##gff-version 3 ##sequence-region BBa_K1813036 1 6728 BBa_K1813036 . engineered_region 4940 6728 . + 0 ID=BBa_K1813044;Name=BBa_K1813044 BBa_K1813036 . engineered_region 4940 5774 . + 0 ID=BBa_K1813017;Name=BBa_K1813017;Parent=BBa_K1813044 BBa_K1813036 . promoter 4940 5000 . + 0 ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813017 BBa_K1813036 . promoter 4968 4972 . + 0 ID=annotation2197238;Name=-10;Parent=BBa_K864400 BBa_K1813036 . promoter 4946 4951 . + 0 ID=annotation2197237;Name=-35;Parent=BBa_K864400 BBa_K1813036 . promoter 4940 4979 . + 0 ID=annotation2197236;Name=Ptac;Parent=BBa_K864400 BBa_K1813036 . operator 4975 5000 . + 0 ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400 BBa_K1813036 . ribosome_entry_site 5009 5020 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813017 BBa_K1813036 . conserved_region 5013 5016 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1813036 . CDS 5027 5671 . + 0 ID=BBa_K1813009;Name=BBa_K1813009;Parent=BBa_K1813017 BBa_K1813036 . terminator 5680 5774 . + 0 ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813017 BBa_K1813036 . terminator 5680 5774 . + 0 ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017 BBa_K1813036 . terminator 5729 5774 . + 0 ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014 BBa_K1813036 . engineered_region 5729 5774 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_K1813036 . stem_loop 5741 5763 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_K1813036 . terminator 5680 5720 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014 BBa_K1813036 . polyA_site 5707 5720 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1813036 . stem_loop 5687 5706 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1813036 . engineered_region 5680 5720 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1813036 . stop_codon 5713 5713 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1813036 . engineered_region 5783 6728 . + 0 ID=BBa_K1813016;Name=BBa_K1813016;Parent=BBa_K1813044 BBa_K1813036 . ribosome_entry_site 5852 5863 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813016 BBa_K1813036 . conserved_region 5856 5859 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1813036 . terminator 6634 6728 . + 0 ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813016 BBa_K1813036 . terminator 6634 6728 . + 0 ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017 BBa_K1813036 . terminator 6683 6728 . + 0 ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014 BBa_K1813036 . engineered_region 6683 6728 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_K1813036 . stem_loop 6695 6717 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_K1813036 . terminator 6634 6674 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014 BBa_K1813036 . polyA_site 6661 6674 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1813036 . stem_loop 6641 6660 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1813036 . engineered_region 6634 6674 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1813036 . stop_codon 6667 6667 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1813036 . promoter 5783 5843 . + 0 ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813016 BBa_K1813036 . promoter 5811 5815 . + 0 ID=annotation2197238;Name=-10;Parent=BBa_K864400 BBa_K1813036 . promoter 5789 5794 . + 0 ID=annotation2197237;Name=-35;Parent=BBa_K864400 BBa_K1813036 . promoter 5783 5822 . + 0 ID=annotation2197236;Name=Ptac;Parent=BBa_K864400 BBa_K1813036 . operator 5818 5843 . + 0 ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400 BBa_K1813036 . CDS 5870 6625 . + 0 ID=BBa_K1813008;Name=BBa_K1813008;Parent=BBa_K1813016 BBa_K1813036 . engineered_region 1 4931 . + 0 ID=BBa_K1813049;Name=BBa_K1813049 BBa_K1813036 . engineered_region 1 3923 . + 0 ID=BBa_K1813042;Name=BBa_K1813042;Parent=BBa_K1813049 BBa_K1813036 . engineered_region 2678 3923 . + 0 