LOCUS BBa_K1813045 5519 bp DNA linear 22-Dec-2024 DEFINITION LacI Reversed, cch2, nicC and nicX Regulon ACCESSION BBa_K1813045 VERSION BBa_K1813045.1 FEATURES Location/Qualifiers promoter 1..1319 /label=BBa_K1813019 promoter 1328..1367 /label=Ptac promoter 1334..1339 /label=-35 promoter 1356..1360 /label=-10 misc_feature 1363..1388 /label=lacO1 site promoter 1328..1388 /label=BBa_K864400 misc_feature 1401..1404 /label=conserved RBS 1397..1408 /label=BBa_B0034 CDS 1415..2812 /label=BBa_K1813000 stem_loop 2828..2847 /label=T7 TE misc_feature 2854..2854 /label=stop polyA_site 2848..2861 /label=polya misc_feature 2821..2861 /label=BBa_B0012 terminator 2821..2861 /label=BBa_B0012 stem_loop 2882..2904 /label=stem_loop misc_feature 2870..2915 /label=BBa_B0011 terminator 2870..2915 /label=BBa_B0011 terminator 2821..2915 /label=BBa_B0014 terminator 2821..2915 /label=BBa_K823017 misc_feature 1328..2915 /label=BBa_K1813001 promoter 2924..2963 /label=Ptac promoter 2930..2935 /label=-35 promoter 2952..2956 /label=-10 misc_feature 2959..2984 /label=lacO1 site promoter 2924..2984 /label=BBa_K864400 misc_feature 2997..3000 /label=conserved RBS 2993..3004 /label=BBa_B0034 CDS 3011..4162 /label=BBa_K1813006 stem_loop 4178..4197 /label=T7 TE misc_feature 4204..4204 /label=stop polyA_site 4198..4211 /label=polya misc_feature 4171..4211 /label=BBa_B0012 terminator 4171..4211 /label=BBa_B0012 stem_loop 4232..4254 /label=stem_loop misc_feature 4220..4265 /label=BBa_B0011 terminator 4220..4265 /label=BBa_B0011 terminator 4171..4265 /label=BBa_B0014 terminator 4171..4265 /label=BBa_K823017 misc_feature 2924..4265 /label=BBa_K1813014 misc_feature 1..4265 /label=BBa_K1813041 promoter 4274..4313 /label=Ptac promoter 4280..4285 /label=-35 promoter 4302..4306 /label=-10 misc_feature 4309..4334 /label=lacO1 site promoter 4274..4334 /label=BBa_K864400 misc_feature 4347..4350 /label=conserved RBS 4343..4354 /label=BBa_B0034 CDS 4361..5416 /label=BBa_K1813029 stem_loop 5432..5451 /label=T7 TE misc_feature 5458..5458 /label=stop polyA_site 5452..5465 /label=polya misc_feature 5425..5465 /label=BBa_B0012 terminator 5425..5465 /label=BBa_B0012 stem_loop 5486..5508 /label=stem_loop misc_feature 5474..5519 /label=BBa_B0011 terminator 5474..5519 /label=BBa_B0011 terminator 5425..5519 /label=BBa_B0014 terminator 5425..5519 /label=BBa_K823017 misc_feature 4274..5519 /label=BBa_K1813030 ORIGIN 1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa 61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac 121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga 301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg 421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg 481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg 541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc 601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc 661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat 721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg 781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc 841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc 901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac 961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac 1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc 1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt 1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa 1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag 1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat 1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga 1381 taacaattta ctagagaaag aggagaaata ctagatgtca ttgattgcaa ttaagaacgc 1441 tgactggatc ttaaccatgg atggcgcccg tcgcgtaatc gcagacggct ctattttaat 1501 cgataaagac cgtattcgcg ccatcggtaa aagtgctgtg atcgagattc ctccagagac 1561 aaccaaagtg attgacggtc gcggcaaagt ggttttgcca ggcttaattg acagtcatac 1621 ccaccactca ttacacttag gccgtggctt ggctgacgaa tgtgacattc agacattctt 1681 atatcgtcgc ttgtacccaa tcgaagccag tttaaatgac gaggatgcct atatctcagc 1741 attattatgc cagttagaaa tgattaaaag tggtaccacc tctatcatcg atgctggcaa 1801 ctactttcca gaggctacct tacgtgcatt tggcacaaca ggtatgcgtg gcgttgttgc 1861 acgttcaaca tttgacattc ctacttcatc tttgggttct ttacctgctc aggtgtttgc 1921 agaggagaca aatgtagcct taaaacgtgc