LOCUS BBa_K1813048 2797 bp DNA linear 15-Nov-2024 DEFINITION nicD, nicF and nicE Regulon ACCESSION BBa_K1813048 VERSION BBa_K1813048.1 FEATURES Location/Qualifiers promoter 1..40 /label=Ptac promoter 7..12 /label=-35 promoter 29..33 /label=-10 misc_feature 36..61 /label=lacO1 site promoter 1..61 /label=BBa_K864400 misc_feature 74..77 /label=conserved RBS 70..81 /label=BBa_B0034 CDS 88..897 /label=BBa_K1813007 stem_loop 913..932 /label=T7 TE misc_feature 939..939 /label=stop polyA_site 933..946 /label=polya misc_feature 906..946 /label=BBa_B0012 terminator 906..946 /label=BBa_B0012 stem_loop 967..989 /label=stem_loop misc_feature 955..1000 /label=BBa_B0011 terminator 955..1000 /label=BBa_B0011 terminator 906..1000 /label=BBa_B0014 terminator 906..1000 /label=BBa_K823017 misc_feature 1..1000 /label=BBa_K1813015 promoter 1009..1048 /label=Ptac promoter 1015..1020 /label=-35 promoter 1037..1041 /label=-10 misc_feature 1044..1069 /label=lacO1 site promoter 1009..1069 /label=BBa_K864400 misc_feature 1082..1085 /label=conserved RBS 1078..1089 /label=BBa_B0034 CDS 1096..1740 /label=BBa_K1813009 stem_loop 1756..1775 /label=T7 TE misc_feature 1782..1782 /label=stop polyA_site 1776..1789 /label=polya misc_feature 1749..1789 /label=BBa_B0012 terminator 1749..1789 /label=BBa_B0012 stem_loop 1810..1832 /label=stem_loop misc_feature 1798..1843 /label=BBa_B0011 terminator 1798..1843 /label=BBa_B0011 terminator 1749..1843 /label=BBa_B0014 terminator 1749..1843 /label=BBa_K823017 misc_feature 1009..1843 /label=BBa_K1813017 promoter 1852..1891 /label=Ptac promoter 1858..1863 /label=-35 promoter 1880..1884 /label=-10 misc_feature 1887..1912 /label=lacO1 site promoter 1852..1912 /label=BBa_K864400 misc_feature 1925..1928 /label=conserved RBS 1921..1932 /label=BBa_B0034 CDS 1939..2694 /label=BBa_K1813008 stem_loop 2710..2729 /label=T7 TE misc_feature 2736..2736 /label=stop polyA_site 2730..2743 /label=polya misc_feature 2703..2743 /label=BBa_B0012 terminator 2703..2743 /label=BBa_B0012 stem_loop 2764..2786 /label=stem_loop misc_feature 2752..2797 /label=BBa_B0011 terminator 2752..2797 /label=BBa_B0011 terminator 2703..2797 /label=BBa_B0014 terminator 2703..2797 /label=BBa_K823017 misc_feature 1852..2797 /label=BBa_K1813016 misc_feature 1009..2797 /label=BBa_K1813044 ORIGIN 1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat 61 ttactagaga aagaggagaa atactagatg agtacctttg tggcaggcgg caacgtatct 121 gccaacggca ttcgtcaaca ctatttgcgt tatggcggca aaggtcacgc tttaatctta 181 gttccaggca ttacatctcc tgccatcaca tggggcttcg tggctgaacg cttaggtcat 241 tactttgaca cctatgtatt ggacgttcgt ggtcgtggct tatctagttc aggccctgac 301 ttggattacg gcactgacgc atgcgccgcc gacatcccag cttttgccgc agccttgggt 361 ttggatagtt accacttatt aggtcattct atgggcgccc gttttgcaat ccgcgccgcc 421 