##gff-version 3
##sequence-region BBa_K1813032 1 5255
BBa_K1813032	.	engineered_region	3569	5255	.	+	0	ID=BBa_K1813013;Name=BBa_K1813013
BBa_K1813032	.	terminator	5161	5255	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813013
BBa_K1813032	.	terminator	5161	5255	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813032	.	terminator	5210	5255	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813032	.	stem_loop	5222	5244	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813032	.	engineered_region	5210	5255	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813032	.	terminator	5161	5201	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813032	.	engineered_region	5161	5201	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813032	.	stop_codon	5194	5194	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813032	.	stem_loop	5168	5187	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813032	.	polyA_site	5188	5201	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813032	.	promoter	3569	3629	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813013
BBa_K1813032	.	promoter	3597	3601	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813032	.	operator	3604	3629	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813032	.	promoter	3569	3608	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813032	.	promoter	3575	3580	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
BBa_K1813032	.	CDS	3656	5152	.	+	0	ID=BBa_K1813005;Name=BBa_K1813005;Parent=BBa_K1813013
BBa_K1813032	.	ribosome_entry_site	3638	3649	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813013
BBa_K1813032	.	conserved_region	3642	3645	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1813032	.	engineered_region	1	3560	.	+	0	ID=BBa_K1813028;Name=BBa_K1813028
BBa_K1813032	.	promoter	1	1319	.	+	0	ID=BBa_K1813019;Name=BBa_K1813019;Parent=BBa_K1813028
BBa_K1813032	.	engineered_region	1328	3560	.	+	0	ID=BBa_K1813018;Name=BBa_K1813018;Parent=BBa_K1813028
BBa_K1813032	.	terminator	3466	3560	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813018
BBa_K1813032	.	terminator	3466	3560	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813032	.	terminator	3515	3560	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813032	.	stem_loop	3527	3549	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813032	.	engineered_region	3515	3560	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813032	.	terminator	3466	3506	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813032	.	engineered_region	3466	3506	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813032	.	stop_codon	3499	3499	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813032	.	stem_loop	3473	3492	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813032	.	polyA_site	3493	3506	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813032	.	promoter	1328	1388	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813018
BBa_K1813032	.	promoter	1356	1360	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813032	.	operator	1363	1388	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813032	.	promoter	1328	1367	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813032	.	promoter	1334	1339	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
BBa_K1813032	.	ribosome_entry_site	1397	1408	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813018
BBa_K1813032	.	conserved_region	1401	1404	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1813032	.	CDS	1415	3457	.	+	0	ID=BBa_K1813010;Name=BBa_K1813010;Parent=BBa_K1813018
>BBa_K1813032
aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccga
aggtgagccagtgtgactctagtagagatctacactagcactatcagagttattaagctactaaagcgtagttttcgtc
gtttgcagcctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag
gcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcct
ggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacgg
cgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcg
gtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagca
tttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagt
gagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctgg
tgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtct
ggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcgg
atagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgt
tctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgt
gcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaat
gtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgg
gaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccatctagtatt
tctcctctttctctagtaggcgctatcatgccataccgcgaaaggttttgcaccatactagaggagctgttgacaatta
atcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatggacgc
tcagaccgaaaccgaagtgccagcaggctctgtgagtgatgaacctttcttaggtcctttagacatcgttttattggta
tctttattggccggtacagcatggtatttgttgaaaggcaagaaaaaagaaagtcaggcatcacagtttaaatcatata
gtattcaaccaacaaccgtgaatacaatgaccatggtggaaaacagtttcatcaagaaattacagagttctggtcgtcg
cttagttgttttttatggttcacagaccggtacagcagaagaatttgccggtcgcttagctaaagaaggcatccgttat
cagatgaaaggtatggtagcagatccagaagaatgtaacatggaagagttgttaatgttaaaagacatcgacaagtcat
tggctgtgttttgcttagcaacctatggtgaaggtgatccaaccgataactgtatggaattttatgattggatccagaa
caatgatttagatatgactggcttgaattacgctgtgtttggtttgggtaataaaacctacgagcattataataaagtt
ggcatttacgttgataaacgcttggaagaattgggtgcaaaccgtgtttttgaattgggcttgggcgatgacgatgcca
acattgaagattatttcatcacctggaaagaaaagttttggccaacagtttgcgattatttcggtattgaaagtacagg
cgaagacgttttgatgcgtcagtatcgcttattggagcaaccagatgtgtcagccgatcgcatttacaccggcgaggtg
gcacgcttacacagtttgcaaacccaacgcccaccttttgacgccaaaaaccctttcttggctccaatcaaagttaatc
gtgagttgcataaagcaggtggccgtagttgcatgcatgtggaatttgatatcgaaggtagtaaaatgcgttatgaggc
tggtgaccatttagccatgtatcctgttaacgatcgcgatttggttgagcgtttaggtcgtttatgtaacgctgaattg
gacaccgttttctctttaatcaacacagacacagattcttcaaaaaaacatccattcccttgcccaaccacttaccgta
ctgccttaacccactacttagaaatcacagccttgccacgtacccacattttgaaagaattagctgaatattgcggtga
ggaaaaagataaagaatttttgcgttttatctcttctaccgcaccagatggcaaggcaaagtatcaggagtgggttcag
gatagttgtcgcaacatcgtacacgtgttggaagacattccttcttgccaccctcctattgaccacgtgtgtgagttat
taccacgtttacaacctcgctaccactctatttcttctagttcaaaattacaccctaccacagttcatgtgaccgcagt
attagtaaaatatgaaaccaaaaccggtcgcttaaataaaggtgtagctaccactttcttggccgagaaacatccaaat
gatggcgaaccagccccacgtgttccaatttttatccgcaaatctcagtttcgtttgcctccaaaaccagaaaccccag
tgattatggtgggcccaggtaccggtttggctcctttccgtggttttattcaggagcgcgaccattgcaaacaggaggg
caaagaaattggtcagaccactttatattttggctgtcgcaaacgtagtgaagactatatctatgaagacgaattggag
gactacagtaaacgcggcatcatcaatttacgtgtggccttttcacgcgatcaagagaaaaaagtgtatgtgactcact
tattagaacaggactctgatttgatttggagtgtgatcggtgaaaataagggtcatttctatatttgcggtgacgccaa
aaacatggctactgatgtgcgcaacatcttgttgaaagtaattcgtagtaaaggcggcttatcagagaccgaagctcaa
caatacatcaagaagatggaggcacagaagcgttattctgctgatgtatggtcataataatactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttat
tattttactagaggagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttacta
gagaaagaggagaaatactagatgggcttagaagcattagttccattagcagtgatcgtggcaatttttttattgttgg
tggatttaatgcatcgccgtcagcgctgggcagcccgttatccacctggccctttgccattgcctggtttaggcaactt
attacatgtggactttcaaaacactccttactgttttgatcaattacgtcgtcgctttggtgacgtattctcattacaa
ttggcatggaccccagttgttgtgttgaatggcttggcagctgtgcgcgaggctttagttacccacggcgaggataccg
ccgaccgtcctccagttcctatcactcagatcttgggctttggccctcgtagtcaaggcgttttcttagcacgctacgg
cccagcttggcgtgaacagcgccgctttagtgtgtctaccttacgtaatttgggtttgggtaaaaaatcattggagcag
tgggttaccgaggaagccgcttgcttgtgcgccgcctttgccaaccactcaggtcgtcctttccgccctaatggtttgt
tggataaagctgtgtcaaacgtgatcgcatcattgacatgcggtcgccgttttgagtacgatgatcctcgctttttacg
tttgttggatttagcacaggaaggtttaaaggaagaatctggttttttgcgtgaggtgttaaacgccgtgcctgtgttg
ttacacattcctgcattagcaggtaaagtattacgcttccaaaaggctttcttgacacaattagatgaattattgactg
aacaccgcatgacctgggacccagcacagccaccacgcgatttaaccgaagcttttttggccgaaatggaaaaagctaa
aggcaacccagaatcatctttcaatgacgaaaacttacgtattgtagttgcagatttattttctgccggcatggtaacc
acttcaaccactttggcctggggcttgttattgatgatcttgcaccctgacgtgcaacgtcgtgttcaacaggagatcg
atgacgttatcggtcaggttcgtcgtccagagatgggtgatcaggcacatatgccttatacaactgctgtgatccatga
agtacagcgttttggcgatattgtgcctttaggcatgacccatatgaccagtcgcgatattgaggtgcaaggtttccgc
attccaaagggcaccacattgattaccaacttatcatctgttttgaaagatgaagccgtgtgggaaaaaccattccgtt
tccatcctgagcattttttggatgcccaaggtcatttcgttaaaccagaagcatttttacctttttcagcaggtcgtcg
tgcatgcttaggtgagcctttggcacgtatggagttattcttattctttaccagtttgttacagcattttagttttagt
gtgcctaccggccagccacgtcctagtcaccatggtgtattcgcctttttggtttcaccaagtccatatgaattgtgcg
cagttccacgctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagaga
atataaaaagccagattattaatccggcttttttattattt