ID=BBa_K1813030;Name=BBa_K1813030;Parent=BBa_K1813042 BBa_K1813036 . ribosome_entry_site 2747 2758 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813030 BBa_K1813036 . conserved_region 2751 2754 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1813036 . promoter 2678 2738 . + 0 ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813030 BBa_K1813036 . promoter 2706 2710 . + 0 ID=annotation2197238;Name=-10;Parent=BBa_K864400 BBa_K1813036 . promoter 2684 2689 . + 0 ID=annotation2197237;Name=-35;Parent=BBa_K864400 BBa_K1813036 . promoter 2678 2717 . + 0 ID=annotation2197236;Name=Ptac;Parent=BBa_K864400 BBa_K1813036 . operator 2713 2738 . + 0 ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400 BBa_K1813036 . terminator 3829 3923 . + 0 ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813030 BBa_K1813036 . terminator 3829 3923 . + 0 ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017 BBa_K1813036 . terminator 3878 3923 . + 0 ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014 BBa_K1813036 . engineered_region 3878 3923 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_K1813036 . stem_loop 3890 3912 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_K1813036 . terminator 3829 3869 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014 BBa_K1813036 . polyA_site 3856 3869 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1813036 . stem_loop 3836 3855 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1813036 . engineered_region 3829 3869 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1813036 . stop_codon 3862 3862 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1813036 . CDS 2765 3820 . + 0 ID=BBa_K1813029;Name=BBa_K1813029;Parent=BBa_K1813030 BBa_K1813036 . promoter 1 1319 . + 0 ID=BBa_K1813019;Name=BBa_K1813019;Parent=BBa_K1813042 BBa_K1813036 . engineered_region 1328 2669 . + 0 ID=BBa_K1813014;Name=BBa_K1813014;Parent=BBa_K1813042 BBa_K1813036 . terminator 2575 2669 . + 0 ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813014 BBa_K1813036 . terminator 2575 2669 . + 0 ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017 BBa_K1813036 . terminator 2624 2669 . + 0 ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014 BBa_K1813036 . engineered_region 2624 2669 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_K1813036 . stem_loop 2636 2658 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_K1813036 . terminator 2575 2615 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014 BBa_K1813036 . polyA_site 2602 2615 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1813036 . stem_loop 2582 2601 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1813036 . engineered_region 2575 2615 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1813036 . stop_codon 2608 2608 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1813036 . CDS 1415 2566 . + 0 ID=BBa_K1813006;Name=BBa_K1813006;Parent=BBa_K1813014 BBa_K1813036 . promoter 1328 1388 . + 0 ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813014 BBa_K1813036 . promoter 1356 1360 . + 0 ID=annotation2197238;Name=-10;Parent=BBa_K864400 BBa_K1813036 . promoter 1334 1339 . + 0 ID=annotation2197237;Name=-35;Parent=BBa_K864400 BBa_K1813036 . promoter 1328 1367 . + 0 ID=annotation2197236;Name=Ptac;Parent=BBa_K864400 BBa_K1813036 . operator 1363 1388 . + 0 ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400 BBa_K1813036 . ribosome_entry_site 1397 1408 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813014 BBa_K1813036 . conserved_region 1401 1404 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1813036 . engineered_region 3932 4931 . + 0 ID=BBa_K1813015;Name=BBa_K1813015;Parent=BBa_K1813049 BBa_K1813036 . terminator 4837 4931 . + 0 ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813015 BBa_K1813036 . terminator 4837 4931 . + 0 ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017 BBa_K1813036 . terminator 4886 4931 . + 0 ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014 BBa_K1813036 . engineered_region 4886 4931 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_K1813036 . stem_loop 4898 4920 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_K1813036 . terminator 4837 4877 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014 BBa_K1813036 . polyA_site 4864 4877 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1813036 . stem_loop 4844 4863 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1813036 . engineered_region 4837 4877 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1813036 . stop_codon 4870 4870 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1813036 . CDS 4019 4828 . + 0 ID=BBa_K1813007;Name=BBa_K1813007;Parent=BBa_K1813015 BBa_K1813036 . promoter 3932 3992 . + 0 ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813015 BBa_K1813036 . promoter 3960 3964 . + 0 ID=annotation2197238;Name=-10;Parent=BBa_K864400 BBa_K1813036 . promoter 3938 3943 . + 0 ID=annotation2197237;Name=-35;Parent=BBa_K864400 BBa_K1813036 . promoter 3932 3971 . + 0 ID=annotation2197236;Name=Ptac;Parent=BBa_K864400 BBa_K1813036 . operator 3967 3992 . + 0 ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400 BBa_K1813036 . ribosome_entry_site 4001 4012 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813015 BBa_K1813036 . conserved_region 4005 4008 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 >BBa_K1813036 aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccga aggtgagccagtgtgactctagtagagatctacactagcactatcagagttattaagctactaaagcgtagttttcgtc gtttgcagcctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag gcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcct ggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacgg cgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcg gtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagca tttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagt gagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctgg tgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtct ggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcgg atagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgt tctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgt gcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaat gtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgg gaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccatctagtatt tctcctctttctctagtaggcgctatcatgccataccgcgaaaggttttgcaccatactagaggagctgttgacaatta atcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgcgcgg tcgccagaagatcgcaattgttggtgctggcttaggcggcgcagccgctgctactttattgcagcaagctggttttgat gttgaagtgtttgagcaggccccagcattcacccgcttaggcgctggcatccacatcggccctaacgttatgaagattt ttcgccgtatgggtttagagcagaaattagagttaatgggctctcacccagacttctggttttcacgtgacggtaacac cggtgactacttgtcacgcattccattaggcgagttcgcccgccgcgagtatggcgctgcctacattaccatccaccgc ggcgatttacacgcattgcagattgaagcaattcagccaggtaccgttcactttggcaaacgtttagaaaaaattgttg atgaaggtgaccaggttcgtttagacttcgctgacggtacccacaccgtggcagacattgtgatcggcgcagacggcat tcattcaaagattcgcgaagagttattgggtgccgaagccccaatctattctggttgggtggctcatcgtgcattgatt cgtggtgtgaatttagcccaacacgccgatgttttcgagccatgcgttaagtggtggagtgaagaccgtcatatgatgg tgtattatactacaggcaagcgtgatgaatattatttcgttaccggcgtgcctcacgaagcctgggattttcaaggcgc attcgtggacagttctcaggaagagatgcgcgcagccttcgaaggttaccacccaacagtgcaaaaattgatcgacgct accgaatcaatcacaaaatggcctttacgcaaccgcaatcctttacctttgtggtcacgtggccgcttagttttattgg gcgacgcttgtcatcctatgaaacctcatatggcacagggcgcttgcatggctattgaggatgcagcaatgttaacccg ttgtttacaggaaactggcttgagtgatcaccgcacagctttcgcattatacgaagcaaaccgcaaggaacgcgcctca caggttcagagtgtttctaatgccaacacttggttatattctcaggaagatcctgcctgggtatatggttatgacttgt atggtcagcagttagaaagtggcgaagctgcttaataatactagagtcacactggctcaccttcgggtgggcctttctg cgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggagctgttg acaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactaga