##gff-version 3
##sequence-region BBa_K1813048 1 2797
BBa_K1813048	.	engineered_region	1	1000	.	+	0	ID=BBa_K1813015;Name=BBa_K1813015
BBa_K1813048	.	terminator	906	1000	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813015
BBa_K1813048	.	terminator	906	1000	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813048	.	terminator	955	1000	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813048	.	stem_loop	967	989	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813048	.	engineered_region	955	1000	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813048	.	terminator	906	946	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813048	.	stem_loop	913	932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813048	.	stop_codon	939	939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813048	.	engineered_region	906	946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813048	.	polyA_site	933	946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813048	.	promoter	1	61	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813015
BBa_K1813048	.	promoter	7	12	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
BBa_K1813048	.	promoter	1	40	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813048	.	operator	36	61	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813048	.	promoter	29	33	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813048	.	ribosome_entry_site	70	81	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813015
BBa_K1813048	.	conserved_region	74	77	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1813048	.	CDS	88	897	.	+	0	ID=BBa_K1813007;Name=BBa_K1813007;Parent=BBa_K1813015
BBa_K1813048	.	engineered_region	1009	2797	.	+	0	ID=BBa_K1813044;Name=BBa_K1813044
BBa_K1813048	.	engineered_region	1009	1843	.	+	0	ID=BBa_K1813017;Name=BBa_K1813017;Parent=BBa_K1813044
BBa_K1813048	.	terminator	1749	1843	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813017
BBa_K1813048	.	terminator	1749	1843	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813048	.	terminator	1798	1843	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813048	.	stem_loop	1810	1832	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813048	.	engineered_region	1798	1843	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813048	.	terminator	1749	1789	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813048	.	stem_loop	1756	1775	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813048	.	stop_codon	1782	1782	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813048	.	engineered_region	1749	1789	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813048	.	polyA_site	1776	1789	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813048	.	promoter	1009	1069	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813017
BBa_K1813048	.	promoter	1015	1020	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
BBa_K1813048	.	promoter	1009	1048	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813048	.	operator	1044	1069	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813048	.	promoter	1037	1041	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813048	.	ribosome_entry_site	1078	1089	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813017
BBa_K1813048	.	conserved_region	1082	1085	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1813048	.	CDS	1096	1740	.	+	0	ID=BBa_K1813009;Name=BBa_K1813009;Parent=BBa_K1813017
BBa_K1813048	.	engineered_region	1852	2797	.	+	0	ID=BBa_K1813016;Name=BBa_K1813016;Parent=BBa_K1813044
BBa_K1813048	.	terminator	2703	2797	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813016
BBa_K1813048	.	terminator	2703	2797	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813048	.	terminator	2752	2797	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813048	.	stem_loop	2764	2786	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813048	.	engineered_region	2752	2797	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813048	.	terminator	2703	2743	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813048	.	stem_loop	2710	2729	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813048	.	stop_codon	2736	2736	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813048	.	engineered_region	2703	2743	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813048	.	polyA_site	2730	2743	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813048	.	CDS	1939	2694	.	+	0	ID=BBa_K1813008;Name=BBa_K1813008;Parent=BBa_K1813016
BBa_K1813048	.	promoter	1852	1912	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813016
BBa_K1813048	.	promoter	1858	1863	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
BBa_K1813048	.	promoter	1852	1891	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813048	.	operator	1887	1912	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813048	.	promoter	1880	1884	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813048	.	ribosome_entry_site	1921	1932	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813016
BBa_K1813048	.	conserved_region	1925	1928	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1813048
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggaga
aatactagatgagtacctttgtggcaggcggcaacgtatctgccaacggcattcgtcaacactatttgcgttatggcgg
caaaggtcacgctttaatcttagttccaggcattacatctcctgccatcacatggggcttcgtggctgaacgcttaggt
cattactttgacacctatgtattggacgttcgtggtcgtggcttatctagttcaggccctgacttggattacggcactg
acgcatgcgccgccgacatcccagcttttgccgcagccttgggtttggatagttaccacttattaggtcattctatggg
cgcccgttttgcaatccgcgccgccgcacaaggcgccccaggcttgcagcgcttagtgttagtagaccctccagtaagt
ggtccaggccgtcgtgcatacccatcaaaattaccttggtacgtggattctattcgccaagctaccgtaggcatgagtg
gtgacgatatgcgcgccttctgtgctacttggtcagatgaacagttagctttacgcgcagagtggttacacacatgtta
tgaaccagccatcgtgcgtgcatttgacgattttcacgaagttgatattcaccagtatttacctgctgtgcgccagcct
gccttattaatggttgccggtcgtggcggcgttattgaaccacgtgatattgccgaaatgcgtgagttgaaaccagaca
tccaagtggcctacgttgataatgccggccacatgatcccatgggatgatttagacggtttcttcgccgcattcggcga
tttcttagatcacccattggtttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggagctgttgacaattaatc
atcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtgatgc
tcaatcagcacgtgataattatcagggcgtgtggggccagcgtatcggttttggccgtaaaccagcattattaatgatc
gacttcatgcagggctacacaacccctggcgcacctttatacgctcctggtgtagttgcagcagtggagcaagcagcag
gtttgttggctttggcccgcgactgtggcacattagtggtgcatacaaacattcgctatcagcctccacatttcgccga
tggtggtgtgtgggttcgtaaggcccctgtgatgaaagacatggttgaaggcaaccctttagctgccttctgtgaagcc
gtagcacctcaggcaggcgaagtagtattgtctaaacagtatgcaagtgccttttttgccacttcattggccccattat
tacatgcccagggtgttgatactgtggtattggccggctgtagtacctctggttgcattcgtgcaagtgctgtggatgc
tatgcagcacggcttccgcacaatcgtagtgcgcgaatgcgtaggtgaccgccatagtgacccacatgaagcaaactta
ttcgatattgacagtaaatatggcgatgtggttacccgtcaagatgctatgcagcagttacgccacttagcaggctaat
aatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccaga
ttattaatccggcttttttattattttactagaggagctgttgacaattaatcatcggctcgtataatgtgtggaattg
tgagcggataacaatttactagagaaagaggagaaatactagatgacccagttatatcgtatcggtcagattgtaccta
gttctaataccacaatggagaccgaaattcctgctatgttgaatgcccgtcaggctatccgccctgaacgttttacttt
tcactcatctcgcatgcgcatgaaacaagtaaaaaaagaagaattagctgctatggatgcagagagtgaccgctgcgca
gttgaattgtctgatgcaaaagtggatgtattaggttatgcatgcttagtagcaattatggcaatgggcttaggctacc
atcgccagtcagagaaacgcttacagcaagcaaccgccgataacgatgcattggcccctgtgatcacttcagcaggcgc
cttagtggaagcattacatgtgatgaaggccaaacgcatcgccattgtagcaccatatatgaagccattgaccgaatta
gtggttaactacatccgtgaagaaggcttcgaggttcaggactggcgtgcattagaaatccctgataatttggccgtag
cccgccatgatccagctaacttgcctggtattgtggcaggtatggatttggaaggtgttgatgtagttgttttatctgc
atgtgtgcaaatgcagtcattacctgccgtggccaaggttgaagcccaaaccggtaaacctgttgtgacagccgccatc
gcaaccacctacgccatgttgaaagccttagatttagagcctattgtacctggcgcaggcgctttgttatctggtgctt
actaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaa
gccagattattaatccggcttttttattattt