##gff-version 3
##sequence-region BBa_K1820020 1 1125
BBa_K1820020	.	engineered_region	272	1125	.	+	0	ID=BBa_K1820006;Name=BBa_K1820006
BBa_K1820020	.	engineered_region	272	988	.	+	0	ID=BBa_K1820004;Name=BBa_K1820004;Parent=BBa_K1820006
BBa_K1820020	.	stop_codon	985	988	.	+	0	ID=annotation2475184;Name=stop codon;Parent=BBa_K1820004
BBa_K1820020	.	CDS	275	985	.	+	0	ID=annotation2474464;Name=sfGFP;Parent=BBa_K1820004
BBa_K1820020	.	start_codon	272	274	.	+	0	ID=annotation2474463;Name=start codon;Parent=BBa_K1820004
BBa_K1820020	.	terminator	997	1125	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1820006
BBa_K1820020	.	terminator	1085	1125	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1820020	.	polyA_site	1112	1125	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1820020	.	engineered_region	1085	1125	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1820020	.	stem_loop	1092	1111	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1820020	.	stop_codon	1118	1118	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1820020	.	terminator	997	1076	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1820020	.	stem_loop	1008	1051	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1820020	.	engineered_region	997	1076	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1820020	.	engineered_region	1	265	.	+	0	ID=BBa_K1820001;Name=BBa_K1820001
BBa_K1820020	.	ribosome_entry_site	254	265	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1820001
BBa_K1820020	.	conserved_region	258	261	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1820020	.	promoter	1	245	.	+	0	ID=BBa_K1820000;Name=BBa_K1820000;Parent=BBa_K1820001
BBa_K1820020	.	non_covalent_binding_site	172	177	.	+	0	ID=annotation2466376;Name=-10 Region B;Parent=BBa_K1820000
BBa_K1820020	.	non_covalent_binding_site	132	137	.	+	0	ID=annotation2466375;Name=-10 Region A;Parent=BBa_K1820000
BBa_K1820020	.	sequence_feature	185	185	.	+	0	ID=annotation2466377;Name=Transcription Start Site B;Parent=BBa_K1820000
BBa_K1820020	.	null	1	6	.	+	0	ID=annotation2466374;Name=BamHI;Parent=BBa_K1820000
BBa_K1820020	.	sequence_feature	144	144	.	+	0	ID=annotation2466373;Name=Transcription Start Site A;Parent=BBa_K1820000
>BBa_K1820020
ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgac
ggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaa
taaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgt
actggtgctactagagaaagaggagaaatactagatgtcaaaaggagaagagctgttcacaggtgttgtgccgattctc
gttgagcttgacggagatgtaaacggacacaaattctctgttcgcggtgaaggtgaaggagatgcaacaaacggcaagc
tgacattgaagtttatttgcacaactggaaagctgccggttccttggccgacacttgtaacgacgctgacttacggcgt
tcaatgcttctctcgttatccagaccacatgaaacgccatgatttcttcaaatctgcaatgcctgaaggctacgttcaa
gagcgtacgatcagcttcaaagatgacggaacgtacaaaacaagagcagaagtgaagtttgaaggtgacacacttgtga
accgcattgaattgaaaggcattgatttcaaagaagatggaaacatccttggacacaaacttgaatacaacttcaacag
ccacaacgtatacatcactgctgacaaacaaaaaaacggcatcaaagcaaacttcaaaatccgtcataacgtagaggac
ggttctgttcagcttgctgatcattatcagcaaaatacaccgatcggtgacggcccggttcttcttcctgataaccatt
atttatcaactcaaagcgtattatcaaaagacccaaatgaaaagcgtgaccacatggtgctgcttgaatttgtgacagc
tgctggtatcactcacggcatggatgagctttataagtaatactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttata