LOCUS BBa_K1813065 5456 bp DNA linear 22-Nov-2024 DEFINITION LacIR pelB AgCPR ptac pelB CYP6CM1 ACCESSION BBa_K1813065 VERSION BBa_K1813065.1 FEATURES Location/Qualifiers promoter 1..1319 /label=BBa_K1813019 promoter 1328..1367 /label=Ptac promoter 1334..1339 /label=-35 promoter 1356..1360 /label=-10 misc_feature 1363..1388 /label=lacO1 site promoter 1328..1388 /label=BBa_K864400 misc_feature 1401..1404 /label=conserved RBS 1397..1408 /label=BBa_B0034 misc_feature 1328..1408 /label=BBa_K1813037 misc_feature 1415..1480 /label=pelB CDS 1415..3523 /label=BBa_K1813053 stem_loop 3539..3558 /label=T7 TE misc_feature 3565..3565 /label=stop polyA_site 3559..3572 /label=polya misc_feature 3532..3572 /label=BBa_B0012 terminator 3532..3572 /label=BBa_B0012 stem_loop 3593..3615 /label=stem_loop misc_feature 3581..3626 /label=BBa_B0011 terminator 3581..3626 /label=BBa_B0011 terminator 3532..3626 /label=BBa_B0014 terminator 3532..3626 /label=BBa_K823017 misc_feature 1328..3626 /label=BBa_K1813054 misc_feature 1..3626 /label=BBa_K1813059 promoter 3635..3674 /label=Ptac promoter 3641..3646 /label=-35 promoter 3663..3667 /label=-10 misc_feature 3670..3695 /label=lacO1 site promoter 3635..3695 /label=BBa_K864400 misc_feature 3708..3711 /label=conserved RBS 3704..3715 /label=BBa_B0034 misc_feature 3635..3715 /label=BBa_K1813037 misc_feature 3722..3787 /label=pelB CDS 3722..5353 /label=BBa_K1813051 stem_loop 5369..5388 /label=T7 TE misc_feature 5395..5395 /label=stop polyA_site 5389..5402 /label=polya misc_feature 5362..5402 /label=BBa_B0012 terminator 5362..5402 /label=BBa_B0012 stem_loop 5423..5445 /label=stem_loop misc_feature 5411..5456 /label=BBa_B0011 terminator 5411..5456 /label=BBa_B0011 terminator 5362..5456 /label=BBa_B0014 terminator 5362..5456 /label=BBa_K823017 misc_feature 3635..5456 /label=BBa_K1813052 ORIGIN 1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa 61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac 121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc 181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga 301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc 361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg 421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg 481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg 541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc 601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc 661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat 721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg 781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc 841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc 901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac 961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac 1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc 1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt 1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa 1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag 1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat 1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga 1381 taacaattta ctagagaaag aggagaaata ctagatgaaa tacctgctgc cgaccgctgc 1441 tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc atggacgctc agaccgaaac 1501 cgaagtgcca gcaggctctg tgagtgatga acctttctta ggtcctttag acatcgtttt 1561 attggtatct ttattggccg gtacagcatg gtatttgttg aaaggcaaga aaaaagaaag 1621 tcaggcatca cagtttaaat catatagtat tcaaccaaca accgtgaata caatgaccat 1681 ggtggaaaac agtttcatca agaaattaca gagttctggt cgtcgcttag ttgtttttta 1741 tggttcacag accggtacag cagaagaatt tgccggtcgc ttagctaaag aaggcatccg 1801 ttatcagatg aaaggtatgg tagcagatcc agaagaatgt aacatggaag agttgttaat 1861 gttaaaagac atcgacaagt cattggctgt gttttgctta gcaacctatg gtgaaggtga 1921 tccaaccgat aactgtatgg aattttatga ttggatccag aacaatgatt tagatatgac 1981 tggcttgaat tacgctgtgt ttggtttggg taataaaacc tacgagcatt ataataaagt 2041 tggcatttac gttgataaac gcttggaaga attgggtgca aaccgtgttt ttgaattggg 