cgaagaattt gtagagcgta actctggcgc 1981 atgcgacggc cgtgtgcagg catggttaca gttgcgcgtt ttacctaatt gtagtgatga 2041 gttgtgccgt ggtttgaaat ctattgctga ccgtttgggc gttcgctatg aagcacacgc 2101 cgcctttacc aaagaagtgt atgaagctag taaattacag ttcggcaaga gtgaagtgcg 2161 tcgtttggat gatttaggta tcttaggcga aggtttatta ttagctcata tgggttggtt 2221 aaccccacgc gatattttgt tattgattag ttcaaaaact aatgttgtat tatgcccaac 2281 ctctagtgta caccaggcta tgggttctat cgccttcggc cacgtgccag aattattaga 2341 aatgggtgtg aacgttgcat taggtactga tggtggtcct cacggcacaa atgacttggt 2401 acgccagatt ttcgtggccg caggcggcta taaagaagtt cgcttggatg ctacaatcat 2461 gcctccagaa acagttttag aaatggcaac cgttaatggt gcacgcgcca tgggtatgtc 2521 tgaccaggta ggctcaatcg agccaggtaa aaaagccgac attacaatct tcgactctcg 2581 ccgccctgaa tggcgcccat tacataatcc tgttgcaaat ttagtatatt gcgcaaacgg 2641 taattcagca gataccgtga ttgtagacgg ccgcatttta atggaaaacc gtaaaatctt 2701 aacctttaac gaggatgacg tgattaccga agcacagcgt cgctcagttg aaatcggtgc 2761 tcgtgctggc ttgttggaat acggtcgccc acgctggcca gttcattaat aatactagag 2821 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa 2881 aaagccagat tattaatccg gcttttttat tattttacta gaggagctgt tgacaattaa 2941 tcatcggctc gtataatgtg tggaattgtg agcggataac aatttactag agaaagagga 3001 gaaatactag atgcgcggtc gccagaagat cgcaattgtt ggtgctggct taggcggcgc 3061 agccgctgct actttattgc agcaagctgg ttttgatgtt gaagtgtttg agcaggcccc 3121 agcattcacc cgcttaggcg ctggcatcca catcggccct aacgttatga agatttttcg 3181 ccgtatgggt ttagagcaga aattagagtt aatgggctct cacccagact tctggttttc 3241 acgtgacggt aacaccggtg actacttgtc acgcattcca ttaggcgagt tcgcccgccg 3301 cgagtatggc gctgcctaca ttaccatcca ccgcggcgat ttacacgcat tgcagattga 3361 agcaattcag ccaggtaccg ttcactttgg caaacgttta gaaaaaattg ttgatgaagg 3421 tgaccaggtt cgtttagact tcgctgacgg tacccacacc gtggcagaca ttgtgatcgg 3481 cgcagacggc attcattcaa agattcgcga agagttattg ggtgccgaag ccccaatcta 3541 ttctggttgg gtggctcatc gtgcattgat tcgtggtgtg aatttagccc aacacgccga 3601 tgttttcgag ccatgcgtta agtggtggag tgaagaccgt catatgatgg tgtattatac 3661 tacaggcaag cgtgatgaat attatttcgt taccggcgtg cctcacgaag cctgggattt 3721 tcaaggcgca ttcgtggaca gttctcagga agagatgcgc gcagccttcg aaggttacca 3781 cccaacagtg caaaaattga tcgacgctac cgaatcaatc acaaaatggc ctttacgcaa 3841 ccgcaatcct ttacctttgt ggtcacgtgg ccgcttagtt ttattgggcg acgcttgtca 3901 tcctatgaaa cctcatatgg cacagggcgc ttgcatggct attgaggatg cagcaatgtt 3961 aacccgttgt ttacaggaaa ctggcttgag tgatcaccgc acagctttcg cattatacga 4021 agcaaaccgc aaggaacgcg cctcacaggt tcagagtgtt tctaatgcca acacttggtt 4081 atattctcag gaagatcctg cctgggtata tggttatgac ttgtatggtc agcagttaga 4141 aagtggcgaa gctgcttaat aatactagag tcacactggc tcaccttcgg gtgggccttt 4201 ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg gcttttttat 4261 tattttacta gaggagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg 4321 agcggataac aatttactag agaaagagga gaaatactag atgccagtaa gtaatgcaca 4381 gttaacccag atgttcgaac acgtgttaaa attaagtcgc gtggatgaaa ctcagtctgt 4441 tgcagtgtta aaaagtcatt atagtgaccc tcgcactgtg aacgcagcta tggaggcagc 4501 acaacgtttg aaagctaagg tttatgccgt tgaattacct gcatttaacc atccaaccgc 4561 tatgggtaat gatatgaccg cctattgcgg cgataccgca ttaactggta acttagccgc 4621 tcagcgtgct ttggaggcag cagacttggt tgtggacaca atgatgttat tgcacagtcc 4681 agaacaggag cagatcttga aaacaggcac tcgcatcttg ttggccgttg aaccacctga 4741 ggtgttggca cgcatgttac caaccgaaga tgacaagcgt cgtgtattgg ctgccgaaac 4801 cttgttgaaa caagcccgca gtttacatgt gcgttctaaa gccggcagtg attttcatgc 4861 accattgggt cagtatccag ccgtaaccga atatggttat gctgacgagc ctggccgttg 4921 ggatcattgg ccatctggct ttttatttac ttggccaaac gaggactcag cagaaggtac 4981 cttagtgttg gatgttggcg atattatctt gccattcaaa aactattgcc gcgaacgcat 5041 cactttggaa atcgaaaaag gcttcatcac cggtattcac ggtggctttg aagcagagta 5101 cttacgcgac tacatgaagt acttcaacga tcctgaggtt tatggcatta gtcacatcgg 5161 ttggggttta caaccacgtg cacaatggac tgccatgggc ttgcatgacc gcaatgacgg 5221 tatgtgtatg gatgctcgtg ccttttacgg caatttttta ttctctaccg gtccaaacac 5281 agaagtaggc ggtaagcgca agacaccatg ccacttggac atcccattgc gtaactgcga 5341 catttatttg gacgataaag ccgtagtatt ggctggtgac gtggtggcac cagaagaaag 5401 tcgcgcccgc taataatact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 5461 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattattt //