BBa_K1820012 1 BBa_K1820012 RBS + mCherry(Lr) + terminator 2015-09-15T11:00:00Z 2015-09-18T07:02:30Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen component2474893 1 BBa_B0034 component2474904 1 BBa_K1820007 annotation2474893 1 BBa_B0034 range2474893 1 1 12 annotation2474904 1 BBa_K1820007 range2474904 1 19 866 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1820008 1 BBa_K1820008 CP8 promoter 2015-09-15T11:00:00Z 2015-09-16T11:24:52Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen annotation2474690 1 cp8 range2474690 1 1 58 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1820017 1 BBa_K1820017 CP8_RBS_mCherry(Lr)_Terminator 2015-09-15T11:00:00Z 2015-09-17T12:30:09Z Add Add false false _2245_ 25685 25685 9 false Add false Chad Lawrence Nielsen component2463628 1 BBa_K1820012 component2463616 1 BBa_K1820008 annotation2463628 1 BBa_K1820012 range2463628 1 69 934 annotation2463616 1 BBa_K1820008 range2463616 1 1 60 BBa_K1820007 1 BBa_K1820007 mCherry(Lr) + terminator 2015-09-15T11:00:00Z 2015-09-18T06:05:40Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen component2474591 1 BBa_B0015 component2474584 1 BBa_K1820005 annotation2474584 1 BBa_K1820005 range2474584 1 1 711 annotation2474591 1 BBa_B0015 range2474591 1 720 848 BBa_K1820005 1 BBa_K1820005 mCherry(Lr) 2015-09-15T11:00:00Z 2015-09-18T08:17:16Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen annotation2474461 1 mCherry range2474461 1 4 708 annotation2475183 1 stop codon range2475183 1 708 711 annotation2474460 1 start codon range2474460 1 1 3 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1820012_sequence 1 aaagaggagaaatactagatggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1820005_sequence 1 atggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataa BBa_K1820007_sequence 1 atggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1820017_sequence 1 cattctttagtttattcttgacaaacgtattgaggactgatataataggtgagtactgtttactagagaaagaggagaaatactagatggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1820008_sequence 1 cattctttagtttattcttgacaaacgtattgaggactgatataataggtgagtactgtt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z