LOCUS BBa_K1813058 3149 bp DNA linear 26-Dec-2024 DEFINITION CYP6CM1 Expression Cassette with pelB ACCESSION BBa_K1813058 VERSION BBa_K1813058.1 FEATURES Location/Qualifiers promoter 1..1319 /label=BBa_K1813019 promoter 1328..1367 /label=Ptac promoter 1334..1339 /label=-35 promoter 1356..1360 /label=-10 misc_feature 1363..1388 /label=lacO1 site promoter 1328..1388 /label=BBa_K864400 misc_feature 1401..1404 /label=conserved RBS 1397..1408 /label=BBa_B0034 misc_feature 1328..1408 /label=BBa_K1813037 misc_feature 1415..1480 /label=pelB CDS 1415..3046 /label=BBa_K1813051 stem_loop 3062..3081 /label=T7 TE misc_feature 3088..3088 /label=stop polyA_site 3082..3095 /label=polya misc_feature 3055..3095 /label=BBa_B0012 terminator 3055..3095 /label=BBa_B0012 stem_loop 3116..3138 /label=stem_loop misc_feature 3104..3149 /label=BBa_B0011 terminator 3104..3149 /label=BBa_B0011 terminator 3055..3149 /label=BBa_B0014 terminator 3055..3149 /label=BBa_K823017 misc_feature 1328..3149 /label=BBa_K1813052 ORIGIN 1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa 61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac 121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga 301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg 421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg 481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg 541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc 601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc 661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat 721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg 781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc 841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc 901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac 961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac 1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc 1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt 1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa 1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag 1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat 1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga 1381 taacaattta ctagagaaag aggagaaata ctagatgaaa tacctgctgc cgaccgctgc 1441 tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc atggaattat tagaaatcgt 1501 aaagtcagcc atggatacac attctgtatt attattctta tcagtgatgg tatatttgtt 1561 atacgtgtat cgcgataagt ttcattactg gtcaaaacgt ggcgttcctt gccaaagtcc 1621 agcacagtca attgttcgta ccttccgttt agtattgcgc atggattctt ttaccgacaa 1681 cttctatcgt gtgtataagg cattcgacgg tcatccttat gtgggttctt tagagttaac 1741 caaaccaatc ttagtagtgc gcgatcctga attggcacgc attgtgttag ttaaatcttt 1801 tagtagtttt tctggccgtt taaaatctcc agacacaaca ttggacccat taagtaatca 1861 tttattcact ttaaacggtg aaaaatggcg ccaggtacgt cacaaaactg ccaccgcttt 1921 ttctaccgcc aagttaaaaa acatgtttca cagtttgaag gattgtgctc gtgaaatgga 1981 tgcctatatg gaacgcgcaa ttggtgataa aggcgatgtg gaatttgatg cttttaaagt 2041 gatgtctaat tatactttag aagttatcgg cgcttgtgct atgggcatca aatgcgattc 2101 tatccatgac gaagaaaccg aatttaaacg tttaagtcgc gatttctttc gttttgacgc 2161 tcgccgcatg atcttcacat tgttggattt attacaccct aaattgcctg ttttattgaa 2221 atggaaagca gtacgccctg aggtagaaaa cttcttccgc gaagccatta aagaagcagc 2281 ttctttgaaa gagtcagaag cagccgctcg caccgatttt ttacagattt taatcgattt 2341 tcagaaatct gaaaaagctt caaaaaccga tgctggtaac gacactgaat tggtgtttac 2401 cgataacatc attggtggcg ttatcggttc tttcttttca gctggctatg agcctaccgc 2461 tgctgctttg actttttgtt tgtatgaatt agctcgtcac cctcaggttc aggccaaatt 2521 acatgaaggc atcttagcag taaaggaaaa gttgggcgat gatatcgaat atgaaaactt 2581 aaaagagttt aaatatgcaa atcaagtaat cgatgaaact ttgcgtttgt accctgccag 2641 tggtattttg gtgcgtactt gtacagagcc ttttaaatta ccagattctg acgttgtgat 2701 tgaaaaaggc acaaaagtgt tcgtgtcatc atatggctta cagaccgatc ctcgctactt 2761 tcctgagcct gagaaatttg atccagaacg cttctcagaa gaaaataaag agaaaatttt 2821 gccaggtacc tacttaactt tcggtgatgg ccctcgctta tgcattgcaa tgcgtttagc 2881 tttgatggac gtgaaaatga tgatggttcg tttggtgtca aaatatgaaa ttcataccac 2941 cccaaagacc ccaaaaaaaa ttacctttga cactaactca tttactgtgc agccagccga 3001 aaaagtgtgg ttacgttttc gccgccgcgc aagtacccct taataatact agagtcacac 3061 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc 3121 agattattaa tccggctttt ttattattt //