tgccagtaagtaatgcacagttaacccagatgttcgaacacgtgttaaaattaagtcgcgtggatgaaactcagtctgt tgcagtgttaaaaagtcattatagtgaccctcgcactgtgaacgcagctatggaggcagcacaacgtttgaaagctaag gtttatgccgttgaattacctgcatttaaccatccaaccgctatgggtaatgatatgaccgcctattgcggcgataccg cattaactggtaacttagccgctcagcgtgctttggaggcagcagacttggttgtggacacaatgatgttattgcacag tccagaacaggagcagatcttgaaaacaggcactcgcatcttgttggccgttgaaccacctgaggtgttggcacgcatg ttaccaaccgaagatgacaagcgtcgtgtattggctgccgaaaccttgttgaaacaagcccgcagtttacatgtgcgtt ctaaagccggcagtgattttcatgcaccattgggtcagtatccagccgtaaccgaatatggttatgctgacgagcctgg ccgttgggatcattggccatctggctttttatttacttggccaaacgaggactcagcagaaggtaccttagtgttggat gttggcgatattatcttgccattcaaaaactattgccgcgaacgcatcactttggaaatcgaaaaaggcttcatcaccg gtattcacggtggctttgaagcagagtacttacgcgactacatgaagtacttcaacgatcctgaggtttatggcattag tcacatcggttggggtttacaaccacgtgcacaatggactgccatgggcttgcatgaccgcaatgacggtatgtgtatg gatgctcgtgccttttacggcaattttttattctctaccggtccaaacacagaagtaggcggtaagcgcaagacaccat gccacttggacatcccattgcgtaactgcgacatttatttggacgataaagccgtagtattggctggtgacgtggtggc accagaagaaagtcgcgcccgctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata ctagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggagctgttgacaattaatc atcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtacctt tgtggcaggcggcaacgtatctgccaacggcattcgtcaacactatttgcgttatggcggcaaaggtcacgctttaatc ttagttccaggcattacatctcctgccatcacatggggcttcgtggctgaacgcttaggtcattactttgacacctatg tattggacgttcgtggtcgtggcttatctagttcaggccctgacttggattacggcactgacgcatgcgccgccgacat cccagcttttgccgcagccttgggtttggatagttaccacttattaggtcattctatgggcgcccgttttgcaatccgc gccgccgcacaaggcgccccaggcttgcagcgcttagtgttagtagaccctccagtaagtggtccaggccgtcgtgcat acccatcaaaattaccttggtacgtggattctattcgccaagctaccgtaggcatgagtggtgacgatatgcgcgcctt ctgtgctacttggtcagatgaacagttagctttacgcgcagagtggttacacacatgttatgaaccagccatcgtgcgt gcatttgacgattttcacgaagttgatattcaccagtatttacctgctgtgcgccagcctgccttattaatggttgccg gtcgtggcggcgttattgaaccacgtgatattgccgaaatgcgtgagttgaaaccagacatccaagtggcctacgttga taatgccggccacatgatcccatgggatgatttagacggtttcttcgccgcattcggcgatttcttagatcacccattg gtttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaa agccagattattaatccggcttttttattattttactagaggagctgttgacaattaatcatcggctcgtataatgtgt ggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtgatgctcaatcagcacgtgataat tatcagggcgtgtggggccagcgtatcggttttggccgtaaaccagcattattaatgatcgacttcatgcagggctaca caacccctggcgcacctttatacgctcctggtgtagttgcagcagtggagcaagcagcaggtttgttggctttggcccg cgactgtggcacattagtggtgcatacaaacattcgctatcagcctccacatttcgccgatggtggtgtgtgggttcgt aaggcccctgtgatgaaagacatggttgaaggcaaccctttagctgccttctgtgaagccgtagcacctcaggcaggcg aagtagtattgtctaaacagtatgcaagtgccttttttgccacttcattggccccattattacatgcccagggtgttga tactgtggtattggccggctgtagtacctctggttgcattcgtgcaagtgctgtggatgctatgcagcacggcttccgc acaatcgtagtgcgcgaatgcgtaggtgaccgccatagtgacccacatgaagcaaacttattcgatattgacagtaaat atggcgatgtggttacccgtcaagatgctatgcagcagttacgccacttagcaggctaataatactagagtcacactgg ctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggctttttt attattttactagaggagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttac tagagaaagaggagaaatactagatgacccagttatatcgtatcggtcagattgtacctagttctaataccacaatgga gaccgaaattcctgctatgttgaatgcccgtcaggctatccgccctgaacgttttacttttcactcatctcgcatgcgc atgaaacaagtaaaaaaagaagaattagctgctatggatgcagagagtgaccgctgcgcagttgaattgtctgatgcaa aagtggatgtattaggttatgcatgcttagtagcaattatggcaatgggcttaggctaccatcgccagtcagagaaacg cttacagcaagcaaccgccgataacgatgcattggcccctgtgatcacttcagcaggcgccttagtggaagcattacat gtgatgaaggccaaacgcatcgccattgtagcaccatatatgaagccattgaccgaattagtggttaactacatccgtg aagaaggcttcgaggttcaggactggcgtgcattagaaatccctgataatttggccgtagcccgccatgatccagctaa cttgcctggtattgtggcaggtatggatttggaaggtgttgatgtagttgttttatctgcatgtgtgcaaatgcagtca ttacctgccgtggccaaggttgaagcccaaaccggtaaacctgttgtgacagccgccatcgcaaccacctacgccatgt tgaaagccttagatttagagcctattgtacctggcgcaggcgctttgttatctggtgcttactaataatactagagtca cactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggc ttttttattattt