BBa_K864400 1 BBa_K864400 Ptac, trp & lac regulated promoter 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG Released HQ 2013 The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]] [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25 false false _1124_ 0 9827 9 In stock false Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone. false Erik Lundin annotation2197240 1 lacO1 site range2197240 1 36 61 annotation2197238 1 -10 range2197238 1 29 33 annotation2197237 1 -35 range2197237 1 7 12 annotation2197236 1 Ptac range2197236 1 1 40 BBa_K1813048 1 BBa_K1813048 <i>nicD, nicF and nicE</i> Regulon 2015-09-10T11:00:00Z 2015-09-18T07:45:11Z Registry and Synthesized ptac nicD nicF nicE false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 component2449779 1 BBa_K1813015 component2449814 1 BBa_K1813044 annotation2449779 1 BBa_K1813015 range2449779 1 1 1000 annotation2449814 1 BBa_K1813044 range2449814 1 1009 2797 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_K823017 1 BBa_K823017 double terminator (B0012-B0011) 2012-09-10T11:00:00Z 2015-05-08T01:13:30Z Part:BBa_B0014 Released HQ 2013 This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3. false false _1081_ 0 11555 9 In stock false This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3. false Jara Radeck component2182657 1 BBa_B0014 annotation2182657 1 BBa_B0014 range2182657 1 1 95 BBa_K1813044 1 BBa_K1813044 <i>nicF and nicE</i> Expression Cassette 2015-09-10T11:00:00Z 2015-09-18T09:52:45Z Registry and synthesized ptac nicF nicE false false _2238_ 12892 12892 9 false NA false UBC iGEM 2015 component2449621 1 BBa_K1813017 component2449638 1 BBa_K1813016 annotation2449621 1 BBa_K1813017 range2449621 1 1 835 annotation2449638 1 BBa_K1813016 range2449638 1 844 1789 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_K1813016 1 BBa_K1813016 <i>nicE</i> Expression Casette 2015-07-26T11:00:00Z 2015-09-18T04:10:09Z Synthesized and registry ptac nicE term false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 component2433646 1 BBa_K864400 component2433649 1 BBa_K1813008 component2433657 1 BBa_K823017 component2433648 1 BBa_B0034 annotation2433648 1 BBa_B0034 range2433648 1 70 81 annotation2433649 1 BBa_K1813008 range2433649 1 88 843 annotation2433657 1 BBa_K823017 range2433657 1 852 946 annotation2433646 1 BBa_K864400 range2433646 1 1 61 BBa_K1813007 1 BBa_K1813007 <i>nicD</i> 2015-07-26T11:00:00Z 2015-09-18T11:13:27Z Synthesized nicD false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1813017 1 BBa_K1813017 <i>nicF</i> Expression Cassette 2015-07-26T11:00:00Z 2015-09-18T04:10:44Z Synthesized and registry ptac nicF term false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 component2433664 1 BBa_B0034 component2433662 1 BBa_K864400 component2433673 1 BBa_K823017 component2433665 1 BBa_K1813009 annotation2433662 1 BBa_K864400 range2433662 1 1 61 annotation2433673 1 BBa_K823017 range2433673 1 741 835 annotation2433664 1 BBa_B0034 range2433664 1 70 81 annotation2433665 1 BBa_K1813009 range2433665 1 88 732 BBa_K1813015 1 BBa_K1813015 <i>nicD</i> Expression Casette 2015-07-26T11:00:00Z 2015-09-18T04:09:51Z Synthesized and registry ptac nicD term false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 