BBa_K1819001 1 BBa_K1819001 Latex Clearing Protein (Lcp) - rubber degradation 2015-09-12T11:00:00Z 2015-09-13T08:46:53Z Lcp from actinomycete Streptomyces sp. strain K30 Latex clearing protein (Lcp) from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1) is a 42kDa enzyme which catalyzes rubber cleavage at the cis double bonds to multiple products ranging from C20 tetra-isoprenoid to at least C35 hepta-isoprenoid with measured specific activity of 1.3 mol/min per mg. false false _2244_ 21190 21190 9 false Because Lcp is secreted by Streptomyces sp, it contains a 5???-signal peptide that was identified using signal peptide predictor server (4) (http://www.compgen.org/tools/PRED-TAT). We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites. false Lais Ribovski annotation2453669 1 SacI site range2453669 1 1 6 annotation2453650 1 Lcp range2453650 1 7 1132 annotation2453670 1 NdeI site range2453670 1 1133 1138 BBa_K1819001_sequence 1 gagctcactgtggacgtggtcaccgagcgcgtcggtggcgggcacgggcgtaggcgtcgatccagaatacgtctgggacgaggaagccgacccggtattagccgcagtcattgaccgcggggaggtgcccgcggtcaatgcgctactgaaacagtggacgcggaacgaccaagcgctgcccggcggcctcccgggagacttgcgggagtttatggaacatgcacgccgtatgccttcgtgggcggacaaagcagcactcgaccgcggtgcccaattcagcaagaccaagggcatttacgtcggagccctgtacggcctcggcagtggactgatgagcactgccatacccagggagtcgcgcgccgtgtattactccaagggcggtgcggacatgaaggaccgaatcgctaagaccgcacgtctcggttacgacattggagacctggacgcgtatctgcctcatggctcaatgatcgtgaccgcagtgaagacacgcatggtgcacgcggctgttcgccacctgctgccgcagtcgccggcctggtcgcagaccagcggtggccagaagatcccgatcagccaggccgatatcatggtcacctggcacagcctggccactttcgtcatgcgcaagatgaagcagtggggcgtacgggtcaacaccgccgacgcggaggcttaccttcacgtgtggcaggtcagtgcgcacatgctcggagtcagcgacgagtacatccccgcgacctgggacgcggccaacgcgcagtcgaagcaggtcctcgacccgatcctcgcccacacgccggagggggaggcgctgacagaggtcctcctcggaatcgttgccgaactcgacgccggcctcacccgccccctgatcggcgcgttctcgaggtacacactcggcggcgaagtcggcgacatgatcggcctggccaagcagccggtcctggaacggctgatcgcgacggcctggcccctactcgtggccttccgtgaaggtctgataccacttcccgcggtcccggcggtgctctggacactcgaggaagcgctccgtaagttcgtcctgttgttcctctccgagggtcggcgcatcgccatcgacatcccggatgttaaccgcccgtcccatatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z