gcacaaggcg ccccaggctt gcagcgctta gtgttagtag accctccagt aagtggtcca 481 ggccgtcgtg catacccatc aaaattacct tggtacgtgg attctattcg ccaagctacc 541 gtaggcatga gtggtgacga tatgcgcgcc ttctgtgcta cttggtcaga tgaacagtta 601 gctttacgcg cagagtggtt acacacatgt tatgaaccag ccatcgtgcg tgcatttgac 661 gattttcacg aagttgatat tcaccagtat ttacctgctg tgcgccagcc tgccttatta 721 atggttgccg gtcgtggcgg cgttattgaa ccacgtgata ttgccgaaat gcgtgagttg 781 aaaccagaca tccaagtggc ctacgttgat aatgccggcc acatgatccc atgggatgat 841 ttagacggtt tcttcgccgc attcggcgat ttcttagatc acccattggt ttaataatac 901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa 961 tataaaaagc cagattatta atccggcttt tttattattt tactagagga gctgttgaca 1021 attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt actagagaaa 1081 gaggagaaat actagatgag tgatgctcaa tcagcacgtg ataattatca gggcgtgtgg 1141 ggccagcgta tcggttttgg ccgtaaacca gcattattaa tgatcgactt catgcagggc 1201 tacacaaccc ctggcgcacc tttatacgct cctggtgtag ttgcagcagt ggagcaagca 1261 gcaggtttgt tggctttggc ccgcgactgt ggcacattag tggtgcatac aaacattcgc 1321 tatcagcctc cacatttcgc cgatggtggt gtgtgggttc gtaaggcccc tgtgatgaaa 1381 gacatggttg aaggcaaccc tttagctgcc ttctgtgaag ccgtagcacc tcaggcaggc 1441 gaagtagtat tgtctaaaca gtatgcaagt gccttttttg ccacttcatt ggccccatta 1501 ttacatgccc agggtgttga tactgtggta ttggccggct gtagtacctc tggttgcatt 1561 cgtgcaagtg ctgtggatgc tatgcagcac ggcttccgca caatcgtagt gcgcgaatgc 1621 gtaggtgacc gccatagtga cccacatgaa gcaaacttat tcgatattga cagtaaatat 1681 ggcgatgtgg ttacccgtca agatgctatg cagcagttac gccacttagc aggctaataa 1741 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagaga 1801 gaatataaaa agccagatta ttaatccggc ttttttatta ttttactaga ggagctgttg 1861 acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttactagag 1921 aaagaggaga aatactagat gacccagtta tatcgtatcg gtcagattgt acctagttct 1981 aataccacaa tggagaccga aattcctgct atgttgaatg cccgtcaggc tatccgccct 2041 gaacgtttta cttttcactc atctcgcatg cgcatgaaac aagtaaaaaa agaagaatta 2101 gctgctatgg atgcagagag tgaccgctgc gcagttgaat tgtctgatgc aaaagtggat 2161 gtattaggtt atgcatgctt agtagcaatt atggcaatgg gcttaggcta ccatcgccag 2221 tcagagaaac gcttacagca agcaaccgcc gataacgatg cattggcccc tgtgatcact 2281 tcagcaggcg ccttagtgga agcattacat gtgatgaagg ccaaacgcat cgccattgta 2341 gcaccatata tgaagccatt gaccgaatta gtggttaact acatccgtga agaaggcttc 2401 gaggttcagg actggcgtgc attagaaatc cctgataatt tggccgtagc ccgccatgat 2461 ccagctaact tgcctggtat tgtggcaggt atggatttgg aaggtgttga tgtagttgtt 2521 ttatctgcat gtgtgcaaat gcagtcatta cctgccgtgg ccaaggttga agcccaaacc 2581 ggtaaacctg ttgtgacagc cgccatcgca accacctacg ccatgttgaa agccttagat 2641 ttagagccta ttgtacctgg cgcaggcgct ttgttatctg gtgcttacta ataatactag 2701 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat 2761 aaaaagccag attattaatc cggctttttt attattt //