##gff-version 3
##sequence-region BBa_K1819008 1 2725
BBa_K1819008	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1819008	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1819008	.	engineered_region	955	1794	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K1819008	.	terminator	1754	1794	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1819008	.	stem_loop	1761	1780	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1819008	.	engineered_region	1754	1794	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1819008	.	polyA_site	1781	1794	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1819008	.	stop_codon	1787	1787	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1819008	.	terminator	1666	1745	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1819008	.	engineered_region	1666	1745	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1819008	.	stem_loop	1677	1720	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1819008	.	CDS	973	1632	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1819008	.	engineered_tag	1633	1657	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1819008	.	CDS	976	1592	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1819008	.	tag	1593	1626	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1819008	.	ribosome_entry_site	955	966	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1819008	.	conserved_region	959	962	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1819008	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1819008	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1819008	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1819008	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1819008	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1819008	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1819008	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1819008	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1819008	.	engineered_region	1865	2725	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K1819008	.	CDS	1883	2563	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K1819008	.	CDS	1883	2557	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1819008	.	engineered_tag	2564	2588	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1819008	.	terminator	2685	2725	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K1819008	.	stem_loop	2692	2711	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1819008	.	engineered_region	2685	2725	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1819008	.	polyA_site	2712	2725	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1819008	.	stop_codon	2718	2718	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1819008	.	ribosome_entry_site	1865	1876	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K1819008	.	conserved_region	1869	1872	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1819008	.	terminator	2597	2676	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K1819008	.	engineered_region	2597	2676	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1819008	.	stem_loop	2608	2651	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1819008	.	CDS	227	946	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K1819008	.	CDS	227	946	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1819008	.	promoter	1803	1856	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1819008	.	promoter	1845	1850	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1819008	.	promoter	1822	1827	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1819008	.	engineered_region	1803	1856	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1819008	.	non_covalent_binding_site	1828	1846	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1819008	.	non_covalent_binding_site	1803	1821	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K1819008
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcgtaaagg
agaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagt
ggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccat
ggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactt
tttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgt
gctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaaca
ttcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaa
agttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaatt
ggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaaga
gagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataata
ctagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatg
aggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgt
aaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaa
ggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaa
aagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaagg
tttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagag
catcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattat
ttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatg
tgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgata
gagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccga
agacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggt
gaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgctt
gggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaa
actgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggac
tcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgc
agaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaat
gcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctg
ccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaac
gtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttata