BBa_K1813037
1
BBa_K1813037
ptac and RBS
2015-09-10T11:00:00Z
2015-09-18T07:18:03Z
Registry
ptac RBS
false
false
_2238_
25865
12892
9
false
NA
false
UBC iGEM 2015
component2449457
1
BBa_K864400
component2449459
1
BBa_B0034
annotation2449457
1
BBa_K864400
range2449457
1
1
61
annotation2449459
1
BBa_B0034
range2449459
1
70
81
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K864400
1
BBa_K864400
Ptac, trp & lac regulated promoter
2012-09-24T11:00:00Z
2015-05-08T01:13:37Z
Created from oligos ordered from SigmaAldrich.
Oligo sequences:
Ptac+
AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA
Ptac-
CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
Released HQ 2013
The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]]
[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
false
false
_1124_
0
9827
9
In stock
false
Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.
false
Erik Lundin
annotation2197238
1
-10
range2197238
1
29
33
annotation2197237
1
-35
range2197237
1
7
12
annotation2197236
1
Ptac
range2197236
1
1
40
annotation2197240
1
lacO1 site
range2197240
1
36
61
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1813037_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaa
BBa_K864400_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z