LOCUS BBa_K1813031 2573 bp DNA linear 13-Mar-2025 DEFINITION nicX Expression Cassette with LacI Reversed ACCESSION BBa_K1813031 VERSION BBa_K1813031.1 FEATURES Location/Qualifiers promoter 1..1319 /label=BBa_K1813019 promoter 1328..1367 /label=Ptac promoter 1334..1339 /label=-35 promoter 1356..1360 /label=-10 misc_feature 1363..1388 /label=lacO1 site promoter 1328..1388 /label=BBa_K864400 misc_feature 1401..1404 /label=conserved RBS 1397..1408 /label=BBa_B0034 CDS 1415..2470 /label=BBa_K1813029 stem_loop 2486..2505 /label=T7 TE misc_feature 2512..2512 /label=stop polyA_site 2506..2519 /label=polya misc_feature 2479..2519 /label=BBa_B0012 terminator 2479..2519 /label=BBa_B0012 stem_loop 2540..2562 /label=stem_loop misc_feature 2528..2573 /label=BBa_B0011 terminator 2528..2573 /label=BBa_B0011 terminator 2479..2573 /label=BBa_B0014 terminator 2479..2573 /label=BBa_K823017 misc_feature 1328..2573 /label=BBa_K1813030 ORIGIN 1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa 61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac 121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga 301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg 421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg 481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg 541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc 601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc 661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat 721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg 781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc 841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc 901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac 961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac 1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc 1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt 1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa 1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag 1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat 1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga 1381 taacaattta ctagagaaag aggagaaata ctagatgcca gtaagtaatg cacagttaac 1441 ccagatgttc gaacacgtgt taaaattaag tcgcgtggat gaaactcagt ctgttgcagt 1501 gttaaaaagt cattatagtg accctcgcac tgtgaacgca gctatggagg cagcacaacg 1561 tttgaaagct aaggtttatg ccgttgaatt acctgcattt aaccatccaa ccgctatggg 1621 taatgatatg accgcctatt gcggcgatac cgcattaact ggtaacttag ccgctcagcg 1681 tgctttggag gcagcagact tggttgtgga cacaatgatg ttattgcaca gtccagaaca 1741 ggagcagatc ttgaaaacag gcactcgcat cttgttggcc gttgaaccac ctgaggtgtt 1801 ggcacgcatg ttaccaaccg aagatgacaa gcgtcgtgta ttggctgccg aaaccttgtt 1861 gaaacaagcc cgcagtttac atgtgcgttc taaagccggc agtgattttc atgcaccatt 1921 gggtcagtat ccagccgtaa ccgaatatgg ttatgctgac gagcctggcc gttgggatca 1981 ttggccatct ggctttttat ttacttggcc aaacgaggac tcagcagaag gtaccttagt 2041 gttggatgtt ggcgatatta tcttgccatt caaaaactat tgccgcgaac gcatcacttt 2101 ggaaatcgaa aaaggcttca tcaccggtat tcacggtggc tttgaagcag agtacttacg 2161 cgactacatg aagtacttca acgatcctga ggtttatggc attagtcaca tcggttgggg 2221 tttacaacca cgtgcacaat ggactgccat gggcttgcat gaccgcaatg acggtatgtg 2281 tatggatgct cgtgcctttt acggcaattt tttattctct accggtccaa acacagaagt 2341 aggcggtaag cgcaagacac catgccactt ggacatccca ttgcgtaact gcgacattta 2401 tttggacgat aaagccgtag tattggctgg tgacgtggtg gcaccagaag aaagtcgcgc 2461 ccgctaataa tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 2521 actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta ttt //