##gff-version 3 ##sequence-region BBa_K1813064 1 5468 BBa_K1813064 . engineered_region 1 3626 . + 0 ID=BBa_K1813059;Name=BBa_K1813059 BBa_K1813064 . engineered_region 1328 3626 . + 0 ID=BBa_K1813054;Name=BBa_K1813054;Parent=BBa_K1813059 BBa_K1813064 . engineered_region 1328 1408 . + 0 ID=BBa_K1813037;Name=BBa_K1813037;Parent=BBa_K1813054 BBa_K1813064 . ribosome_entry_site 1397 1408 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813037 BBa_K1813064 . conserved_region 1401 1404 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1813064 . promoter 1328 1388 . + 0 ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813037 BBa_K1813064 . promoter 1356 1360 . + 0 ID=annotation2197238;Name=-10;Parent=BBa_K864400 BBa_K1813064 . promoter 1334 1339 . + 0 ID=annotation2197237;Name=-35;Parent=BBa_K864400 BBa_K1813064 . promoter 1328 1367 . + 0 ID=annotation2197236;Name=Ptac;Parent=BBa_K864400 BBa_K1813064 . operator 1363 1388 . + 0 ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400 BBa_K1813064 . CDS 1415 3523 . + 0 ID=BBa_K1813053;Name=BBa_K1813053;Parent=BBa_K1813054 BBa_K1813064 . sequence_feature 1415 1480 . + 0 ID=annotation2449907;Name=pelB;Parent=BBa_K1813053 BBa_K1813064 . terminator 3532 3626 . + 0 ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813054 BBa_K1813064 . terminator 3532 3626 . + 0 ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017 BBa_K1813064 . terminator 3581 3626 . + 0 ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014 BBa_K1813064 . engineered_region 3581 3626 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_K1813064 . stem_loop 3593 3615 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_K1813064 . terminator 3532 3572 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014 BBa_K1813064 . polyA_site 3559 3572 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1813064 . stem_loop 3539 3558 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1813064 . engineered_region 3532 3572 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1813064 . stop_codon 3565 3565 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1813064 . promoter 1 1319 . + 0 ID=BBa_K1813019;Name=BBa_K1813019;Parent=BBa_K1813059 BBa_K1813064 . engineered_region 3635 5468 . + 0 ID=BBa_K1813050;Name=BBa_K1813050 BBa_K1813064 . terminator 5374 5468 . + 0 ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813050 BBa_K1813064 . terminator 5374 5468 . + 0 ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017 BBa_K1813064 . terminator 5423 5468 . + 0 ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014 BBa_K1813064 . engineered_region 5423 5468 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_K1813064 . stem_loop 5435 5457 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_K1813064 . terminator 5374 5414 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014 BBa_K1813064 . polyA_site 5401 5414 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1813064 . stem_loop 5381 5400 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1813064 . engineered_region 5374 5414 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1813064 . stop_codon 5407 5407 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1813064 . CDS 3722 5365 . + 0 ID=BBa_K1813046;Name=BBa_K1813046;Parent=BBa_K1813050 BBa_K1813064 . sequence_feature 3722 3787 . + 0 ID=annotation2449868;Name=pelB;Parent=BBa_K1813046 BBa_K1813064 . engineered_region 3635 3715 . + 0 ID=BBa_K1813037;Name=BBa_K1813037;Parent=BBa_K1813050 BBa_K1813064 . ribosome_entry_site 3704 3715 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813037 BBa_K1813064 . conserved_region 3708 3711 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1813064 . promoter 3635 3695 . + 0 ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813037 BBa_K1813064 . promoter 3663 3667 . + 0 ID=annotation2197238;Name=-10;Parent=BBa_K864400 BBa_K1813064 . promoter 3641 3646 . + 0 ID=annotation2197237;Name=-35;Parent=BBa_K864400 BBa_K1813064 . promoter 3635 3674 . + 0 ID=annotation2197236;Name=Ptac;Parent=BBa_K864400 BBa_K1813064 . operator 3670 3695 . + 0 ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400 >BBa_K1813064 aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccga aggtgagccagtgtgactctagtagagatctacactagcactatcagagttattaagctactaaagcgtagttttcgtc gtttgcagcctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag gcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcct ggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacgg cgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcg gtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagca tttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagt gagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctgg tgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtct ggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcgg atagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgt tctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgt gcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaat gtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgg gaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccatctagtatt tctcctctttctctagtaggcgctatcatgccataccgcgaaaggttttgcaccatactagaggagctgttgacaatta atcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggagaaatactagatgaaata cctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggacgctcagaccgaaacc gaagtgccagcaggctctgtgagtgatgaacctttcttaggtcctttagacatcgttttattggtatctttattggccg gtacagcatggtatttgttgaaaggcaagaaaaaagaaagtcaggcatcacagtttaaatcatatagtattcaaccaac aaccgtgaatacaatgaccatggtggaaaacagtttcatcaagaaattacagagttctggtcgtcgcttagttgttttt tatggttcacagaccggtacagcagaagaatttgccggtcgcttagctaaagaaggcatccgttatcagatgaaaggta tggtagcagatccagaagaatgtaacatggaagagttgttaatgttaaaagacatcgacaagtcattggctgtgttttg cttagcaacctatggtgaaggtgatccaaccgataactgtatggaattttatgattggatccagaacaatgatttagat atgactggcttgaattacgctgtgtttggtttgggtaataaaacctacgagcattataataaagttggcatttacgttg ataaacgcttggaagaattgggtgcaaaccgtgtttttgaattgggcttgggcgatgacgatgccaacattgaagatta tttcatcacctggaaagaaaagttttggccaacagtttgcgattatttcggtattgaaagtacaggcgaagacgttttg atgcgtcagtatcgcttattggagcaaccagatgtgtcagccgatcgcatttacaccggcgaggtggcacgcttacaca gtttgcaaacccaacgcccaccttttgacgccaaaaaccctttcttggctccaatcaaagttaatcgtgagttgcataa agcaggtggccgtagttgcatgcatgtggaatttgatatcgaaggtagtaaaatgcgttatgaggctggtgaccattta gccatgtatcctgttaacgatcgcgatttggttgagcgtttaggtcgtttatgtaacgctgaattggacaccgttttct ctttaatcaacacagacacagattcttcaaaaaaacatccattcccttgcccaaccacttaccgtactgccttaaccca ctacttagaaatcacagccttgccacgtacccacattttgaaagaattagctgaatattgcggtgaggaaaaagataaa gaatttttgcgttttatctcttctaccgcaccagatggcaaggcaaagtatcaggagtgggttcaggatagttgtcgca acatcgtacacgtgttggaagacattccttcttgccaccctcctattgaccacgtgtgtgagttattaccacgtttaca acctcgctaccactctatttcttctagttcaaaattacaccctaccacagttcatgtgaccgcagtattagtaaaatat gaaaccaaaaccggtcgcttaaataaaggtgtagctaccactttcttggccgagaaacatccaaatgatggcgaaccag ccccacgtgttccaatttttatccgcaaatctcagtttcgtttgcctccaaaaccagaaaccccagtgattatggtggg cccaggtaccggtttggctcctttccgtggttttattcaggagcgcgaccattgcaaacaggagggcaaagaaattggt cagaccactttatattttggctgtcgcaaacgtagtgaagactatatctatgaagacgaattggaggactacagtaaac gcggcatcatcaatttacgtgtggccttttcacgcgatcaagagaaaaaagtgtatgtgactcacttattagaacagga ctctgatttgatttggagtgtgatcggtgaaaataagggtcatttctatatttgcggtgacgccaaaaacatggctact gatgtgcgcaacatcttgttgaaagtaattcgtagtaaaggcggcttatcagagaccgaagctcaacaatacatcaaga agatggaggcacagaagcgttattctgctgatgtatggtcataataatactagagtcacactggctcaccttcgggtgg gcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagag gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggaga aatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggt attaacagaagttttattcgtagttgttgccgccttagtagcattatatacctggttccgccgtaaccattcatactgg caacgcaaaggtatcccttatattcctccaactcctatcattggtaatacaaaggtggtgttcaagatggagaactctt tcggtatgcacttaagtgaaatttataacgatccacgtttgaaagatgaggctgtggtaggcatctattcaatgaacaa acctggcttaatcatccgcgatatcgagttgattaagtcaatcttgatcaaagattttaaccgttttcataatcgttat gcccgttgcgatcctcacggcgacccattaggctataacaacttgttctttgtacgtgatgcccattggaaaggcattc gcactaaattaacccctgtgtttaccagtggtaaggtaaagcagatgtatacattaatgcaggaaattggcaaagactt agaattagcattgcaacgccgtggcgaaaaaaatacaggcagttttatcaccgagattaaagaaatttgtgcccagttt tcaacagatagtatcgcaactatcgccttcggtattcgcgccaattctttggaaaacccaaatgctgaatttcgtaact acggccgtaaaatgtttaccttcaccgtagctcgtgcaaaagatttcttcgtagccttcttcttacctaaattggtaag tttgatgcgtatccagtttttcacagctgacttttcacacttcatgcgctctaccatcggtcacgttatggaggagcgc gaacgctctggtttattacgcaacgatttaatcgatgttttggtgagtttacgtaaagaggccgcagccgaaccaagta aaccacattatgccaagaaccaggattttttagtggctcaggctggtgtatttttcacagccggctttgagacctcttc tagtaccatgtcttttgcattatacgagatggccaagcatccagaaatgcagaaacgcttgcgtgacgagattaacgaa gctttagtggaaggcggcggttctttatcatatgaaaaaatccagtcattggaatatttagctatggtggttgatgaag ttttgcgtatgtacccagttttgccattcttggatcgcgagtacgagtctgttgagggccagcctgatttgtctttaaa accattctatgattataccttagaaaacggcactccagtatttattcctatctacgctttacatcatgatccaaagtat tggacaaaccctagtcaatttgacccagagcgcttttctccagccaaccgcaaaaacatcgttgctatggcctaccaac cttttggcagtggtccacacaactgtatcggcagtcgcatcggcttgttgcaaagtaagttaggtttggtttcattgtt gaaaaaccatagtgtgcgtaactgtgaagcaaccatgaaggatatgaaattcgatcctaaaggtttcgttttacaggcc gatggtggtattcatttggaaatcgtaaatgatcgcttatacgaccagagtgccccaagtttgcagtaataatactaga gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatc cggcttttttattattt