##gff-version 3
##sequence-region BBa_K1813074 1 5725
BBa_K1813074	.	engineered_region	1	1588	.	+	0	ID=BBa_K1813001;Name=BBa_K1813001
BBa_K1813074	.	CDS	88	1485	.	+	0	ID=BBa_K1813000;Name=BBa_K1813000;Parent=BBa_K1813001
BBa_K1813074	.	ribosome_entry_site	70	81	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813001
BBa_K1813074	.	conserved_region	74	77	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1813074	.	promoter	1	61	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813001
BBa_K1813074	.	promoter	29	33	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813074	.	promoter	1	40	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813074	.	operator	36	61	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813074	.	promoter	7	12	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
BBa_K1813074	.	terminator	1494	1588	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813001
BBa_K1813074	.	terminator	1494	1588	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813074	.	terminator	1543	1588	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813074	.	stem_loop	1555	1577	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813074	.	engineered_region	1543	1588	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813074	.	terminator	1494	1534	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813074	.	engineered_region	1494	1534	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813074	.	stop_codon	1527	1527	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813074	.	polyA_site	1521	1534	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813074	.	stem_loop	1501	1520	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813074	.	engineered_region	1597	5725	.	+	0	ID=BBa_K1813062;Name=BBa_K1813062
BBa_K1813074	.	engineered_region	1597	3895	.	+	0	ID=BBa_K1813054;Name=BBa_K1813054;Parent=BBa_K1813062
BBa_K1813074	.	engineered_region	1597	1677	.	+	0	ID=BBa_K1813037;Name=BBa_K1813037;Parent=BBa_K1813054
BBa_K1813074	.	ribosome_entry_site	1666	1677	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813037
BBa_K1813074	.	conserved_region	1670	1673	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1813074	.	promoter	1597	1657	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813037
BBa_K1813074	.	promoter	1625	1629	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813074	.	promoter	1597	1636	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813074	.	operator	1632	1657	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813074	.	promoter	1603	1608	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
BBa_K1813074	.	terminator	3801	3895	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813054
BBa_K1813074	.	terminator	3801	3895	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813074	.	terminator	3850	3895	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813074	.	stem_loop	3862	3884	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813074	.	engineered_region	3850	3895	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813074	.	terminator	3801	3841	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813074	.	engineered_region	3801	3841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813074	.	stop_codon	3834	3834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813074	.	polyA_site	3828	3841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813074	.	stem_loop	3808	3827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813074	.	CDS	1684	3792	.	+	0	ID=BBa_K1813053;Name=BBa_K1813053;Parent=BBa_K1813054
BBa_K1813074	.	sequence_feature	1684	1749	.	+	0	ID=annotation2449907;Name=pelB;Parent=BBa_K1813053
BBa_K1813074	.	engineered_region	3904	5725	.	+	0	ID=BBa_K1813052;Name=BBa_K1813052;Parent=BBa_K1813062
BBa_K1813074	.	terminator	5631	5725	.	+	0	ID=BBa_K823017;Name=BBa_K823017;Parent=BBa_K1813052
BBa_K1813074	.	terminator	5631	5725	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1813074	.	terminator	5680	5725	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1813074	.	stem_loop	5692	5714	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1813074	.	engineered_region	5680	5725	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1813074	.	terminator	5631	5671	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1813074	.	engineered_region	5631	5671	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1813074	.	stop_codon	5664	5664	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1813074	.	polyA_site	5658	5671	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1813074	.	stem_loop	5638	5657	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1813074	.	CDS	3991	5622	.	+	0	ID=BBa_K1813051;Name=BBa_K1813051;Parent=BBa_K1813052
BBa_K1813074	.	sequence_feature	3991	4056	.	+	0	ID=annotation2449887;Name=pelB;Parent=BBa_K1813051
BBa_K1813074	.	engineered_region	3904	3984	.	+	0	ID=BBa_K1813037;Name=BBa_K1813037;Parent=BBa_K1813052
BBa_K1813074	.	ribosome_entry_site	3973	3984	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1813037
BBa_K1813074	.	conserved_region	3977	3980	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1813074	.	promoter	3904	3964	.	+	0	ID=BBa_K864400;Name=BBa_K864400;Parent=BBa_K1813037
BBa_K1813074	.	promoter	3932	3936	.	+	0	ID=annotation2197238;Name=-10;Parent=BBa_K864400
BBa_K1813074	.	promoter	3904	3943	.	+	0	ID=annotation2197236;Name=Ptac;Parent=BBa_K864400
BBa_K1813074	.	operator	3939	3964	.	+	0	ID=annotation2197240;Name=lacO1 site;Parent=BBa_K864400
BBa_K1813074	.	promoter	3910	3915	.	+	0	ID=annotation2197237;Name=-35;Parent=BBa_K864400
>BBa_K1813074
gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagaaagaggaga
aatactagatgtcattgattgcaattaagaacgctgactggatcttaaccatggatggcgcccgtcgcgtaatcgcaga
cggctctattttaatcgataaagaccgtattcgcgccatcggtaaaagtgctgtgatcgagattcctccagagacaacc
aaagtgattgacggtcgcggcaaagtggttttgccaggcttaattgacagtcatacccaccactcattacacttaggcc
gtggcttggctgacgaatgtgacattcagacattcttatatcgtcgcttgtacccaatcgaagccagtttaaatgacga
ggatgcctatatctcagcattattatgccagttagaaatgattaaaagtggtaccacctctatcatcgatgctggcaac
tactttccagaggctaccttacgtgcatttggcacaacaggtatgcgtggcgttgttgcacgttcaacatttgacattc
ctacttcatctttgggttctttacctgctcaggtgtttgcagaggagacaaatgtagccttaaaacgtgccgaagaatt
tgtagagcgtaactctggcgcatgcgacggccgtgtgcaggcatggttacagttgcgcgttttacctaattgtagtgat
gagttgtgccgtggtttgaaatctattgctgaccgtttgggcgttcgctatgaagcacacgccgcctttaccaaagaag
tgtatgaagctagtaaattacagttcggcaagagtgaagtgcgtcgtttggatgatttaggtatcttaggcgaaggttt
attattagctcatatgggttggttaaccccacgcgatattttgttattgattagttcaaaaactaatgttgtattatgc
ccaacctctagtgtacaccaggctatgggttctatcgccttcggccacgtgccagaattattagaaatgggtgtgaacg
ttgcattaggtactgatggtggtcctcacggcacaaatgacttggtacgccagattttcgtggccgcaggcggctataa
agaagttcgcttggatgctacaatcatgcctccagaaacagttttagaaatggcaaccgttaatggtgcacgcgccatg
ggtatgtctgaccaggtaggctcaatcgagccaggtaaaaaagccgacattacaatcttcgactctcgccgccctgaat
ggcgcccattacataatcctgttgcaaatttagtatattgcgcaaacggtaattcagcagataccgtgattgtagacgg
ccgcattttaatggaaaaccgtaaaatcttaacctttaacgaggatgacgtgattaccgaagcacagcgtcgctcagtt
gaaatcggtgctcgtgctggcttgttggaatacggtcgcccacgctggccagttcattaataatactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttt
tattattttactagaggagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaattta
ctagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagc
cggcgatggccatggacgctcagaccgaaaccgaagtgccagcaggctctgtgagtgatgaacctttcttaggtccttt
agacatcgttttattggtatctttattggccggtacagcatggtatttgttgaaaggcaagaaaaaagaaagtcaggca
tcacagtttaaatcatatagtattcaaccaacaaccgtgaatacaatgaccatggtggaaaacagtttcatcaagaaat
