LOCUS       BBa_K1819009            1123 bp    DNA     linear       18-May-2024
DEFINITION  GFP expression under constitutive Bacillus promoter control
ACCESSION   BBa_K1819009
VERSION     BBa_K1819009.1  
FEATURES             Location/Qualifiers
     promoter        1..237
                     /label=promoter
     promoter        1..237
                     /label=BBa_K823003
     RBS             246..260
                     /label=RBS-1\Strong
     RBS             253..257
                     /label=RBS
     misc_feature    246..260
                     /label=BBa_B0030
     RBS             246..260
                     /label=BBa_B0030
     CDS             267..986
                     /label=GFP protein
     CDS             267..986
                     /label=BBa_E0040
     stem_loop       1006..1049
                     /label=stem_loop
     misc_feature    995..1074
                     /label=BBa_B0010
     terminator      995..1074
                     /label=BBa_B0010
     stem_loop       1090..1109
                     /label=T7 TE
     misc_feature    1116..1116
                     /label=stop
     polyA_site      1110..1123
                     /label=polya
     misc_feature    1083..1123
                     /label=BBa_B0012
     terminator      1083..1123
                     /label=BBa_B0012
     misc_feature    246..1123
                     /label=BBa_E0840
ORIGIN
        1 ggagttctga gaattggtat gccttataag tccaattaac agttgaaaac ctgcatagga
       61 gagctatgcg ggttttttat tttacataat gatacataat ttaccgaaac ttgcggaaca
      121 taattgagga atcatagaat tttgtcaaaa taattttatt gacaacgtct tattaacgtt
      181 gatataattt aaattttatt tgacaaaaat gggctcgtgt tgtacaataa atgtagttac
      241 tagagattaa agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg
      301 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      361 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      421 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
      481 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
      541 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
      601 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
      661 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
      721 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
      781 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
      841 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
      901 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
      961 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct
     1021 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1081 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//