component2433641 1 BBa_K823017 component2433633 1 BBa_K1813007 component2433630 1 BBa_K864400 component2433632 1 BBa_B0034 annotation2433633 1 BBa_K1813007 range2433633 1 88 897 annotation2433632 1 BBa_B0034 range2433632 1 70 81 annotation2433630 1 BBa_K864400 range2433630 1 1 61 annotation2433641 1 BBa_K823017 range2433641 1 906 1000 BBa_K1813009 1 BBa_K1813009 <i>nicF</i> 2015-07-26T11:00:00Z 2015-09-18T11:14:06Z Synthesized nicF false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 BBa_K1813008 1 BBa_K1813008 <i>nicE</i> 2015-07-26T11:00:00Z 2015-09-18T11:13:45Z Synthesized nicE false false _2238_ 25865 12892 9 false NA false UBC iGEM 2015 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813007_sequence 1 atgagtacctttgtggcaggcggcaacgtatctgccaacggcattcgtcaacactatttgcgttatggcggcaaaggtcacgctttaatcttagttccaggcattacatctcctgccatcacatggggcttcgtggctgaacgcttaggtcattactttgacacctatgtattggacgttcgtggtcgtggcttatctagttcaggccctgacttggattacggcactgacgcatgcgccgccgacatcccagcttttgccgcagccttgggtttggatagttaccacttattaggtcattctatgggcgcccgttttgcaatccgcgccgccgcacaaggcgccccaggcttgcagcgcttagtgttagtagaccctccagtaagtggtccaggccgtcgtgcatacccatcaaaattaccttggtacgtggattctattcgccaagctaccgtaggcatgagtggtgacgatatgcgcgccttctgtgctacttggtcagatgaacagttagctttacgcgcagagtggttacacacatgttatgaaccagccatcgtgcgtgcatttgacgattttcacgaagttgatattcaccagtatttacctgctgtgcgccagcctgccttattaatggttgccggtcgtggcggcgttattgaaccacgtgatattgccgaaatgcgtgagttgaaaccagacatccaagtggcctacgttgataatgccggccacatgatcccatgggatgatttagacggtttcttcgccgcattcggcgatttcttagatcacccattggtttaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1813017_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtgatgctcaatcagcacgtgataattatcagggcgtgtggggccagcgtatcggttttggccgtaaaccagcattattaatgatcgacttcatgcagggctacacaacccctggcgcacctttatacgctcctggtgtagttgcagcagtggagcaagcagcaggtttgttggctttggcccgcgactgtggcacattagtggtgcatacaaacattcgctatcagcctccacatttcgccgatggtggtgtgtgggttcgtaaggcccctgtgatgaaagacatggttgaaggcaaccctttagctgccttctgtgaagccgtagcacctcaggcaggcgaagtagtattgtctaaacagtatgcaagtgccttttttgccacttcattggccccattattacatgcccagggtgttgatactgtggtattggccggctgtagtacctctggttgcattcgtgcaagtgctgtggatgctatgcagcacggcttccgcacaatcgtagtgcgcgaatgcgtaggtgaccgccatagtgacccacatgaagcaaacttattcgatattgacagtaaatatggcgatgtggttacccgtcaagatgctatgcagcagttacgccacttagcaggctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813008_sequence 1 atgacccagttatatcgtatcggtcagattgtacctagttctaataccacaatggagaccgaaattcctgctatgttgaatgcccgtcaggctatccgccctgaacgttttacttttcactcatctcgcatgcgcatgaaacaagtaaaaaaagaagaattagctgctatggatgcagagagtgaccgctgcgcagttgaattgtctgatgcaaaagtggatgtattaggttatgcatgcttagtagcaattatggcaatgggcttaggctaccatcgccagtcagagaaacgcttacagcaagcaaccgccgataacgatgcattggcccctgtgatcacttcagcaggcgccttagtggaagcattacatgtgatgaaggccaaacgcatcgccattgtagcaccatatatgaagccattgaccgaattagtggttaactacatccgtgaagaaggcttcgaggttcaggactggcgtgcattagaaatccctgataatttggccgtagcccgccatgatccagctaacttgcctggtattgtggcaggtatggatttggaaggtgttgatgtagttgttttatctgcatgtgtgcaaatgcagtcattacctgccgtggccaaggttgaagcccaaaccggtaaacctgttgtgacagccgccatcgcaaccacctacgccatgttgaaagccttagatttagagcctattgtacctggcgcaggcgctttgttatctggtgcttactaataa