LOCUS BBa_K1813061 4141 bp DNA linear 16-Dec-2024 DEFINITION ptac pelB AgCPR pelb CYP6G1 ACCESSION BBa_K1813061 VERSION BBa_K1813061.1 FEATURES Location/Qualifiers promoter 1..40 /label=Ptac promoter 7..12 /label=-35 promoter 29..33 /label=-10 misc_feature 36..61 /label=lacO1 site promoter 1..61 /label=BBa_K864400 misc_feature 74..77 /label=conserved RBS 70..81 /label=BBa_B0034 misc_feature 1..81 /label=BBa_K1813037 misc_feature 88..153 /label=pelB CDS 88..2196 /label=BBa_K1813053 stem_loop 2212..2231 /label=T7 TE misc_feature 2238..2238 /label=stop polyA_site 2232..2245 /label=polya misc_feature 2205..2245 /label=BBa_B0012 terminator 2205..2245 /label=BBa_B0012 stem_loop 2266..2288 /label=stem_loop misc_feature 2254..2299 /label=BBa_B0011 terminator 2254..2299 /label=BBa_B0011 terminator 2205..2299 /label=BBa_B0014 terminator 2205..2299 /label=BBa_K823017 misc_feature 1..2299 /label=BBa_K1813054 promoter 2308..2347 /label=Ptac promoter 2314..2319 /label=-35 promoter 2336..2340 /label=-10 misc_feature 2343..2368 /label=lacO1 site promoter 2308..2368 /label=BBa_K864400 misc_feature 2381..2384 /label=conserved RBS 2377..2388 /label=BBa_B0034 misc_feature 2308..2388 /label=BBa_K1813037 misc_feature 2395..2460 /label=pelB CDS 2395..4038 /label=BBa_K1813046 stem_loop 4054..4073 /label=T7 TE misc_feature 4080..4080 /label=stop polyA_site 4074..4087 /label=polya misc_feature 4047..4087 /label=BBa_B0012 terminator 4047..4087 /label=BBa_B0012 stem_loop 4108..4130 /label=stem_loop misc_feature 4096..4141 /label=BBa_B0011 terminator 4096..4141 /label=BBa_B0011 terminator 4047..4141 /label=BBa_B0014 terminator 4047..4141 /label=BBa_K823017 misc_feature 2308..4141 /label=BBa_K1813050 ORIGIN 1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat 61 ttactagaga aagaggagaa atactagatg aaatacctgc tgccgaccgc tgctgctggt 121 ctgctgctcc tcgctgccca gccggcgatg gccatggacg ctcagaccga aaccgaagtg 181 ccagcaggct ctgtgagtga tgaacctttc ttaggtcctt tagacatcgt tttattggta 241 tctttattgg ccggtacagc atggtatttg ttgaaaggca agaaaaaaga aagtcaggca 301 tcacagttta aatcatatag tattcaacca acaaccgtga atacaatgac catggtggaa 361 aacagtttca tcaagaaatt acagagttct ggtcgtcgct tagttgtttt ttatggttca 421 cagaccggta cagcagaaga atttgccggt cgcttagcta aagaaggcat ccgttatcag 481 atgaaaggta tggtagcaga tccagaagaa tgtaacatgg aagagttgtt aatgttaaaa 541 gacatcgaca agtcattggc tgtgttttgc ttagcaacct atggtgaagg tgatccaacc 601 gataactgta tggaatttta tgattggatc cagaacaatg atttagatat gactggcttg 661 aattacgctg tgtttggttt gggtaataaa acctacgagc attataataa agttggcatt 721 tacgttgata aacgcttgga agaattgggt gcaaaccgtg tttttgaatt gggcttgggc 781 gatgacgatg ccaacattga agattatttc atcacctgga aagaaaagtt ttggccaaca 841 gtttgcgatt atttcggtat tgaaagtaca ggcgaagacg ttttgatgcg tcagtatcgc 901 ttattggagc aaccagatgt gtcagccgat cgcatttaca ccggcgaggt ggcacgctta 961 cacagtttgc aaacccaacg cccacctttt gacgccaaaa accctttctt ggctccaatc 1021 aaagttaatc gtgagttgca taaagcaggt ggccgtagtt gcatgcatgt ggaatttgat 1081 atcgaaggta gtaaaatgcg ttatgaggct ggtgaccatt tagccatgta tcctgttaac 1141 gatcgcgatt tggttgagcg tttaggtcgt ttatgtaacg ctgaattgga caccgttttc 1201 tctttaatca acacagacac agattcttca aaaaaacatc cattcccttg cccaaccact 1261 taccgtactg ccttaaccca ctacttagaa atcacagcct tgccacgtac ccacattttg 1321 aaagaattag ctgaatattg cggtgaggaa