LOCUS BBa_K1813073 5737 bp DNA linear 15-Nov-2024 DEFINITION ptac cch2 pelB AgCPR CYP6G1 ACCESSION BBa_K1813073 VERSION BBa_K1813073.1 FEATURES Location/Qualifiers promoter 1..40 /label=Ptac promoter 7..12 /label=-35 promoter 29..33 /label=-10 misc_feature 36..61 /label=lacO1 site promoter 1..61 /label=BBa_K864400 misc_feature 74..77 /label=conserved RBS 70..81 /label=BBa_B0034 CDS 88..1485 /label=BBa_K1813000 stem_loop 1501..1520 /label=T7 TE misc_feature 1527..1527 /label=stop polyA_site 1521..1534 /label=polya misc_feature 1494..1534 /label=BBa_B0012 terminator 1494..1534 /label=BBa_B0012 stem_loop 1555..1577 /label=stem_loop misc_feature 1543..1588 /label=BBa_B0011 terminator 1543..1588 /label=BBa_B0011 terminator 1494..1588 /label=BBa_B0014 terminator 1494..1588 /label=BBa_K823017 misc_feature 1..1588 /label=BBa_K1813001 promoter 1597..1636 /label=Ptac promoter 1603..1608 /label=-35 promoter 1625..1629 /label=-10 misc_feature 1632..1657 /label=lacO1 site promoter 1597..1657 /label=BBa_K864400 misc_feature 1670..1673 /label=conserved RBS 1666..1677 /label=BBa_B0034 misc_feature 1597..1677 /label=BBa_K1813037 misc_feature 1684..1749 /label=pelB CDS 1684..3792 /label=BBa_K1813053 stem_loop 3808..3827 /label=T7 TE misc_feature 3834..3834 /label=stop polyA_site 3828..3841 /label=polya misc_feature 3801..3841 /label=BBa_B0012 terminator 3801..3841 /label=BBa_B0012 stem_loop 3862..3884 /label=stem_loop misc_feature 3850..3895 /label=BBa_B0011 terminator 3850..3895 /label=BBa_B0011 terminator 3801..3895 /label=BBa_B0014 terminator 3801..3895 /label=BBa_K823017 misc_feature 1597..3895 /label=BBa_K1813054 promoter 3904..3943 /label=Ptac promoter 3910..3915 /label=-35 promoter 3932..3936 /label=-10 misc_feature 3939..3964 /label=lacO1 site promoter 3904..3964 /label=BBa_K864400 misc_feature 3977..3980 /label=conserved RBS 3973..3984 /label=BBa_B0034 misc_feature 3904..3984 /label=BBa_K1813037 misc_feature 3991..4056 /label=pelB CDS 3991..5634 /label=BBa_K1813046 stem_loop 5650..5669 /label=T7 TE misc_feature 5676..5676 /label=stop polyA_site 5670..5683 /label=polya misc_feature 5643..5683 /label=BBa_B0012 terminator 5643..5683 /label=BBa_B0012 stem_loop 5704..5726 /label=stem_loop misc_feature 5692..5737 /label=BBa_B0011 terminator 5692..5737 /label=BBa_B0011 terminator 5643..5737 /label=BBa_B0014 terminator 5643..5737 /label=BBa_K823017 misc_feature 3904..5737 /label=BBa_K1813050 misc_feature 1597..5737 /label=BBa_K1813061 ORIGIN 1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat 61 ttactagaga aagaggagaa atactagatg tcattgattg caattaagaa cgctgactgg 121 atcttaacca tggatggcgc ccgtcgcgta atcgcagacg gctctatttt aatcgataaa 181 gaccgtattc gcgccatcgg taaaagtgct gtgatcgaga ttcctccaga gacaaccaaa 241 gtgattgacg gtcgcggcaa agtggttttg ccaggcttaa ttgacagtca tacccaccac 301 tcattacact taggccgtgg cttggctgac gaatgtgaca ttcagacatt cttatatcgt 361 cgcttgtacc caatcgaagc cagtttaaat gacgaggatg cctatatctc agcattatta 421 tgccagttag aaatgattaa aagtggtacc acctctatca tcgatgctgg caactacttt 481 ccagaggcta ccttacgtgc atttggcaca acaggtatgc gtggcgttgt