BBa_K1819003 1 BBa_K1819003 Rubber oxygenase A - rubber degrading enzyme 2015-09-15T11:00:00Z 2015-09-16T07:53:47Z Xanthomonas sp. strain 35Y Part BBa_K1819003 corresponds to the coding sequence of rubber oxygenase A (Rox A) from Xanthomonas sp. strain 35Y (Gene bank accession code: AGT20506.1 - colocar link: http://www.ncbi.nlm.nih.gov/protein/AGT20506.1). false false _2244_ 21190 21190 9 false Because Xanthomonas sp. RoxA is exported to the extracellular environment, its open reading frame contains a sequence that codes to a signal peptide flanking its 5??? terminus. We detected this region (amino acid residues from 1 to 20) using signal peptide predictor server (4) (colocar link - http://www.compgen.org/tools/PRED-TAT) and eliminated it to generate this part with the coding sequence of RoxA flanked by SacI and NdeI restriction sites. Also, roxA contained a EcoRI restriction site. We perform directed mutagenesis PCR (for restriction site elimination) using a plasmid template for restriction site elimination false Lais Ribovski annotation2461813 1 ScaI site range2461813 1 1 6 annotation2461812 1 t-->c range2461812 1 124 124 annotation2461799 1 RoxA range2461799 1 7 1948 annotation2461824 1 NdeI site range2461824 1 1948 1954 BBa_K1819003_sequence 1 gagctcattgccactgctcgatcaggcatcgatccgctcgccgttgatggtgggctgcaatggtaagccggacagtacgccgctgccggtggatcctcgctcgctggtgaagcagggagtgaactccaatccaaacgcggcgcttcagttcaacgcttatttcgtcgacctgcacaatccaccgccgccgttcgtgaatcgtttgccgccacggccgacgacctgcggacagtttcgtgcgagcgccactcgcgggcgcgtcaatctcgaagagagacaattcttccagcccatggcgctggcgaccagttatcacttcatcttcctgcaatggggctatctgattcgcccacccgatttcgaagagcaggtgtcgaagcggtatggtttgtatcctgcgccattccgcaatccctacccgctgccgggcgaggatcccaatcaaaccaatggtggcagcggacaactgccgttgggcttgattcaaggcaaggatgacaacggtcggtggacgggcctgatcggcgcgtcgtgctcggcctgtcacgactcgcggctcggcaccgccagcgaagcatcgttcaagtggggcttgcccaacagcgcgaacgacgcgggcttgctggcgtcggatatgttccggaccacaccgatcacggcattgggcaatttgttacccattccgtggagcacgggtcgtggcagctcggacgcgatcggcttgatctcgctgctgccggcgctgttcgacatggaaaccctgaccctggcgccgagtctgctcgagtacgtcgccgatgccccgcacgcgggcatgaccaaggcgccggcctggtgggcgagagcgttcaagacgcgtcagttctgggacggctcgctgtcatcggacaacgtgcactcggaaatggcctttggcgttgcgaatatcttccgcgatgccaacgcgcgccgagggctcgaagacgagttcgaggacatcaacaacttcctgatctcgctgtcgccggcgacctatccaaaaaccatcaatacggcattggcggagcagggcgcggtcatctatcacgagcgcgacctgtgggcgagcggcgcgaacggggcgattcccaagccggccggcaacggctcatgcgcgagctgtcatggtgtctattcaccgcgtcacgcggcggatcccaactatctgccggatcctcgtttgaaaggggttgccgcggtcgtcacgccgatcgaaacgattcgtaccgatccgcgacgcatgcggctgatggccgatgagcgtcagcgccgcgcctggaacagtggctggtgggcgtacaacaatctcagccccagctggaccggctatccttcggacaacatcgtcgcgagcgaactgcgtcgcgtgccgcgtgccatttataacaatggtggaccgatctactcgccactcggacccaatatctgggaagagccgacgggttacatcgcgccgccgctgtatggcgcgtgggccacggctccgtatttccacaatggcagcgtgccgaatctgtggggcgtgctgaagccttcggatcgaccgaagctgtggaagcgtccgtacaccgcggcgggcatcggcggcaagaacgcgggctatgactacagtttcgcttcgtacgattggcagaagctgggctggaagtacacagcggtagcctgcaacaacagtatcttcacctcgccgttcctgccttgcacgcataacatggcgaccatcgatatcctgtactcgatgtgggacaacgtggcggctcagtatctgaacctggcgtatcagtcgcccccgccgatcacggatcagcagatcaagtcgcgcatggtctataacagctatctgtacggcaacgacaacggcggccacgacttcacccagtcgttgaccgactcggagcgttgggccctgatcgaatacatcaagacgttacatatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z