tacagagttctggtcgtcgcttagttgttttttatggttcacagaccggtacagcagaagaatttgccggtcgcttagc
taaagaaggcatccgttatcagatgaaaggtatggtagcagatccagaagaatgtaacatggaagagttgttaatgtta
aaagacatcgacaagtcattggctgtgttttgcttagcaacctatggtgaaggtgatccaaccgataactgtatggaat
tttatgattggatccagaacaatgatttagatatgactggcttgaattacgctgtgtttggtttgggtaataaaaccta
cgagcattataataaagttggcatttacgttgataaacgcttggaagaattgggtgcaaaccgtgtttttgaattgggc
ttgggcgatgacgatgccaacattgaagattatttcatcacctggaaagaaaagttttggccaacagtttgcgattatt
tcggtattgaaagtacaggcgaagacgttttgatgcgtcagtatcgcttattggagcaaccagatgtgtcagccgatcg
catttacaccggcgaggtggcacgcttacacagtttgcaaacccaacgcccaccttttgacgccaaaaaccctttcttg
gctccaatcaaagttaatcgtgagttgcataaagcaggtggccgtagttgcatgcatgtggaatttgatatcgaaggta
gtaaaatgcgttatgaggctggtgaccatttagccatgtatcctgttaacgatcgcgatttggttgagcgtttaggtcg
tttatgtaacgctgaattggacaccgttttctctttaatcaacacagacacagattcttcaaaaaaacatccattccct
tgcccaaccacttaccgtactgccttaacccactacttagaaatcacagccttgccacgtacccacattttgaaagaat
tagctgaatattgcggtgaggaaaaagataaagaatttttgcgttttatctcttctaccgcaccagatggcaaggcaaa
gtatcaggagtgggttcaggatagttgtcgcaacatcgtacacgtgttggaagacattccttcttgccaccctcctatt
gaccacgtgtgtgagttattaccacgtttacaacctcgctaccactctatttcttctagttcaaaattacaccctacca
cagttcatgtgaccgcagtattagtaaaatatgaaaccaaaaccggtcgcttaaataaaggtgtagctaccactttctt
ggccgagaaacatccaaatgatggcgaaccagccccacgtgttccaatttttatccgcaaatctcagtttcgtttgcct
ccaaaaccagaaaccccagtgattatggtgggcccaggtaccggtttggctcctttccgtggttttattcaggagcgcg
accattgcaaacaggagggcaaagaaattggtcagaccactttatattttggctgtcgcaaacgtagtgaagactatat
ctatgaagacgaattggaggactacagtaaacgcggcatcatcaatttacgtgtggccttttcacgcgatcaagagaaa
aaagtgtatgtgactcacttattagaacaggactctgatttgatttggagtgtgatcggtgaaaataagggtcatttct
atatttgcggtgacgccaaaaacatggctactgatgtgcgcaacatcttgttgaaagtaattcgtagtaaaggcggctt
atcagagaccgaagctcaacaatacatcaagaagatggaggcacagaagcgttattctgctgatgtatggtcataataa
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagatt
attaatccggcttttttattattttactagaggagctgttgacaattaatcatcggctcgtataatgtgtggaattgtg
agcggataacaatttactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctg
ctcctcgctgcccagccggcgatggccatggaattattagaaatcgtaaagtcagccatggatacacattctgtattat
tattcttatcagtgatggtatatttgttatacgtgtatcgcgataagtttcattactggtcaaaacgtggcgttccttg
ccaaagtccagcacagtcaattgttcgtaccttccgtttagtattgcgcatggattcttttaccgacaacttctatcgt
gtgtataaggcattcgacggtcatccttatgtgggttctttagagttaaccaaaccaatcttagtagtgcgcgatcctg
aattggcacgcattgtgttagttaaatcttttagtagtttttctggccgtttaaaatctccagacacaacattggaccc
attaagtaatcatttattcactttaaacggtgaaaaatggcgccaggtacgtcacaaaactgccaccgctttttctacc
gccaagttaaaaaacatgtttcacagtttgaaggattgtgctcgtgaaatggatgcctatatggaacgcgcaattggtg
ataaaggcgatgtggaatttgatgcttttaaagtgatgtctaattatactttagaagttatcggcgcttgtgctatggg
catcaaatgcgattctatccatgacgaagaaaccgaatttaaacgtttaagtcgcgatttctttcgttttgacgctcgc
cgcatgatcttcacattgttggatttattacaccctaaattgcctgttttattgaaatggaaagcagtacgccctgagg
tagaaaacttcttccgcgaagccattaaagaagcagcttctttgaaagagtcagaagcagccgctcgcaccgatttttt
acagattttaatcgattttcagaaatctgaaaaagcttcaaaaaccgatgctggtaacgacactgaattggtgtttacc
gataacatcattggtggcgttatcggttctttcttttcagctggctatgagcctaccgctgctgctttgactttttgtt
tgtatgaattagctcgtcaccctcaggttcaggccaaattacatgaaggcatcttagcagtaaaggaaaagttgggcga
tgatatcgaatatgaaaacttaaaagagtttaaatatgcaaatcaagtaatcgatgaaactttgcgtttgtaccctgcc
agtggtattttggtgcgtacttgtacagagccttttaaattaccagattctgacgttgtgattgaaaaaggcacaaaag
tgttcgtgtcatcatatggcttacagaccgatcctcgctactttcctgagcctgagaaatttgatccagaacgcttctc
agaagaaaataaagagaaaattttgccaggtacctacttaactttcggtgatggccctcgcttatgcattgcaatgcgt
ttagctttgatggacgtgaaaatgatgatggttcgtttggtgtcaaaatatgaaattcataccaccccaaagaccccaa
aaaaaattacctttgacactaactcatttactgtgcagccagccgaaaaagtgtggttacgttttcgccgccgcgcaag
taccccttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatat
aaaaagccagattattaatccggcttttttattattt