LOCUS       BBa_K1813044            1789 bp    DNA     linear       09-Jan-2025
DEFINITION  <i>nicF and nicE</i> Expression Cassette
ACCESSION   BBa_K1813044
VERSION     BBa_K1813044.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     CDS             88..732
                     /label=BBa_K1813009
     stem_loop       748..767
                     /label=T7 TE
     misc_feature    774..774
                     /label=stop
     polyA_site      768..781
                     /label=polya
     misc_feature    741..781
                     /label=BBa_B0012
     terminator      741..781
                     /label=BBa_B0012
     stem_loop       802..824
                     /label=stem_loop
     misc_feature    790..835
                     /label=BBa_B0011
     terminator      790..835
                     /label=BBa_B0011
     terminator      741..835
                     /label=BBa_B0014
     terminator      741..835
                     /label=BBa_K823017
     misc_feature    1..835
                     /label=BBa_K1813017
     promoter        844..883
                     /label=Ptac
     promoter        850..855
                     /label=-35
     promoter        872..876
                     /label=-10
     misc_feature    879..904
                     /label=lacO1 site
     promoter        844..904
                     /label=BBa_K864400
     misc_feature    917..920
                     /label=conserved
     RBS             913..924
                     /label=BBa_B0034
     CDS             931..1686
                     /label=BBa_K1813008
     stem_loop       1702..1721
                     /label=T7 TE
     misc_feature    1728..1728
                     /label=stop
     polyA_site      1722..1735
                     /label=polya
     misc_feature    1695..1735
                     /label=BBa_B0012
     terminator      1695..1735
                     /label=BBa_B0012
     stem_loop       1756..1778
                     /label=stem_loop
     misc_feature    1744..1789
                     /label=BBa_B0011
     terminator      1744..1789
                     /label=BBa_B0011
     terminator      1695..1789
                     /label=BBa_B0014
     terminator      1695..1789
                     /label=BBa_K823017
     misc_feature    844..1789
                     /label=BBa_K1813016
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg agtgatgctc aatcagcacg tgataattat
      121 cagggcgtgt ggggccagcg tatcggtttt ggccgtaaac cagcattatt aatgatcgac
      181 ttcatgcagg gctacacaac ccctggcgca cctttatacg ctcctggtgt agttgcagca
      241 gtggagcaag cagcaggttt gttggctttg gcccgcgact gtggcacatt agtggtgcat
      301 acaaacattc gctatcagcc tccacatttc gccgatggtg gtgtgtgggt tcgtaaggcc
      361 cctgtgatga aagacatggt tgaaggcaac cctttagctg ccttctgtga agccgtagca
      421 cctcaggcag gcgaagtagt attgtctaaa cagtatgcaa gtgccttttt tgccacttca
      481 ttggccccat tattacatgc ccagggtgtt gatactgtgg tattggccgg ctgtagtacc
      541 tctggttgca ttcgtgcaag tgctgtggat gctatgcagc acggcttccg cacaatcgta
      601 gtgcgcgaat gcgtaggtga ccgccatagt gacccacatg aagcaaactt attcgatatt
      661 gacagtaaat atggcgatgt ggttacccgt caagatgcta tgcagcagtt acgccactta
      721 gcaggctaat aatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      781 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tattttacta
      841 gaggagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac
      901 aatttactag agaaagagga gaaatactag atgacccagt tatatcgtat cggtcagatt
      961 gtacctagtt ctaataccac aatggagacc gaaattcctg ctatgttgaa tgcccgtcag
     1021 gctatccgcc ctgaacgttt tacttttcac tcatctcgca tgcgcatgaa acaagtaaaa
     1081 aaagaagaat tagctgctat ggatgcagag agtgaccgct gcgcagttga attgtctgat
     1141 gcaaaagtgg atgtattagg ttatgcatgc ttagtagcaa ttatggcaat gggcttaggc
     1201 taccatcgcc agtcagagaa acgcttacag caagcaaccg ccgataacga tgcattggcc
     1261 cctgtgatca cttcagcagg cgccttagtg gaagcattac atgtgatgaa ggccaaacgc
     1321 atcgccattg tagcaccata tatgaagcca ttgaccgaat tagtggttaa ctacatccgt
     1381 gaagaaggct tcgaggttca ggactggcgt gcattagaaa tccctgataa tttggccgta
     1441 gcccgccatg atccagctaa cttgcctggt attgtggcag gtatggattt ggaaggtgtt
     1501 gatgtagttg ttttatctgc atgtgtgcaa atgcagtcat tacctgccgt ggccaaggtt
     1561 gaagcccaaa ccggtaaacc tgttgtgaca gccgccatcg caaccaccta cgccatgttg
     1621 aaagccttag atttagagcc tattgtacct ggcgcaggcg ctttgttatc tggtgcttac
     1681 taataatact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     1741 gagagagaat ataaaaagcc agattattaa tccggctttt ttattattt
//