LOCUS       BBa_K1813054            2299 bp    DNA     linear       22-Dec-2024
DEFINITION  pelB <i>AgCPR</i>  Expression Cassette
ACCESSION   BBa_K1813054
VERSION     BBa_K1813054.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     misc_feature    1..81
                     /label=BBa_K1813037
     misc_feature    88..153
                     /label=pelB
     CDS             88..2196
                     /label=BBa_K1813053
     stem_loop       2212..2231
                     /label=T7 TE
     misc_feature    2238..2238
                     /label=stop
     polyA_site      2232..2245
                     /label=polya
     misc_feature    2205..2245
                     /label=BBa_B0012
     terminator      2205..2245
                     /label=BBa_B0012
     stem_loop       2266..2288
                     /label=stem_loop
     misc_feature    2254..2299
                     /label=BBa_B0011
     terminator      2254..2299
                     /label=BBa_B0011
     terminator      2205..2299
                     /label=BBa_B0014
     terminator      2205..2299
                     /label=BBa_K823017
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg aaatacctgc tgccgaccgc tgctgctggt
      121 ctgctgctcc tcgctgccca gccggcgatg gccatggacg ctcagaccga aaccgaagtg
      181 ccagcaggct ctgtgagtga tgaacctttc ttaggtcctt tagacatcgt tttattggta
      241 tctttattgg ccggtacagc atggtatttg ttgaaaggca agaaaaaaga aagtcaggca
      301 tcacagttta aatcatatag tattcaacca acaaccgtga atacaatgac catggtggaa
      361 aacagtttca tcaagaaatt acagagttct ggtcgtcgct tagttgtttt ttatggttca
      421 cagaccggta cagcagaaga atttgccggt cgcttagcta aagaaggcat ccgttatcag
      481 atgaaaggta tggtagcaga tccagaagaa tgtaacatgg aagagttgtt aatgttaaaa
      541 gacatcgaca agtcattggc tgtgttttgc ttagcaacct atggtgaagg tgatccaacc
      601 gataactgta tggaatttta tgattggatc cagaacaatg atttagatat gactggcttg
      661 aattacgctg tgtttggttt gggtaataaa acctacgagc attataataa agttggcatt
      721 tacgttgata aacgcttgga agaattgggt gcaaaccgtg tttttgaatt gggcttgggc
      781 gatgacgatg ccaacattga agattatttc atcacctgga aagaaaagtt ttggccaaca
      841 gtttgcgatt atttcggtat tgaaagtaca ggcgaagacg ttttgatgcg tcagtatcgc
      901 ttattggagc aaccagatgt gtcagccgat cgcatttaca ccggcgaggt ggcacgctta
      961 cacagtttgc aaacccaacg cccacctttt gacgccaaaa accctttctt ggctccaatc
     1021 aaagttaatc gtgagttgca taaagcaggt ggccgtagtt gcatgcatgt ggaatttgat
     1081 atcgaaggta gtaaaatgcg ttatgaggct ggtgaccatt tagccatgta tcctgttaac
     1141 gatcgcgatt tggttgagcg tttaggtcgt ttatgtaacg ctgaattgga caccgttttc
     1201 tctttaatca acacagacac agattcttca aaaaaacatc cattcccttg cccaaccact
     1261 taccgtactg ccttaaccca ctacttagaa atcacagcct tgccacgtac ccacattttg
     1321 aaagaattag ctgaatattg cggtgaggaa aaagataaag aatttttgcg ttttatctct
     1381 tctaccgcac cagatggcaa ggcaaagtat caggagtggg ttcaggatag ttgtcgcaac
     1441 atcgtacacg tgttggaaga cattccttct tgccaccctc ctattgacca cgtgtgtgag
     1501 ttattaccac gtttacaacc tcgctaccac tctatttctt ctagttcaaa attacaccct
     1561 accacagttc atgtgaccgc agtattagta aaatatgaaa ccaaaaccgg tcgcttaaat
     1621 aaaggtgtag ctaccacttt cttggccgag aaacatccaa atgatggcga accagcccca
     1681 cgtgttccaa tttttatccg caaatctcag tttcgtttgc ctccaaaacc agaaacccca
     1741 gtgattatgg tgggcccagg taccggtttg gctcctttcc gtggttttat tcaggagcgc
     1801 gaccattgca aacaggaggg caaagaaatt ggtcagacca ctttatattt tggctgtcgc
     1861 aaacgtagtg aagactatat ctatgaagac gaattggagg actacagtaa acgcggcatc
     1921 atcaatttac gtgtggcctt ttcacgcgat caagagaaaa aagtgtatgt gactcactta
     1981 ttagaacagg actctgattt gatttggagt gtgatcggtg aaaataaggg tcatttctat
     2041 atttgcggtg acgccaaaaa catggctact gatgtgcgca acatcttgtt gaaagtaatt
     2101 cgtagtaaag gcggcttatc agagaccgaa gctcaacaat acatcaagaa gatggaggca
     2161 cagaagcgtt attctgctga tgtatggtca taataatact agagtcacac tggctcacct
     2221 tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa
     2281 tccggctttt ttattattt
//