##gff-version 3
##sequence-region BBa_K1819004 1 2499
BBa_K1819004	.	CDS	1	720	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K1819004	.	CDS	1	720	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1819004	.	promoter	1577	1630	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1819004	.	promoter	1619	1624	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1819004	.	non_covalent_binding_site	1602	1620	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1819004	.	non_covalent_binding_site	1577	1595	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1819004	.	promoter	1596	1601	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1819004	.	engineered_region	1577	1630	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1819004	.	engineered_region	1639	2499	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K1819004	.	CDS	1657	2337	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K1819004	.	engineered_tag	2338	2362	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1819004	.	CDS	1657	2331	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1819004	.	terminator	2371	2450	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K1819004	.	engineered_region	2371	2450	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1819004	.	stem_loop	2382	2425	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1819004	.	terminator	2459	2499	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K1819004	.	stem_loop	2466	2485	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1819004	.	stop_codon	2492	2492	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1819004	.	engineered_region	2459	2499	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1819004	.	polyA_site	2486	2499	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1819004	.	ribosome_entry_site	1639	1650	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K1819004	.	conserved_region	1643	1646	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1819004	.	engineered_region	729	1568	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K1819004	.	terminator	1440	1519	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1819004	.	engineered_region	1440	1519	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1819004	.	stem_loop	1451	1494	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1819004	.	terminator	1528	1568	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1819004	.	stem_loop	1535	1554	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1819004	.	stop_codon	1561	1561	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1819004	.	engineered_region	1528	1568	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1819004	.	polyA_site	1555	1568	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1819004	.	CDS	747	1406	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1819004	.	tag	1367	1400	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1819004	.	engineered_tag	1407	1431	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1819004	.	CDS	750	1366	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1819004	.	ribosome_entry_site	729	740	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1819004	.	conserved_region	733	736	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1819004
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaat
tttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaact
acctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaa
cagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaact
acaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaaga
agatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaag
aatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaa
atactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcc
caacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatac
aaataataatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattaga
gctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattg
tattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcactttt
gccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcg
cgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagccttttta
tgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattgg
aagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagc
tatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaa
caacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccc
tatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatg
gcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcg
aaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgct
gccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccg
gactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccg
ttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacgg
tccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggt
gaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaac
cggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttga
acagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata