BBa_K864400 1 BBa_K864400 Ptac, trp & lac regulated promoter 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG Released HQ 2013 The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]] [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25 false false _1124_ 0 9827 9 In stock false Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone. false Erik Lundin annotation2197240 1 lacO1 site range2197240 1 36 61 annotation2197238 1 -10 range2197238 1 29 33 annotation2197237 1 -35 range2197237 1 7 12 annotation2197236 1 Ptac range2197236 1 1 40 BBa_K1813029 1 BBa_K1813029 <i>nicX</i> 2015-08-08T11:00:00Z 2015-09-18T06:54:12Z Synthesized nicX false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 BBa_K823017 1 BBa_K823017 double terminator (B0012-B0011) 2012-09-10T11:00:00Z 2015-05-08T01:13:30Z Part:BBa_B0014 Released HQ 2013 This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3. false false _1081_ 0 11555 9 In stock false This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3. false Jara Radeck component2182657 1 BBa_B0014 annotation2182657 1 BBa_B0014 range2182657 1 1 95 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_K1813007 1 BBa_K1813007 <i>nicD</i> 2015-07-26T11:00:00Z 2015-09-18T11:13:27Z Synthesized nicD false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K1813015 1 BBa_K1813015 <i>nicD</i> Expression Casette 2015-07-26T11:00:00Z 2015-09-18T04:09:51Z Synthesized and registry ptac nicD term false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 component2433630 1 BBa_K864400 component2433633 1 BBa_K1813007 component2433632 1 BBa_B0034 component2433641 1 BBa_K823017 annotation2433641 1 BBa_K823017 range2433641 1 906 1000 annotation2433632 1 BBa_B0034 range2433632 1 70 81 annotation2433630 1 BBa_K864400 range2433630 1 1 61 annotation2433633 1 BBa_K1813007 range2433633 1 88 897 BBa_K1813043 1 BBa_K1813043 <i>nicX and nicD</i> Expression Cassette 2015-09-10T11:00:00Z 2015-09-18T09:52:06Z Registry and synthesized ptac nicX nicD false false _2238_ 12892 12892 9 false NA false UBC iGEM 2015 component2449587 1 BBa_K1813030 component2449604 1 BBa_K1813015 annotation2449604 1 BBa_K1813015 range2449604 1 1255 2254 annotation2449587 1 BBa_K1813030 range2449587 1 1 1246 BBa_K1813030 1 BBa_K1813030 <i>nicX<i> Expression Cassette 2015-08-08T11:00:00Z 2015-09-18T04:36:48Z Synthesized and Registry ptac nicX false false _2238_ 12892 12892 9 false NA false UBC iGEM 2015 component2434565 1 BBa_K1813029 component2434562 1 BBa_K864400 component2434564 1 BBa_B0034 component2434573 1 BBa_K823017 annotation2434562 1 BBa_K864400 range2434562 1 1 61 annotation2434564 1 BBa_B0034 range2434564 1 70 81 annotation2434573 1 BBa_K823017 range2434573 1 1152 1246 annotation2434565 1 BBa_K1813029 range2434565 1 88 1143 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939303 1 BBa_B0012 range939303 1 1 41 annotation939311 1 BBa_B0011 range939311 1 50 95 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813007_sequence 1 