggtga 2461 tcaggcacat atgccttata caactgctgt gatccatgaa gtacagcgtt ttggcgatat 2521 tgtgccttta ggcatgaccc atatgaccag tcgcgatatt gaggtgcaag gtttccgcat 2581 tccaaagggc accacattga ttaccaactt atcatctgtt ttgaaagatg aagccgtgtg 2641 ggaaaaacca ttccgtttcc atcctgagca ttttttggat gcccaaggtc atttcgttaa 2701 accagaagca tttttacctt tttcagcagg tcgtcgtgca tgcttaggtg agcctttggc 2761 acgtatggag ttattcttat tctttaccag tttgttacag cattttagtt ttagtgtgcc 2821 taccggccag ccacgtccta gtcaccatgg tgtattcgcc tttttggttt caccaagtcc 2881 atatgaattg tgcgcagttc cacgctaata atactagagt cacactggct caccttcggg 2941 tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg 3001 cttttttatt attt //