LOCUS       BBa_K1819004            2499 bp    DNA     linear       27-Jan-2026
DEFINITION  GFP+TetR+pTetR+mRFP1
ACCESSION   BBa_K1819004
VERSION     BBa_K1819004.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP protein
     CDS             1..720
                     /label=BBa_E0040
     misc_feature    733..736
                     /label=conserved
     RBS             729..740
                     /label=BBa_B0034
     CDS             750..1366
                     /label=tetR
     misc_feature    1367..1400
                     /label=SsrA
     misc_feature    1407..1431
                     /label=Help:Barcodes
     CDS             747..1406
                     /label=BBa_C0040
     stem_loop       1451..1494
                     /label=stem_loop
     misc_feature    1440..1519
                     /label=BBa_B0010
     terminator      1440..1519
                     /label=BBa_B0010
     stem_loop       1535..1554
                     /label=T7 TE
     misc_feature    1561..1561
                     /label=stop
     polyA_site      1555..1568
                     /label=polya
     misc_feature    1528..1568
                     /label=BBa_B0012
     terminator      1528..1568
                     /label=BBa_B0012
     misc_feature    729..1568
                     /label=BBa_P0440
     misc_feature    1577..1595
                     /label=TetR 1
     misc_feature    1577..1630
                     /label=BBa_R0040
     promoter        1596..1601
                     /label=-35
     misc_feature    1602..1620
                     /label=TetR 2
     promoter        1619..1624
                     /label=-10
     promoter        1577..1630
                     /label=BBa_R0040
     misc_feature    1643..1646
                     /label=conserved
     RBS             1639..1650
                     /label=BBa_B0034
     CDS             1657..2331
                     /label=mrfp1
     misc_feature    2338..2362
                     /label=Help:Barcodes
     CDS             1657..2337
                     /label=BBa_E1010
     stem_loop       2382..2425
                     /label=stem_loop
     misc_feature    2371..2450
                     /label=BBa_B0010
     terminator      2371..2450
                     /label=BBa_B0010
     stem_loop       2466..2485
                     /label=T7 TE
     misc_feature    2492..2492
                     /label=stop
     polyA_site      2486..2499
                     /label=polya
     misc_feature    2459..2499
                     /label=BBa_B0012
     terminator      2459..2499
                     /label=BBa_B0012
     misc_feature    1639..2499
                     /label=BBa_I13507
ORIGIN
        1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      721 tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa gtgattaaca
      781 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc
      841 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc
      901 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag
      961 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa
     1021 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa
     1081 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat
     1141 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc
     1201 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac
     1261 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat
     1321 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg
     1381 aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc
     1441 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1501 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1561 cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga
     1621 tactgagcac tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1681 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1741 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1801 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1861 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1921 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1981 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     2041 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     2101 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     2161 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     2221 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     2281 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     2341 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt
     2401 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2461 acactggctc accttcgggt gggcctttct gcgtttata
//