BBa_K823017_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813044_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtgatgctcaatcagcacgtgataattatcagggcgtgtggggccagcgtatcggttttggccgtaaaccagcattattaatgatcgacttcatgcagggctacacaacccctggcgcacctttatacgctcctggtgtagttgcagcagtggagcaagcagcaggtttgttggctttggcccgcgactgtggcacattagtggtgcatacaaacattcgctatcagcctccacatttcgccgatggtggtgtgtgggttcgtaaggcccctgtgatgaaagacatggttgaaggcaaccctttagctgccttctgtgaagccgtagcacctcaggcaggcgaagtagtattgtctaaacagtatgcaagtgccttttttgccacttcattggccccattattacatgcccagggtgttgatactgtggtattggccggctgtagtacctctggttgcattcgtgcaagtgctgtggatgctatgcagcacggcttccgcacaatcgtagtgcgcgaatgcgtaggtgaccgccatagtgacccacatgaagcaaacttattcgatattgacagtaaatatggcgatgtggttacccgtcaagatgctatgcagcagttacgccacttagcaggctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgacccagttatatcgtatcggtcagattgtacctagttctaataccacaatggagaccgaaattcctgctatgttgaatgcccgtcaggctatccgccctgaacgttttacttttcactcatctcgcatgcgcatgaaacaagtaaaaaaagaagaattagctgctatggatgcagagagtgaccgctgcgcagttgaattgtctgatgcaaaagtggatgtattaggttatgcatgcttagtagcaattatggcaatgggcttaggctaccatcgccagtcagagaaacgcttacagcaagcaaccgccgataacgatgcattggcccctgtgatcacttcagcaggcgccttagtggaagcattacatgtgatgaaggccaaacgcatcgccattgtagcaccatatatgaagccattgaccgaattagtggttaactacatccgtgaagaaggcttcgaggttcaggactggcgtgcattagaaatccctgataatttggccgtagcccgccatgatccagctaacttgcctggtattgtggcaggtatggatttggaaggtgttgatgtagttgttttatctgcatgtgtgcaaatgcagtcattacctgccgtggccaaggttgaagcccaaaccggtaaacctgttgtgacagccgccatcgcaaccacctacgccatgttgaaagccttagatttagagcctattgtacctggcgcaggcgctttgttatctggtgcttactaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813016_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgacccagttatatcgtatcggtcagattgtacctagttctaataccacaatggagaccgaaattcctgctatgttgaatgcccgtcaggctatccgccctgaacgttttacttttcactcatctcgcatgcgcatgaaacaagtaaaaaaagaagaattagctgctatggatgcagagagtgaccgctgcgcagttgaattgtctgatgcaaaagtggatgtattaggttatgcatgcttagtagcaattatggcaatgggcttaggctaccatcgccagtcagagaaacgcttacagcaagcaaccgccgataacgatgcattggcccctgtgatcacttcagcaggcgccttagtggaagcattacatgtgatgaaggccaaacgcatcgccattgtagcaccatatatgaagccattgaccgaattagtggttaactacatccgtgaagaaggcttcgaggttcaggactggcgtgcattagaaatccctgataatttggccgtagcccgccatgatccagctaacttgcctggtattgtggcaggtatggatttggaaggtgttgatgtagttgttttatctgcatgtgtgcaaatgcagtcattacctgccgtggccaaggttgaagcccaaaccggtaaacctgttgtgacagccgccatcgcaaccacctacgccatgttgaaagccttagatttagagcctattgtacctggcgcaggcgctttgttatctggtgcttactaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813009_sequence 1 atgagtgatgctcaatcagcacgtgataattatcagggcgtgtggggccagcgtatcggttttggccgtaaaccagcattattaatgatcgacttcatgcagggctacacaacccctggcgcacctttatacgctcctggtgtagttgcagcagtggagcaagcagcaggtttgttggctttggcccgcgactgtggcacattagtggtgcatacaaacattcgctatcagcctccacatttcgccgatggtggtgtgtgggttcgtaaggcccctgtgatgaaagacatggttgaaggcaaccctttagctgccttctgtgaagccgtagcacctcaggcaggcgaagtagtattgtctaaacagtatgcaagtgccttttttgccacttcattggccccattattacatgcccagggtgttgatactgtggtattggccggctgtagtacctctggttgcattcgtgcaagtgctgtggatgctatgcagcacggcttccgcacaatcgtagtgcgcgaatgcgtaggtgaccgccatagtgacccacatgaagcaaacttattcgatattgacagtaaatatggcgatgtggttacccgtcaagatgctatgcagcagttacgccacttagcaggctaataa