BBa_K1820001
1
BBa_K1820001
P335-Φ31 inducible middle promoter + RBS
2015-09-15T11:00:00Z
2015-09-18T02:12:04Z
Add
Add
false
false
_2245_
25667
25667
9
false
Add
false
Thomas Overbeck
component2461174
1
BBa_B0034
component2461172
1
BBa_K1820000
annotation2461174
1
BBa_B0034
range2461174
1
254
265
annotation2461172
1
BBa_K1820000
range2461172
1
1
245
BBa_K1820000
1
p1997
P335-Φ31 inducible middle promoter
2015-09-15T11:00:00Z
2015-09-17T11:14:48Z
add
add
false
false
_2245_
25667
25667
9
false
add
false
Thomas Overbeck
annotation2466377
1
Transcription Start Site B
range2466377
1
185
185
annotation2466373
1
Transcription Start Site A
range2466373
1
144
144
annotation2466374
1
BamHI
range2466374
1
1
6
annotation2466376
1
-10 Region B
range2466376
1
172
177
annotation2466375
1
-10 Region A
range2466375
1
132
137
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1820000_sequence
1
ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgacggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaataaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgtactggtgc
BBa_K1820001_sequence
1
ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgacggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaataaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgtactggtgctactagagaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z