LOCUS       BBa_K1813043            2254 bp    DNA     linear       27-Jun-2024
DEFINITION  <i>nicX and nicD</i> Expression Cassette
ACCESSION   BBa_K1813043
VERSION     BBa_K1813043.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     CDS             88..1143
                     /label=BBa_K1813029
     stem_loop       1159..1178
                     /label=T7 TE
     misc_feature    1185..1185
                     /label=stop
     polyA_site      1179..1192
                     /label=polya
     misc_feature    1152..1192
                     /label=BBa_B0012
     terminator      1152..1192
                     /label=BBa_B0012
     stem_loop       1213..1235
                     /label=stem_loop
     misc_feature    1201..1246
                     /label=BBa_B0011
     terminator      1201..1246
                     /label=BBa_B0011
     terminator      1152..1246
                     /label=BBa_B0014
     terminator      1152..1246
                     /label=BBa_K823017
     misc_feature    1..1246
                     /label=BBa_K1813030
     promoter        1255..1294
                     /label=Ptac
     promoter        1261..1266
                     /label=-35
     promoter        1283..1287
                     /label=-10
     misc_feature    1290..1315
                     /label=lacO1 site
     promoter        1255..1315
                     /label=BBa_K864400
     misc_feature    1328..1331
                     /label=conserved
     RBS             1324..1335
                     /label=BBa_B0034
     CDS             1342..2151
                     /label=BBa_K1813007
     stem_loop       2167..2186
                     /label=T7 TE
     misc_feature    2193..2193
                     /label=stop
     polyA_site      2187..2200
                     /label=polya
     misc_feature    2160..2200
                     /label=BBa_B0012
     terminator      2160..2200
                     /label=BBa_B0012
     stem_loop       2221..2243
                     /label=stem_loop
     misc_feature    2209..2254
                     /label=BBa_B0011
     terminator      2209..2254
                     /label=BBa_B0011
     terminator      2160..2254
                     /label=BBa_B0014
     terminator      2160..2254
                     /label=BBa_K823017
     misc_feature    1255..2254
                     /label=BBa_K1813015
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg ccagtaagta atgcacagtt aacccagatg
      121 ttcgaacacg tgttaaaatt aagtcgcgtg gatgaaactc agtctgttgc agtgttaaaa
      181 agtcattata gtgaccctcg cactgtgaac gcagctatgg aggcagcaca acgtttgaaa
      241 gctaaggttt atgccgttga attacctgca tttaaccatc caaccgctat gggtaatgat
      301 atgaccgcct attgcggcga taccgcatta actggtaact tagccgctca gcgtgctttg
      361 gaggcagcag acttggttgt ggacacaatg atgttattgc acagtccaga acaggagcag
      421 atcttgaaaa caggcactcg catcttgttg gccgttgaac cacctgaggt gttggcacgc
      481 atgttaccaa ccgaagatga caagcgtcgt gtattggctg ccgaaacctt gttgaaacaa
      541 gcccgcagtt tacatgtgcg ttctaaagcc ggcagtgatt ttcatgcacc attgggtcag
      601 tatccagccg taaccgaata tggttatgct gacgagcctg gccgttggga tcattggcca
      661 tctggctttt tatttacttg gccaaacgag gactcagcag aaggtacctt agtgttggat
      721 gttggcgata ttatcttgcc attcaaaaac tattgccgcg aacgcatcac tttggaaatc
      781 gaaaaaggct tcatcaccgg tattcacggt ggctttgaag cagagtactt acgcgactac
      841 atgaagtact tcaacgatcc tgaggtttat ggcattagtc acatcggttg gggtttacaa
      901 ccacgtgcac aatggactgc catgggcttg catgaccgca atgacggtat gtgtatggat
      961 gctcgtgcct tttacggcaa ttttttattc tctaccggtc caaacacaga agtaggcggt
     1021 aagcgcaaga caccatgcca cttggacatc ccattgcgta actgcgacat ttatttggac
     1081 gataaagccg tagtattggc tggtgacgtg gtggcaccag aagaaagtcg cgcccgctaa
     1141 taatactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1201 agagaatata aaaagccaga ttattaatcc ggctttttta ttattttact agaggagctg
     1261 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttacta
     1321 gagaaagagg agaaatacta gatgagtacc tttgtggcag gcggcaacgt atctgccaac
     1381 ggcattcgtc aacactattt gcgttatggc ggcaaaggtc acgctttaat cttagttcca
     1441 ggcattacat ctcctgccat cacatggggc ttcgtggctg aacgcttagg tcattacttt
     1501 gacacctatg tattggacgt tcgtggtcgt ggcttatcta gttcaggccc tgacttggat
     1561 tacggcactg acgcatgcgc cgccgacatc ccagcttttg ccgcagcctt gggtttggat
     1621 agttaccact tattaggtca ttctatgggc gcccgttttg caatccgcgc cgccgcacaa
     1681 ggcgccccag gcttgcagcg cttagtgtta gtagaccctc cagtaagtgg tccaggccgt
     1741 cgtgcatacc catcaaaatt accttggtac gtggattcta ttcgccaagc taccgtaggc
     1801 atgagtggtg acgatatgcg cgccttctgt gctacttggt cagatgaaca gttagcttta
     1861 cgcgcagagt ggttacacac atgttatgaa ccagccatcg tgcgtgcatt tgacgatttt
     1921 cacgaagttg atattcacca gtatttacct gctgtgcgcc agcctgcctt attaatggtt
     1981 gccggtcgtg gcggcgttat tgaaccacgt gatattgccg aaatgcgtga gttgaaacca
     2041 gacatccaag tggcctacgt tgataatgcc ggccacatga tcccatggga tgatttagac
     2101 ggtttcttcg ccgcattcgg cgatttctta gatcacccat tggtttaata atactagagt
     2161 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa
     2221 aagccagatt attaatccgg cttttttatt attt
//