2101 cttgggcgat gacgatgcca acattgaaga ttatttcatc acctggaaag aaaagttttg 2161 gccaacagtt tgcgattatt tcggtattga aagtacaggc gaagacgttt tgatgcgtca 2221 gtatcgctta ttggagcaac cagatgtgtc agccgatcgc atttacaccg gcgaggtggc 2281 acgcttacac agtttgcaaa cccaacgccc accttttgac gccaaaaacc ctttcttggc 2341 tccaatcaaa gttaatcgtg agttgcataa agcaggtggc cgtagttgca tgcatgtgga 2401 atttgatatc gaaggtagta aaatgcgtta tgaggctggt gaccatttag ccatgtatcc 2461 tgttaacgat cgcgatttgg ttgagcgttt aggtcgttta tgtaacgctg aattggacac 2521 cgttttctct ttaatcaaca cagacacaga ttcttcaaaa aaacatccat tcccttgccc 2581 aaccacttac cgtactgcct taacccacta cttagaaatc acagccttgc cacgtaccca 2641 cattttgaaa gaattagctg aatattgcgg tgaggaaaaa gataaagaat ttttgcgttt 2701 tatctcttct accgcaccag atggcaaggc aaagtatcag gagtgggttc aggatagttg 2761 tcgcaacatc gtacacgtgt tggaagacat tccttcttgc caccctccta ttgaccacgt 2821 gtgtgagtta ttaccacgtt tacaacctcg ctaccactct atttcttcta gttcaaaatt 2881 acaccctacc acagttcatg tgaccgcagt attagtaaaa tatgaaacca aaaccggtcg 2941 cttaaataaa ggtgtagcta ccactttctt ggccgagaaa catccaaatg atggcgaacc 3001 agccccacgt gttccaattt ttatccgcaa atctcagttt cgtttgcctc caaaaccaga 3061 aaccccagtg attatggtgg gcccaggtac cggtttggct cctttccgtg gttttattca 3121 ggagcgcgac cattgcaaac aggagggcaa agaaattggt cagaccactt tatattttgg 3181 ctgtcgcaaa cgtagtgaag actatatcta tgaagacgaa ttggaggact acagtaaacg 3241 cggcatcatc aatttacgtg tggccttttc acgcgatcaa gagaaaaaag tgtatgtgac 3301 tcacttatta gaacaggact ctgatttgat ttggagtgtg atcggtgaaa ataagggtca 3361 tttctatatt tgcggtgacg ccaaaaacat ggctactgat gtgcgcaaca tcttgttgaa 3421 agtaattcgt agtaaaggcg gcttatcaga gaccgaagct caacaataca tcaagaagat 3481 ggaggcacag aagcgttatt ctgctgatgt atggtcataa taatactaga gtcacactgg 3541 ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga 3601 ttattaatcc ggctttttta ttattttact agaggagctg ttgacaatta atcatcggct 3661 cgtataatgt gtggaattgt gagcggataa caatttacta gagaaagagg agaaatacta 3721 gatgaaatac ctgctgccga ccgctgctgc tggtctgctg ctcctcgctg cccagccggc 3781 gatggccatg gaattattag aaatcgtaaa gtcagccatg gatacacatt ctgtattatt 3841 attcttatca gtgatggtat atttgttata cgtgtatcgc gataagtttc attactggtc 3901 aaaacgtggc gttccttgcc aaagtccagc acagtcaatt gttcgtacct tccgtttagt 3961 attgcgcatg gattctttta ccgacaactt ctatcgtgtg tataaggcat tcgacggtca 4021 tccttatgtg ggttctttag agttaaccaa accaatctta gtagtgcgcg atcctgaatt 4081 ggcacgcatt gtgttagtta aatcttttag tagtttttct ggccgtttaa aatctccaga 4141 cacaacattg gacccattaa gtaatcattt attcacttta aacggtgaaa aatggcgcca 4201 ggtacgtcac aaaactgcca ccgctttttc taccgccaag ttaaaaaaca tgtttcacag 4261 tttgaaggat tgtgctcgtg aaatggatgc ctatatggaa cgcgcaattg gtgataaagg 4321 cgatgtggaa tttgatgctt ttaaagtgat gtctaattat actttagaag ttatcggcgc 4381 ttgtgctatg ggcatcaaat gcgattctat ccatgacgaa gaaaccgaat ttaaacgttt 4441 aagtcgcgat ttctttcgtt ttgacgctcg ccgcatgatc ttcacattgt tggatttatt 4501 acaccctaaa ttgcctgttt tattgaaatg gaaagcagta cgccctgagg tagaaaactt 4561 cttccgcgaa gccattaaag aagcagcttc tttgaaagag tcagaagcag ccgctcgcac 4621 cgatttttta cagattttaa tcgattttca gaaatctgaa aaagcttcaa aaaccgatgc 4681 tggtaacgac actgaattgg tgtttaccga taacatcatt ggtggcgtta tcggttcttt 4741 cttttcagct ggctatgagc ctaccgctgc tgctttgact ttttgtttgt atgaattagc 4801 tcgtcaccct caggttcagg ccaaattaca tgaaggcatc ttagcagtaa aggaaaagtt 4861 gggcgatgat atcgaatatg aaaacttaaa agagtttaaa tatgcaaatc aagtaatcga 4921 tgaaactttg cgtttgtacc ctgccagtgg tattttggtg cgtacttgta cagagccttt 4981 taaattacca gattctgacg ttgtgattga aaaaggcaca aaagtgttcg tgtcatcata 5041 tggcttacag accgatcctc gctactttcc tgagcctgag aaatttgatc cagaacgctt 5101 ctcagaagaa aataaagaga aaattttgcc aggtacctac ttaactttcg gtgatggccc 5161 tcgcttatgc attgcaatgc gtttagcttt gatggacgtg aaaatgatga tggttcgttt 5221 ggtgtcaaaa tatgaaattc ataccacccc aaagacccca aaaaaaatta cctttgacac 5281 taactcattt actgtgcagc cagccgaaaa agtgtggtta cgttttcgcc gccgcgcaag 5341 taccccttaa taatactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 5401 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttattt //