BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1813056
1
BBa_K1813056
ptac pelB CYP2D6
2015-09-10T11:00:00Z
2015-09-18T06:56:26Z
Synthesized and registry
ptac pelB HUMCYPDB1
false
false
_2238_
19256
12892
9
false
NA
false
UBC iGEM 2015
component2449944
1
BBa_K823017
component2449934
1
BBa_K1813037
component2449936
1
BBa_K1813055
annotation2449934
1
BBa_K1813037
range2449934
1
1
81
annotation2449944
1
BBa_K823017
range2449944
1
1659
1753
annotation2449936
1
BBa_K1813055
range2449936
1
88
1650
BBa_K1813055
1
BBa_K1813055
pelB CYP2D6
2015-09-10T11:00:00Z
2015-09-18T06:56:40Z
Synthesized
pelB HUMCYPDB1
false
false
_2238_
19256
12892
9
false
NA
false
UBC iGEM 2015
annotation2449926
1
pelB
range2449926
1
1
66
BBa_K1813037
1
BBa_K1813037
ptac and RBS
2015-09-10T11:00:00Z
2015-09-18T07:18:03Z
Registry
ptac RBS
false
false
_2238_
25865
12892
9
false
NA
false
UBC iGEM 2015
component2449457
1
BBa_K864400
component2449459
1
BBa_B0034
annotation2449457
1
BBa_K864400
range2449457
1
1
61
annotation2449459
1
BBa_B0034
range2449459
1
70
81
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939311
1
BBa_B0011
component939303
1
BBa_B0012
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_K864400
1
BBa_K864400
Ptac, trp & lac regulated promoter
2012-09-24T11:00:00Z
2015-05-08T01:13:37Z
Created from oligos ordered from SigmaAldrich.
Oligo sequences:
Ptac+
AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA
Ptac-
CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
Released HQ 2013
The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]]
[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
false
false
_1124_
0
9827
9
In stock
false
Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.
false
Erik Lundin
annotation2197238
1
-10
range2197238
1
29
33
annotation2197240
1
lacO1 site
range2197240
1
36
61
annotation2197236
1
Ptac
range2197236
1
1
40
annotation2197237
1
-35
range2197237
1
7
12
BBa_K823017
1
BBa_K823017
double terminator (B0012-B0011)
2012-09-10T11:00:00Z
2015-05-08T01:13:30Z
Part:BBa_B0014
Released HQ 2013
This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
false
false
_1081_
0
11555
9
In stock
false
This is a copy of the [[Part:B0014|Part:B0014]]. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
false
Jara Radeck
component2182657
1
BBa_B0014
annotation2182657
1
BBa_B0014
range2182657
1
1
95
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K1813056_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgggcttagaagcattagttccattagcagtgatcgtggcaatttttttattgttggtggatttaatgcatcgccgtcagcgctgggcagcccgttatccacctggccctttgccattgcctggtttaggcaacttattacatgtggactttcaaaacactccttactgttttgatcaattacgtcgtcgctttggtgacgtattctcattacaattggcatggaccccagttgttgtgttgaatggcttggcagctgtgcgcgaggctttagttacccacggcgaggataccgccgaccgtcctccagttcctatcactcagatcttgggctttggccctcgtagtcaaggcgttttcttagcacgctacggcccagcttggcgtgaacagcgccgctttagtgtgtctaccttacgtaatttgggtttgggtaaaaaatcattggagcagtgggttaccgaggaagccgcttgcttgtgcgccgcctttgccaaccactcaggtcgtcctttccgccctaatggtttgttggataaagctgtgtcaaacgtgatcgcatcattgacatgcggtcgccgttttgagtacgatgatcctcgctttttacgtttgttggatttagcacaggaaggtttaaaggaagaatctggttttttgcgtgaggtgttaaacgccgtgcctgtgttgttacacattcctgcattagcaggtaaagtattacgcttccaaaaggctttcttgacacaattagatgaattattgactgaacaccgcatgacctgggacccagcacagccaccacgcgatttaaccgaagcttttttggccgaaatggaaaaagctaaaggcaacccagaatcatctttcaatgacgaaaacttacgtattgtagttgcagatttattttctgccggcatggtaaccacttcaaccactttggcctggggcttgttattgatgatcttgcaccctgacgtgcaacgtcgtgttcaacaggagatcgatgacgttatcggtcaggttcgtcgtccagagatgggtgatcaggcacatatgccttatacaactgctgtgatccatgaagtacagcgttttggcgatattgtgcctttaggcatgacccatatgaccagtcgcgatattgaggtgcaaggtttccgcattccaaagggcaccacattgattaccaacttatcatctgttttgaaagatgaagccgtgtgggaaaaaccattccgtttccatcctgagcattttttggatgcccaaggtcatttcgttaaaccagaagcatttttacctttttcagcaggtcgtcgtgcatgcttaggtgagcctttggcacgtatggagttattcttattctttaccagtttgttacagcattttagttttagtgtgcctaccggccagccacgtcctagtcaccatggtgtattcgcctttttggtttcaccaagtccatatgaattgtgcgcagttccacgctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K823017_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K1813037_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_K1813055_sequence
1
atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgggcttagaagcattagttccattagcagtgatcgtggcaatttttttattgttggtggatttaatgcatcgccgtcagcgctgggcagcccgttatccacctggccctttgccattgcctggtttaggcaacttattacatgtggactttcaaaacactccttactgttttgatcaattacgtcgtcgctttggtgacgtattctcattacaattggcatggaccccagttgttgtgttgaatggcttggcagctgtgcgcgaggctttagttacccacggcgaggataccgccgaccgtcctccagttcctatcactcagatcttgggctttggccctcgtagtcaaggcgttttcttagcacgctacggcccagcttggcgtgaacagcgccgctttagtgtgtctaccttacgtaatttgggtttgggtaaaaaatcattggagcagtgggttaccgaggaagccgcttgcttgtgcgccgcctttgccaaccactcaggtcgtcctttccgccctaatggtttgttggataaagctgtgtcaaacgtgatcgcatcattgacatgcggtcgccgttttgagtacgatgatcctcgctttttacgtttgttggatttagcacaggaaggtttaaaggaagaatctggttttttgcgtgaggtgttaaacgccgtgcctgtgttgttacacattcctgcattagcaggtaaagtattacgcttccaaaaggctttcttgacacaattagatgaattattgactgaacaccgcatgacctgggacccagcacagccaccacgcgatttaaccgaagcttttttggccgaaatggaaaaagctaaaggcaacccagaatcatctttcaatgacgaaaacttacgtattgtagttgcagatttattttctgccggcatggtaaccacttcaaccactttggcctggggcttgttattgatgatcttgcaccctgacgtgcaacgtcgtgttcaacaggagatcgatgacgttatcggtcaggttcgtcgtccagagatgggtgatcaggcacatatgccttatacaactgctgtgatccatgaagtacagcgttttggcgatattgtgcctttaggcatgacccatatgaccagtcgcgatattgaggtgcaaggtttccgcattccaaagggcaccacattgattaccaacttatcatctgttttgaaagatgaagccgtgtgggaaaaaccattccgtttccatcctgagcattttttggatgcccaaggtcatttcgttaaaccagaagcatttttacctttttcagcaggtcgtcgtgcatgcttaggtgagcctttggcacgtatggagttattcttattctttaccagtttgttacagcattttagttttagtgtgcctaccggccagccacgtcctagtcaccatggtgtattcgcctttttggtttcaccaagtccatatgaattgtgcgcagttccacgctaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K864400_sequence
1
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z