tgcacgttca 541 acatttgaca ttcctacttc atctttgggt tctttacctg ctcaggtgtt tgcagaggag 601 acaaatgtag ccttaaaacg tgccgaagaa tttgtagagc gtaactctgg cgcatgcgac 661 ggccgtgtgc aggcatggtt acagttgcgc gttttaccta attgtagtga tgagttgtgc 721 cgtggtttga aatctattgc tgaccgtttg ggcgttcgct atgaagcaca cgccgccttt 781 accaaagaag tgtatgaagc tagtaaatta cagttcggca agagtgaagt gcgtcgtttg 841 gatgatttag gtatcttagg cgaaggttta ttattagctc atatgggttg gttaacccca 901 cgcgatattt tgttattgat tagttcaaaa actaatgttg tattatgccc aacctctagt 961 gtacaccagg ctatgggttc tatcgccttc ggccacgtgc cagaattatt agaaatgggt 1021 gtgaacgttg cattaggtac tgatggtggt cctcacggca caaatgactt ggtacgccag 1081 attttcgtgg ccgcaggcgg ctataaagaa gttcgcttgg atgctacaat catgcctcca 1141 gaaacagttt tagaaatggc aaccgttaat ggtgcacgcg ccatgggtat gtctgaccag 1201 gtaggctcaa tcgagccagg taaaaaagcc gacattacaa tcttcgactc tcgccgccct 1261 gaatggcgcc cattacataa tcctgttgca aatttagtat attgcgcaaa cggtaattca 1321 gcagataccg tgattgtaga cggccgcatt ttaatggaaa accgtaaaat cttaaccttt 1381 aacgaggatg acgtgattac cgaagcacag cgtcgctcag ttgaaatcgg tgctcgtgct 1441 ggcttgttgg aatacggtcg cccacgctgg ccagttcatt aataatacta gagtcacact 1501 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca 1561 gattattaat ccggcttttt tattatttta ctagaggagc tgttgacaat taatcatcgg 1621 ctcgtataat gtgtggaatt gtgagcggat aacaatttac tagagaaaga ggagaaatac 1681 tagatgaaat acctgctgcc gaccgctgct gctggtctgc tgctcctcgc tgcccagccg 1741 gcgatggcca tggacgctca gaccgaaacc gaagtgccag caggctctgt gagtgatgaa 1801 cctttcttag gtcctttaga catcgtttta ttggtatctt tattggccgg tacagcatgg 1861 tatttgttga aaggcaagaa aaaagaaagt caggcatcac agtttaaatc atatagtatt 1921 caaccaacaa ccgtgaatac aatgaccatg gtggaaaaca gtttcatcaa gaaattacag 1981 agttctggtc gtcgcttagt tgttttttat ggttcacaga ccggtacagc agaagaattt 2041 gccggtcgct tagctaaaga aggcatccgt tatcagatga aaggtatggt agcagatcca 2101 gaagaatgta acatggaaga gttgttaatg ttaaaagaca tcgacaagtc attggctgtg 2161 ttttgcttag caacctatgg tgaaggtgat ccaaccgata actgtatgga attttatgat 2221 tggatccaga acaatgattt agatatgact ggcttgaatt acgctgtgtt tggtttgggt 2281 aataaaacct acgagcatta taataaagtt ggcatttacg ttgataaacg cttggaagaa 2341 ttgggtgcaa accgtgtttt tgaattgggc ttgggcgatg acgatgccaa cattgaagat 2401 tatttcatca cctggaaaga aaagttttgg ccaacagttt gcgattattt cggtattgaa 2461 agtacaggcg aagacgtttt gatgcgtcag tatcgcttat tggagcaacc agatgtgtca 2521 gccgatcgca tttacaccgg cgaggtggca cgcttacaca gtttgcaaac ccaacgccca 2581 ccttttgacg ccaaaaaccc tttcttggct ccaatcaaag ttaatcgtga gttgcataaa 2641 gcaggtggcc gtagttgcat gcatgtggaa tttgatatcg aaggtagtaa aatgcgttat 2701 gaggctggtg accatttagc catgtatcct gttaacgatc gcgatttggt tgagcgttta 2761 ggtcgtttat gtaacgctga attggacacc gttttctctt taatcaacac agacacagat 2821 tcttcaaaaa aacatccatt cccttgccca accacttacc gtactgcctt aacccactac 2881 ttagaaatca cagccttgcc acgtacccac attttgaaag aattagctga atattgcggt 2941 gaggaaaaag ataaagaatt tttgcgtttt atctcttcta ccgcaccaga tggcaaggca 3001 aagtatcagg agtgggttca ggatagttgt cgcaacatcg tacacgtgtt ggaagacatt 3061 ccttcttgcc accctcctat tgaccacgtg tgtgagttat taccacgttt acaacctcgc 3121 taccactcta