atgagtacctttgtggcaggcggcaacgtatctgccaacggcattcgtcaacactatttgcgttatggcggcaaaggtcacgctttaatcttagttccaggcattacatctcctgccatcacatggggcttcgtggctgaacgcttaggtcattactttgacacctatgtattggacgttcgtggtcgtggcttatctagttcaggccctgacttggattacggcactgacgcatgcgccgccgacatcccagcttttgccgcagccttgggtttggatagttaccacttattaggtcattctatgggcgcccgttttgcaatccgcgccgccgcacaaggcgccccaggcttgcagcgcttagtgttagtagaccctccagtaagtggtccaggccgtcgtgcatacccatcaaaattaccttggtacgtggattctattcgccaagctaccgtaggcatgagtggtgacgatatgcgcgccttctgtgctacttggtcagatgaacagttagctttacgcgcagagtggttacacacatgttatgaaccagccatcgtgcgtgcatttgacgattttcacgaagttgatattcaccagtatttacctgctgtgcgccagcctgccttattaatggttgccggtcgtggcggcgttattgaaccacgtgatattgccgaaatgcgtgagttgaaaccagacatccaagtggcctacgttgataatgccggccacatgatcccatgggatgatttagacggtttcttcgccgcattcggcgatttcttagatcacccattggtttaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1813029_sequence 1 atgccagtaagtaatgcacagttaacccagatgttcgaacacgtgttaaaattaagtcgcgtggatgaaactcagtctgttgcagtgttaaaaagtcattatagtgaccctcgcactgtgaacgcagctatggaggcagcacaacgtttgaaagctaaggtttatgccgttgaattacctgcatttaaccatccaaccgctatgggtaatgatatgaccgcctattgcggcgataccgcattaactggtaacttagccgctcagcgtgctttggaggcagcagacttggttgtggacacaatgatgttattgcacagtccagaacaggagcagatcttgaaaacaggcactcgcatcttgttggccgttgaaccacctgaggtgttggcacgcatgttaccaaccgaagatgacaagcgtcgtgtattggctgccgaaaccttgttgaaacaagcccgcagtttacatgtgcgttctaaagccggcagtgattttcatgcaccattgggtcagtatccagccgtaaccgaatatggttatgctgacgagcctggccgttgggatcattggccatctggctttttatttacttggccaaacgaggactcagcagaaggtaccttagtgttggatgttggcgatattatcttgccattcaaaaactattgccgcgaacgcatcactttggaaatcgaaaaaggcttcatcaccggtattcacggtggctttgaagcagagtacttacgcgactacatgaagtacttcaacgatcctgaggtttatggcattagtcacatcggttggggtttacaaccacgtgcacaatggactgccatgggcttgcatgaccgcaatgacggtatgtgtatggatgctcgtgccttttacggcaattttttattctctaccggtccaaacacagaagtaggcggtaagcgcaagacaccatgccacttggacatcccattgcgtaactgcgacatttatttggacgataaagccgtagtattggctggtgacgtggtggcaccagaagaaagtcgcgcccgctaataa BBa_K823017_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813030_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgccagtaagtaatgcacagttaacccagatgttcgaacacgtgttaaaattaagtcgcgtggatgaaactcagtctgttgcagtgttaaaaagtcattatagtgaccctcgcactgtgaacgcagctatggaggcagcacaacgtttgaaagctaaggtttatgccgttgaattacctgcatttaaccatccaaccgctatgggtaatgatatgaccgcctattgcggcgataccgcattaactggtaacttagccgctcagcgtgctttggaggcagcagacttggttgtggacacaatgatgttattgcacagtccagaacaggagcagatcttgaaaacaggcactcgcatcttgttggccgttgaaccacctgaggtgttggcacgcatgttaccaaccgaagatgacaagcgtcgtgtattggctgccgaaaccttgttgaaacaagcccgcagtttacatgtgcgttctaaagccggcagtgattttcatgcaccattgggtcagtatccagccgtaaccgaatatggttatgctgacgagcctggccgttgggatcattggccatctggctttttatttacttggccaaacgaggactcagcagaaggtaccttagtgttggatgttggcgatattatcttgccattcaaaaactattgccgcgaacgcatcactttggaaatcgaaaaaggcttcatcaccggtattcacggtggctttgaagcagagtacttacgcgactacatgaagtacttcaacgatcctgaggtttatggcattagtcacatcggttggggtttacaaccacgtgcacaatggactgccatgggcttgcatgaccgcaatgacggtatgtgtatggatgctcgtgccttttacggcaattttttattctctaccggtccaaacacagaagtaggcggtaagcgcaagacaccatgccacttggacatcccattgcgtaactgcgacatttatttggacgataaagccgtagtattggctggtgacgtggtggcaccagaagaaagtcgcgcccgctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813015_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtacctttgtggcaggcggcaacgtatctgccaacggcattcgtcaacactatttgcgttatggcggcaaaggtcacgctttaatcttagttccaggcattacatctcctgccatcacatggggcttcgtggctgaacgcttaggtcattactttgacacctatgtattggacgttcgtggtcgtggcttatctagttcaggccctgacttggattacggcactgacgcatgcgccgccgacatcccagcttttgccgcagccttgggtttggatagttaccacttattaggtcattctatgggcgcccgttttgcaatccgcgccgccgcacaaggcgccccaggcttgcagcgcttagtgttagtagaccctccagtaagtggtccaggccgtcgtgcatacccatcaaaattaccttggtacgtggattctattcgccaagctaccgtaggcatgagtggtgacgatatgcgcgccttctgtgctacttggtcagatgaacagttagctttacgcgcagagtggttacacacatgttatgaaccagccatcgtgcgtgcatttgacgattttcacgaagttgatattcaccagtatttacctgctgtgcgccagcctgccttattaatggttgccggtcgtggcggcgttattgaaccacgtgatattgccgaaatgcgtgagttgaaaccagacatccaagtggcctacgttgataatgccggccacatgatcccatgggatgatttagacggtttcttcgccgcattcggcgatttcttagatcacccattggtttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813043_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgccagtaagtaatgcacagttaacccagatgttcgaacacgtgttaaaattaagtcgcgtggatgaaactcagtctgttgcagtgttaaaaagtcattatagtgaccctcgcactgtgaacgcagctatggaggcagcacaacgtttgaaagctaaggtttatgccgttgaattacctgcatttaaccatccaaccgctatgggtaatgatatgaccgcctattgcggcgataccgcattaactggtaacttagccgctcagcgtgctttggaggcagcagacttggttgtggacacaatgatgttattgcacagtccagaacaggagcagatcttgaaaacaggcactcgcatcttgttggccgttgaaccacctgaggtgttggcacgcatgttaccaaccgaagatgacaagcgtcgtgtattggctgccgaaaccttgttgaaacaagcccgcagtttacatgtgcgttctaaagccggcagtgattttcatgcaccattgggtcagtatccagccgtaaccgaatatggttatgctgacgagcctggccgttgggatcattggccatctggctttttatttacttggccaaacgaggactcagcagaaggtaccttagtgttggatgttggcgatattatcttgccattcaaaaactattgccgcgaacgcatcactttggaaatcgaaaaaggcttcatcaccggtattcacggtggctttgaagcagagtacttacgcgactacatgaagtacttcaacgatcctgaggtttatggcattagtcacatcggttggggtttacaaccacgtgcacaatggactgccatgggcttgcatgaccgcaatgacggtatgtgtatggatgctcgtgccttttacggcaattttttattctctaccggtccaaacacagaagtaggcggtaagcgcaagacaccatgccacttggacatcccattgcgtaactgcgacatttatttggacgataaagccgtagtattggctggtgacgtggtggcaccagaagaaagtcgcgcccgctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtacctttgtggcaggcggcaacgtatctgccaacggcattcgtcaacactatttgcgttatggcggcaaaggtcacgctttaatcttagttccaggcattacatctcctgccatcacatggggcttcgtggctgaacgcttaggtcattactttgacacctatgtattggacgttcgtggtcgtggcttatctagttcaggccctgacttggattacggcactgacgcatgcgccgccgacatcccagcttttgccgcagccttgggtttggatagttaccacttattaggtcattctatgggcgcccgttttgcaatccgcgccgccgcacaaggcgccccaggcttgcagcgcttagtgttagtagaccctccagtaagtggtccaggccgtcgtgcatacccatcaaaattaccttggtacgtggattctattcgccaagctaccgtaggcatgagtggtgacgatatgcgcgccttctgtgctacttggtcagatgaacagttagctttacgcgcagagtggttacacacatgttatgaaccagccatcgtgcgtgcatttgacgattttcacgaagttgatattcaccagtatttacctgctgtgcgccagcctgccttattaatggttgccggtcgtggcggcgttattgaaccacgtgatattgccgaaatgcgtgagttgaaaccagacatccaagtggcctacgttgataatgccggccacatgatcccatgggatgatttagacggtttcttcgccgcattcggcgatttcttagatcacccattggtttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K864400_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z