BBa_K1819001
1
BBa_K1819001
Latex Clearing Protein (Lcp) - rubber degradation
2015-09-12T11:00:00Z
2015-09-13T08:46:53Z
Lcp from actinomycete Streptomyces sp. strain K30
Latex clearing protein (Lcp) from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1) is a 42kDa enzyme which catalyzes rubber cleavage at the cis double bonds to multiple products ranging from C20 tetra-isoprenoid to at least C35 hepta-isoprenoid with measured specific activity of 1.3 mol/min per mg.
false
false
_2244_
21190
21190
9
false
Because Lcp is secreted by Streptomyces sp, it contains a 5???-signal peptide that was identified using signal peptide predictor server (4) (http://www.compgen.org/tools/PRED-TAT). We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites.
false
Lais Ribovski
annotation2453669
1
SacI site
range2453669
1
1
6
annotation2453650
1
Lcp
range2453650
1
7
1132
annotation2453670
1
NdeI site
range2453670
1
1133
1138
BBa_K1819001_sequence
1
gagctcactgtggacgtggtcaccgagcgcgtcggtggcgggcacgggcgtaggcgtcgatccagaatacgtctgggacgaggaagccgacccggtattagccgcagtcattgaccgcggggaggtgcccgcggtcaatgcgctactgaaacagtggacgcggaacgaccaagcgctgcccggcggcctcccgggagacttgcgggagtttatggaacatgcacgccgtatgccttcgtgggcggacaaagcagcactcgaccgcggtgcccaattcagcaagaccaagggcatttacgtcggagccctgtacggcctcggcagtggactgatgagcactgccatacccagggagtcgcgcgccgtgtattactccaagggcggtgcggacatgaaggaccgaatcgctaagaccgcacgtctcggttacgacattggagacctggacgcgtatctgcctcatggctcaatgatcgtgaccgcagtgaagacacgcatggtgcacgcggctgttcgccacctgctgccgcagtcgccggcctggtcgcagaccagcggtggccagaagatcccgatcagccaggccgatatcatggtcacctggcacagcctggccactttcgtcatgcgcaagatgaagcagtggggcgtacgggtcaacaccgccgacgcggaggcttaccttcacgtgtggcaggtcagtgcgcacatgctcggagtcagcgacgagtacatccccgcgacctgggacgcggccaacgcgcagtcgaagcaggtcctcgacccgatcctcgcccacacgccggagggggaggcgctgacagaggtcctcctcggaatcgttgccgaactcgacgccggcctcacccgccccctgatcggcgcgttctcgaggtacacactcggcggcgaagtcggcgacatgatcggcctggccaagcagccggtcctggaacggctgatcgcgacggcctggcccctactcgtggccttccgtgaaggtctgataccacttcccgcggtcccggcggtgctctggacactcgaggaagcgctccgtaagttcgtcctgttgttcctctccgagggtcggcgcatcgccatcgacatcccggatgttaaccgcccgtcccatatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z