aaagataaag aatttttgcg ttttatctct 1381 tctaccgcac cagatggcaa ggcaaagtat caggagtggg ttcaggatag ttgtcgcaac 1441 atcgtacacg tgttggaaga cattccttct tgccaccctc ctattgacca cgtgtgtgag 1501 ttattaccac gtttacaacc tcgctaccac tctatttctt ctagttcaaa attacaccct 1561 accacagttc atgtgaccgc agtattagta aaatatgaaa ccaaaaccgg tcgcttaaat 1621 aaaggtgtag ctaccacttt cttggccgag aaacatccaa atgatggcga accagcccca 1681 cgtgttccaa tttttatccg caaatctcag tttcgtttgc ctccaaaacc agaaacccca 1741 gtgattatgg tgggcccagg taccggtttg gctcctttcc gtggttttat tcaggagcgc 1801 gaccattgca aacaggaggg caaagaaatt ggtcagacca ctttatattt tggctgtcgc 1861 aaacgtagtg aagactatat ctatgaagac gaattggagg actacagtaa acgcggcatc 1921 atcaatttac gtgtggcctt ttcacgcgat caagagaaaa aagtgtatgt gactcactta 1981 ttagaacagg actctgattt gatttggagt gtgatcggtg aaaataaggg tcatttctat 2041 atttgcggtg acgccaaaaa catggctact gatgtgcgca acatcttgtt gaaagtaatt 2101 cgtagtaaag gcggcttatc agagaccgaa gctcaacaat acatcaagaa gatggaggca 2161 cagaagcgtt attctgctga tgtatggtca taataatact agagtcacac tggctcacct 2221 tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa 2281 tccggctttt ttattatttt actagaggag ctgttgacaa ttaatcatcg gctcgtataa 2341 tgtgtggaat tgtgagcgga taacaattta ctagagaaag aggagaaata ctagatgaaa 2401 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc 2461 atggtattaa cagaagtttt attcgtagtt gttgccgcct tagtagcatt atatacctgg 2521 ttccgccgta accattcata ctggcaacgc aaaggtatcc cttatattcc tccaactcct 2581 atcattggta atacaaaggt ggtgttcaag atggagaact ctttcggtat gcacttaagt 2641 gaaatttata acgatccacg tttgaaagat gaggctgtgg taggcatcta ttcaatgaac 2701 aaacctggct taatcatccg cgatatcgag ttgattaagt caatcttgat caaagatttt 2761 aaccgttttc ataatcgtta tgcccgttgc gatcctcacg gcgacccatt aggctataac 2821 aacttgttct ttgtacgtga tgcccattgg aaaggcattc gcactaaatt aacccctgtg 2881 tttaccagtg gtaaggtaaa gcagatgtat acattaatgc aggaaattgg caaagactta 2941 gaattagcat tgcaacgccg tggcgaaaaa aatacaggca gttttatcac cgagattaaa 3001 gaaatttgtg cccagttttc aacagatagt atcgcaacta tcgccttcgg tattcgcgcc 3061 aattctttgg aaaacccaaa tgctgaattt cgtaactacg gccgtaaaat gtttaccttc 3121 accgtagctc gtgcaaaaga tttcttcgta gccttcttct tacctaaatt ggtaagtttg 3181 atgcgtatcc agtttttcac agctgacttt tcacacttca tgcgctctac catcggtcac 3241 gttatggagg agcgcgaacg ctctggttta ttacgcaacg atttaatcga tgttttggtg 3301 agtttacgta aagaggccgc agccgaacca agtaaaccac attatgccaa gaaccaggat 3361 tttttagtgg ctcaggctgg tgtatttttc acagccggct ttgagacctc ttctagtacc 3421 atgtcttttg cattatacga gatggccaag catccagaaa tgcagaaacg cttgcgtgac 3481 gagattaacg aagctttagt ggaaggcggc ggttctttat catatgaaaa aatccagtca 3541 ttggaatatt tagctatggt ggttgatgaa gttttgcgta tgtacccagt tttgccattc 3601 ttggatcgcg agtacgagtc tgttgagggc cagcctgatt tgtctttaaa accattctat 3661 gattatacct tagaaaacgg cactccagta tttattccta tctacgcttt acatcatgat 3721 ccaaagtatt ggacaaaccc tagtcaattt gacccagagc gcttttctcc agccaaccgc 3781 aaaaacatcg ttgctatggc ctaccaacct tttggcagtg gtccacacaa ctgtatcggc 3841 agtcgcatcg gcttgttgca aagtaagtta ggtttggttt cattgttgaa aaaccatagt 3901 gtgcgtaact gtgaagcaac catgaaggat atgaaattcg atcctaaagg tttcgtttta 3961 caggccgatg gtggtattca tttggaaatc gtaaatgatc gcttatacga ccagagtgcc 4021 ccaagtttgc agtaataata ctagagtcac actggctcac cttcgggtgg gcctttctgc 4081 gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt 4141 t //