LOCUS BBa_K1813027 3083 bp DNA linear 25-Nov-2024 DEFINITION CPY6CM1 Expression Cassette with LacI Reversed ACCESSION BBa_K1813027 VERSION BBa_K1813027.1 FEATURES Location/Qualifiers promoter 1..1319 /label=BBa_K1813019 promoter 1328..1367 /label=Ptac promoter 1334..1339 /label=-35 promoter 1356..1360 /label=-10 misc_feature 1363..1388 /label=lacO1 site promoter 1328..1388 /label=BBa_K864400 misc_feature 1401..1404 /label=conserved RBS 1397..1408 /label=BBa_B0034 CDS 1415..2980 /label=BBa_K1813003 stem_loop 2996..3015 /label=T7 TE misc_feature 3022..3022 /label=stop polyA_site 3016..3029 /label=polya misc_feature 2989..3029 /label=BBa_B0012 terminator 2989..3029 /label=BBa_B0012 stem_loop 3050..3072 /label=stem_loop misc_feature 3038..3083 /label=BBa_B0011 terminator 3038..3083 /label=BBa_B0011 terminator 2989..3083 /label=BBa_B0014 terminator 2989..3083 /label=BBa_K823017 misc_feature 1328..3083 /label=BBa_K1813011 ORIGIN 1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa 61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac 121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga 301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg 421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg 481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg 541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc 601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc 661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat 721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg 781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc 841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc 901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac 961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac 1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc 1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt 1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa 1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag 1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat 1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga 1381 taacaattta ctagagaaag aggagaaata ctagatggaa ttattagaaa tcgtaaagtc 1441 agccatggat acacattctg tattattatt cttatcagtg atggtatatt tgttatacgt 1501 gtatcgcgat aagtttcatt actggtcaaa acgtggcgtt ccttgccaaa gtccagcaca 1561 gtcaattgtt cgtaccttcc gtttagtatt gcgcatggat tcttttaccg acaacttcta 1621 tcgtgtgtat aaggcattcg acggtcatcc ttatgtgggt tctttagagt taaccaaacc 1681 aatcttagta gtgcgcgatc ctgaattggc acgcattgtg ttagttaaat cttttagtag 1741 tttttctggc cgtttaaaat ctccagacac aacattggac ccattaagta atcatttatt 1801 cactttaaac ggtgaaaaat ggcgccaggt acgtcacaaa actgccaccg ctttttctac 1861 cgccaagtta aaaaacatgt ttcacagttt gaaggattgt gctcgtgaaa tggatgccta 1921 tatggaacgc gcaattggtg ataaaggcga tgtggaattt gatgctttta aagtgatgtc 1981 taattatact ttagaagtta tcggcgcttg tgctatgggc atcaaatgcg attctatcca 2041 tgacgaagaa accgaattta aacgtttaag tcgcgatttc tttcgttttg acgctcgccg 2101 catgatcttc acattgttgg atttattaca ccctaaattg cctgttttat tgaaatggaa 2161 agcagtacgc cctgaggtag aaaacttctt ccgcgaagcc attaaagaag cagcttcttt 2221 gaaagagtca gaagcagccg ctcgcaccga ttttttacag attttaatcg attttcagaa 2281 atctgaaaaa gcttcaaaaa ccgatgctgg taacgacact gaattggtgt ttaccgataa 2341 catcattggt ggcgttatcg gttctttctt ttcagctggc tatgagccta ccgctgctgc 2401 tttgactttt tgtttgtatg aattagctcg tcaccctcag gttcaggcca aattacatga 2461 aggcatctta gcagtaaagg aaaagttggg cgatgatatc gaatatgaaa acttaaaaga 2521 gtttaaatat gcaaatcaag taatcgatga aactttgcgt ttgtaccctg ccagtggtat 2581 tttggtgcgt acttgtacag agccttttaa attaccagat tctgacgttg tgattgaaaa 2641 aggcacaaaa gtgttcgtgt catcatatgg cttacagacc gatcctcgct actttcctga 2701 gcctgagaaa tttgatccag aacgcttctc agaagaaaat aaagagaaaa ttttgccagg 2761 tacctactta actttcggtg atggccctcg cttatgcatt gcaatgcgtt tagctttgat 2821 ggacgtgaaa atgatgatgg ttcgtttggt gtcaaaatat gaaattcata ccaccccaaa 2881 gaccccaaaa aaaattacct ttgacactaa ctcatttact gtgcagccag ccgaaaaagt 2941 gtggttacgt tttcgccgcc gcgcaagtac cccttaataa tactagagtc acactggctc 3001 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta 3061 ttaatccggc ttttttatta ttt //