LOCUS BBa_K1813028 3560 bp DNA linear 22-Dec-2024 DEFINITION LacI Reversed AgamNADPH Reductase ACCESSION BBa_K1813028 VERSION BBa_K1813028.1 FEATURES Location/Qualifiers promoter 1..1319 /label=BBa_K1813019 promoter 1328..1367 /label=Ptac promoter 1334..1339 /label=-35 promoter 1356..1360 /label=-10 misc_feature 1363..1388 /label=lacO1 site promoter 1328..1388 /label=BBa_K864400 misc_feature 1401..1404 /label=conserved RBS 1397..1408 /label=BBa_B0034 CDS 1415..3457 /label=BBa_K1813010 stem_loop 3473..3492 /label=T7 TE misc_feature 3499..3499 /label=stop polyA_site 3493..3506 /label=polya misc_feature 3466..3506 /label=BBa_B0012 terminator 3466..3506 /label=BBa_B0012 stem_loop 3527..3549 /label=stem_loop misc_feature 3515..3560 /label=BBa_B0011 terminator 3515..3560 /label=BBa_B0011 terminator 3466..3560 /label=BBa_B0014 terminator 3466..3560 /label=BBa_K823017 misc_feature 1328..3560 /label=BBa_K1813018 ORIGIN 1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa 61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac 121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga 301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg 421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg 481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg 541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc 601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc 661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat 721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg 781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc 841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc 901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac 961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac 1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc 1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt 1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa 1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag 1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat 1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga 1381 taacaattta ctagagaaag aggagaaata ctagatggac gctcagaccg aaaccgaagt 1441 gccagcaggc tctgtgagtg atgaaccttt cttaggtcct ttagacatcg ttttattggt 1501 atctttattg gccggtacag catggtattt gttgaaaggc aagaaaaaag aaagtcaggc 1561 atcacagttt aaatcatata gtattcaacc aacaaccgtg aatacaatga ccatggtgga 1621 aaacagtttc atcaagaaat tacagagttc tggtcgtcgc ttagttgttt tttatggttc 1681 acagaccggt acagcagaag aatttgccgg tcgcttagct aaagaaggca tccgttatca 1741 gatgaaaggt atggtagcag atccagaaga atgtaacatg gaagagttgt taatgttaaa 1801 agacatcgac aagtcattgg ctgtgttttg cttagcaacc tatggtgaag gtgatccaac 1861 cgataactgt atggaatttt atgattggat ccagaacaat gatttagata tgactggctt 1921 gaattacgct gtgtttggtt tgggtaataa aacctacgag cattataata aagttggcat 1981 ttacgttgat aaacgcttgg aagaattggg tgcaaaccgt gtttttgaat tgggcttggg 2041 cgatgacgat gccaacattg aagattattt catcacctgg aaagaaaagt tttggccaac 2101 agtttgcgat tatttcggta ttgaaagtac aggcgaagac gttttgatgc gtcagtatcg 2161 cttattggag caaccagatg tgtcagccga tcgcatttac accggcgagg tggcacgctt 2221 acacagtttg caaacccaac gcccaccttt tgacgccaaa aaccctttct tggctccaat 2281 caaagttaat cgtgagttgc ataaagcagg tggccgtagt tgcatgcatg tggaatttga 2341 tatcgaaggt agtaaaatgc gttatgaggc tggtgaccat ttagccatgt atcctgttaa 2401 cgatcgcgat ttggttgagc gtttaggtcg tttatgtaac gctgaattgg acaccgtttt 2461 ctctttaatc aacacagaca cagattcttc aaaaaaacat ccattccctt gcccaaccac 2521 ttaccgtact gccttaaccc actacttaga aatcacagcc ttgccacgta cccacatttt 2581 gaaagaatta gctgaatatt gcggtgagga aaaagataaa gaatttttgc gttttatctc 2641 ttctaccgca ccagatggca aggcaaagta tcaggagtgg gttcaggata gttgtcgcaa 2701 catcgtacac gtgttggaag acattccttc ttgccaccct cctattgacc acgtgtgtga 2761 gttattacca cgtttacaac ctcgctacca ctctatttct tctagttcaa aattacaccc 2821 taccacagtt catgtgaccg cagtattagt aaaatatgaa accaaaaccg gtcgcttaaa 2881 taaaggtgta gctaccactt tcttggccga gaaacatcca aatgatggcg aaccagcccc 2941 acgtgttcca atttttatcc gcaaatctca gtttcgtttg cctccaaaac cagaaacccc 3001 agtgattatg gtgggcccag gtaccggttt ggctcctttc cgtggtttta ttcaggagcg 3061 cgaccattgc aaacaggagg gcaaagaaat tggtcagacc actttatatt ttggctgtcg 3121 caaacgtagt gaagactata tctatgaaga cgaattggag gactacagta aacgcggcat 3181 catcaattta cgtgtggcct tttcacgcga tcaagagaaa aaagtgtatg tgactcactt 3241 attagaacag gactctgatt tgatttggag tgtgatcggt gaaaataagg gtcatttcta 3301 tatttgcggt gacgccaaaa acatggctac tgatgtgcgc aacatcttgt tgaaagtaat 3361 tcgtagtaaa ggcggcttat cagagaccga agctcaacaa tacatcaaga agatggaggc 3421 acagaagcgt tattctgctg atgtatggtc ataataatac tagagtcaca ctggctcacc 3481 ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta 3541 atccggcttt tttattattt //