LOCUS BBa_K1813026 2915 bp DNA linear 22-Dec-2024 DEFINITION cch2 Expression Cassette with LacI Reversed ACCESSION BBa_K1813026 VERSION BBa_K1813026.1 FEATURES Location/Qualifiers promoter 1..1319 /label=BBa_K1813019 promoter 1328..1367 /label=Ptac promoter 1334..1339 /label=-35 promoter 1356..1360 /label=-10 misc_feature 1363..1388 /label=lacO1 site promoter 1328..1388 /label=BBa_K864400 misc_feature 1401..1404 /label=conserved RBS 1397..1408 /label=BBa_B0034 CDS 1415..2812 /label=BBa_K1813000 stem_loop 2828..2847 /label=T7 TE misc_feature 2854..2854 /label=stop polyA_site 2848..2861 /label=polya misc_feature 2821..2861 /label=BBa_B0012 terminator 2821..2861 /label=BBa_B0012 stem_loop 2882..2904 /label=stem_loop misc_feature 2870..2915 /label=BBa_B0011 terminator 2870..2915 /label=BBa_B0011 terminator 2821..2915 /label=BBa_B0014 terminator 2821..2915 /label=BBa_K823017 misc_feature 1328..2915 /label=BBa_K1813001 ORIGIN 1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa 61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac 121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga 301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg 421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg 481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg 541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc 601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc 661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat 721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg 781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc 841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc 901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac 961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac 1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc 1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt 1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa 1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag 1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat 1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga 1381 taacaattta ctagagaaag aggagaaata ctagatgtca ttgattgcaa ttaagaacgc 1441 tgactggatc ttaaccatgg atggcgcccg tcgcgtaatc gcagacggct ctattttaat 1501 cgataaagac cgtattcgcg ccatcggtaa aagtgctgtg atcgagattc ctccagagac 1561 aaccaaagtg attgacggtc gcggcaaagt ggttttgcca ggcttaattg acagtcatac 1621 ccaccactca ttacacttag gccgtggctt ggctgacgaa tgtgacattc agacattctt 1681 atatcgtcgc ttgtacccaa tcgaagccag tttaaatgac gaggatgcct atatctcagc 1741 attattatgc cagttagaaa tgattaaaag tggtaccacc tctatcatcg atgctggcaa 1801 ctactttcca gaggctacct tacgtgcatt tggcacaaca ggtatgcgtg gcgttgttgc 1861 acgttcaaca tttgacattc ctacttcatc tttgggttct ttacctgctc aggtgtttgc 1921 agaggagaca aatgtagcct taaaacgtgc cgaagaattt gtagagcgta actctggcgc 1981 atgcgacggc cgtgtgcagg catggttaca gttgcgcgtt ttacctaatt gtagtgatga 2041 gttgtgccgt ggtttgaaat ctattgctga ccgtttgggc gttcgctatg aagcacacgc 2101 cgcctttacc aaagaagtgt atgaagctag taaattacag ttcggcaaga gtgaagtgcg 2161 tcgtttggat gatttaggta tcttaggcga aggtttatta ttagctcata tgggttggtt 2221 aaccccacgc gatattttgt tattgattag ttcaaaaact aatgttgtat tatgcccaac 2281 ctctagtgta caccaggcta tgggttctat cgccttcggc cacgtgccag aattattaga 2341 aatgggtgtg aacgttgcat taggtactga tggtggtcct cacggcacaa atgacttggt 2401 acgccagatt ttcgtggccg caggcggcta taaagaagtt cgcttggatg ctacaatcat 2461 gcctccagaa acagttttag aaatggcaac cgttaatggt gcacgcgcca tgggtatgtc 2521 tgaccaggta ggctcaatcg agccaggtaa aaaagccgac attacaatct tcgactctcg 2581 ccgccctgaa tggcgcccat tacataatcc tgttgcaaat ttagtatatt gcgcaaacgg 2641 taattcagca gataccgtga ttgtagacgg ccgcatttta atggaaaacc gtaaaatctt 2701 aacctttaac gaggatgacg tgattaccga agcacagcgt cgctcagttg aaatcggtgc 2761 tcgtgctggc ttgttggaat acggtcgccc acgctggcca gttcattaat aatactagag 2821 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa 2881 aaagccagat tattaatccg gcttttttat tattt //