BBa_K1813048_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtacctttgtggcaggcggcaacgtatctgccaacggcattcgtcaacactatttgcgttatggcggcaaaggtcacgctttaatcttagttccaggcattacatctcctgccatcacatggggcttcgtggctgaacgcttaggtcattactttgacacctatgtattggacgttcgtggtcgtggcttatctagttcaggccctgacttggattacggcactgacgcatgcgccgccgacatcccagcttttgccgcagccttgggtttggatagttaccacttattaggtcattctatgggcgcccgttttgcaatccgcgccgccgcacaaggcgccccaggcttgcagcgcttagtgttagtagaccctccagtaagtggtccaggccgtcgtgcatacccatcaaaattaccttggtacgtggattctattcgccaagctaccgtaggcatgagtggtgacgatatgcgcgccttctgtgctacttggtcagatgaacagttagctttacgcgcagagtggttacacacatgttatgaaccagccatcgtgcgtgcatttgacgattttcacgaagttgatattcaccagtatttacctgctgtgcgccagcctgccttattaatggttgccggtcgtggcggcgttattgaaccacgtgatattgccgaaatgcgtgagttgaaaccagacatccaagtggcctacgttgataatgccggccacatgatcccatgggatgatttagacggtttcttcgccgcattcggcgatttcttagatcacccattggtttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtgatgctcaatcagcacgtgataattatcagggcgtgtggggccagcgtatcggttttggccgtaaaccagcattattaatgatcgacttcatgcagggctacacaacccctggcgcacctttatacgctcctggtgtagttgcagcagtggagcaagcagcaggtttgttggctttggcccgcgactgtggcacattagtggtgcatacaaacattcgctatcagcctccacatttcgccgatggtggtgtgtgggttcgtaaggcccctgtgatgaaagacatggttgaaggcaaccctttagctgccttctgtgaagccgtagcacctcaggcaggcgaagtagtattgtctaaacagtatgcaagtgccttttttgccacttcattggccccattattacatgcccagggtgttgatactgtggtattggccggctgtagtacctctggttgcattcgtgcaagtgctgtggatgctatgcagcacggcttccgcacaatcgtagtgcgcgaatgcgtaggtgaccgccatagtgacccacatgaagcaaacttattcgatattgacagtaaatatggcgatgtggttacccgtcaagatgctatgcagcagttacgccacttagcaggctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagaggagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgacccagttatatcgtatcggtcagattgtacctagttctaataccacaatggagaccgaaattcctgctatgttgaatgcccgtcaggctatccgccctgaacgttttacttttcactcatctcgcatgcgcatgaaacaagtaaaaaaagaagaattagctgctatggatgcagagagtgaccgctgcgcagttgaattgtctgatgcaaaagtggatgtattaggttatgcatgcttagtagcaattatggcaatgggcttaggctaccatcgccagtcagagaaacgcttacagcaagcaaccgccgataacgatgcattggcccctgtgatcacttcagcaggcgccttagtggaagcattacatgtgatgaaggccaaacgcatcgccattgtagcaccatatatgaagccattgaccgaattagtggttaactacatccgtgaagaaggcttcgaggttcaggactggcgtgcattagaaatccctgataatttggccgtagcccgccatgatccagctaacttgcctggtattgtggcaggtatggatttggaaggtgttgatgtagttgttttatctgcatgtgtgcaaatgcagtcattacctgccgtggccaaggttgaagcccaaaccggtaaacctgttgtgacagccgccatcgcaaccacctacgccatgttgaaagccttagatttagagcctattgtacctggcgcaggcgctttgttatctggtgcttactaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1813015_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgagtacctttgtggcaggcggcaacgtatctgccaacggcattcgtcaacactatttgcgttatggcggcaaaggtcacgctttaatcttagttccaggcattacatctcctgccatcacatggggcttcgtggctgaacgcttaggtcattactttgacacctatgtattggacgttcgtggtcgtggcttatctagttcaggccctgacttggattacggcactgacgcatgcgccgccgacatcccagcttttgccgcagccttgggtttggatagttaccacttattaggtcattctatgggcgcccgttttgcaatccgcgccgccgcacaaggcgccccaggcttgcagcgcttagtgttagtagaccctccagtaagtggtccaggccgtcgtgcatacccatcaaaattaccttggtacgtggattctattcgccaagctaccgtaggcatgagtggtgacgatatgcgcgccttctgtgctacttggtcagatgaacagttagctttacgcgcagagtggttacacacatgttatgaaccagccatcgtgcgtgcatttgacgattttcacgaagttgatattcaccagtatttacctgctgtgcgccagcctgccttattaatggttgccggtcgtggcggcgttattgaaccacgtgatattgccgaaatgcgtgagttgaaaccagacatccaagtggcctacgttgataatgccggccacatgatcccatgggatgatttagacggtttcttcgccgcattcggcgatttcttagatcacccattggtttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K864400_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z