tttcttctag ttcaaaatta caccctacca cagttcatgt gaccgcagta 3181 ttagtaaaat atgaaaccaa aaccggtcgc ttaaataaag gtgtagctac cactttcttg 3241 gccgagaaac atccaaatga tggcgaacca gccccacgtg ttccaatttt tatccgcaaa 3301 tctcagtttc gtttgcctcc aaaaccagaa accccagtga ttatggtggg cccaggtacc 3361 ggtttggctc ctttccgtgg ttttattcag gagcgcgacc attgcaaaca ggagggcaaa 3421 gaaattggtc agaccacttt atattttggc tgtcgcaaac gtagtgaaga ctatatctat 3481 gaagacgaat tggaggacta cagtaaacgc ggcatcatca atttacgtgt ggccttttca 3541 cgcgatcaag agaaaaaagt gtatgtgact cacttattag aacaggactc tgatttgatt 3601 tggagtgtga tcggtgaaaa taagggtcat ttctatattt gcggtgacgc caaaaacatg 3661 gctactgatg tgcgcaacat cttgttgaaa gtaattcgta gtaaaggcgg cttatcagag 3721 accgaagctc aacaatacat caagaagatg gaggcacaga agcgttattc tgctgatgta 3781 tggtcataat aatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 3841 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tattttacta 3901 gaggagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac 3961 aatttactag agaaagagga gaaatactag atgaaatacc tgctgccgac cgctgctgct 4021 ggtctgctgc tcctcgctgc ccagccggcg atggccatgg tattaacaga agttttattc 4081 gtagttgttg ccgccttagt agcattatat acctggttcc gccgtaacca ttcatactgg 4141 caacgcaaag gtatccctta tattcctcca actcctatca ttggtaatac aaaggtggtg 4201 ttcaagatgg agaactcttt cggtatgcac ttaagtgaaa tttataacga tccacgtttg 4261 aaagatgagg ctgtggtagg catctattca atgaacaaac ctggcttaat catccgcgat 4321 atcgagttga ttaagtcaat cttgatcaaa gattttaacc gttttcataa tcgttatgcc 4381 cgttgcgatc ctcacggcga cccattaggc tataacaact tgttctttgt acgtgatgcc 4441 cattggaaag gcattcgcac taaattaacc cctgtgttta ccagtggtaa ggtaaagcag 4501 atgtatacat taatgcagga aattggcaaa gacttagaat tagcattgca acgccgtggc 4561 gaaaaaaata caggcagttt tatcaccgag attaaagaaa tttgtgccca gttttcaaca 4621 gatagtatcg caactatcgc cttcggtatt cgcgccaatt ctttggaaaa cccaaatgct 4681 gaatttcgta actacggccg taaaatgttt accttcaccg tagctcgtgc aaaagatttc 4741 ttcgtagcct tcttcttacc taaattggta agtttgatgc gtatccagtt tttcacagct 4801 gacttttcac acttcatgcg ctctaccatc ggtcacgtta tggaggagcg cgaacgctct 4861 ggtttattac gcaacgattt aatcgatgtt ttggtgagtt tacgtaaaga ggccgcagcc 4921 gaaccaagta aaccacatta tgccaagaac caggattttt tagtggctca ggctggtgta 4981 tttttcacag ccggctttga gacctcttct agtaccatgt cttttgcatt atacgagatg 5041 gccaagcatc cagaaatgca gaaacgcttg cgtgacgaga ttaacgaagc tttagtggaa 5101 ggcggcggtt ctttatcata tgaaaaaatc cagtcattgg aatatttagc tatggtggtt 5161 gatgaagttt tgcgtatgta cccagttttg ccattcttgg atcgcgagta cgagtctgtt 5221 gagggccagc ctgatttgtc tttaaaacca ttctatgatt ataccttaga aaacggcact 5281 ccagtattta ttcctatcta cgctttacat catgatccaa agtattggac aaaccctagt 5341 caatttgacc cagagcgctt ttctccagcc aaccgcaaaa acatcgttgc tatggcctac 5401 caaccttttg gcagtggtcc acacaactgt atcggcagtc gcatcggctt gttgcaaagt 5461 aagttaggtt tggtttcatt gttgaaaaac catagtgtgc gtaactgtga agcaaccatg 5521 aaggatatga aattcgatcc taaaggtttc gttttacagg ccgatggtgg tattcatttg 5581 gaaatcgtaa atgatcgctt atacgaccag agtgccccaa gtttgcagta ataatactag 5641 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat 5701 aaaaagccag attattaatc cggctttttt attattt //