LOCUS       BBa_K1820021            1119 bp    DNA     linear       10-Jun-2024
DEFINITION  P335-Φ31 inducible middle promoter + RBS + mCherry + term
ACCESSION   BBa_K1820021
VERSION     BBa_K1820021.1  
FEATURES             Location/Qualifiers
     misc_feature    144..144
                     /label=Transcription Start Site A
     misc_feature    1..6
                     /label=BamHI
     misc_feature    132..137
                     /label=-10 Region A
     misc_feature    172..177
                     /label=-10 Region B
     misc_feature    185..185
                     /label=Transcription Start Site B
     promoter        1..245
                     /label=BBa_K1820000
     misc_feature    258..261
                     /label=conserved
     RBS             254..265
                     /label=BBa_B0034
     misc_feature    1..265
                     /label=BBa_K1820001
     misc_feature    272..274
                     /label=start codon
     CDS             275..979
                     /label=mCherry
     misc_feature    979..982
                     /label=stop codon
     misc_feature    272..982
                     /label=BBa_K1820005
     stem_loop       1002..1045
                     /label=stem_loop
     misc_feature    991..1070
                     /label=BBa_B0010
     terminator      991..1070
                     /label=BBa_B0010
     stem_loop       1086..1105
                     /label=T7 TE
     misc_feature    1112..1112
                     /label=stop
     polyA_site      1106..1119
                     /label=polya
     misc_feature    1079..1119
                     /label=BBa_B0012
     terminator      1079..1119
                     /label=BBa_B0012
     terminator      991..1119
                     /label=BBa_B0015
     misc_feature    272..1119
                     /label=BBa_K1820007
ORIGIN
        1 ggatccgtgt cacataactg agcgccaata ctttagaaag agaagagcaa tacttgagaa
       61 gtatgatgag atatgtgacg gcttctggta atttgtcacc ttttgggcga aaactgacaa
      121 gataaatgtt gtattatagt atcatcaaat aaaacaaata aagccagcgg atatattctg
      181 ttggcttttt gtgtggagaa agtgaggtga cctcccatag cattacgtgc tgaccgtact
      241 ggtgctacta gagaaagagg agaaatacta gatggtttct aagggtgaag aagataatat
      301 ggctattatt aaggaattta tgcgttttaa ggtacatatg gaaggtagtg ttaatggtca
      361 tgaatttgaa attgaaggtg aaggagaagg tcggccatat gaaggtactc aaactgctaa
      421 gttaaaggtt actaaaggtg gaccattacc ttttgcatgg gatattcttt caccacaatt
      481 tatgtacgga tctaaggctt atgttaagca cccagcagat attcctgatt accttaaact
      541 tagttttcct gaaggtttta agtgggaacg agttatgaat tttgaagatg gtggagttgt
      601 aactgtaaca caagatagtt cattacaaga tggagaattt atttacaagg ttaagttacg
      661 tggaactaat tttccatcag atggtcctgt aatgcaaaag aaaactatgg gttgggaagc
      721 tagtagtgaa cgtatgtacc cagaagatgg agcattaaag ggtgaaatta agcaacggct
      781 taaacttaag gatggtggac attacgatgc tgaagttaag actacataca aggcaaaaaa
      841 gccagttcaa ttacctggtg cttacaatgt aaatattaag ttagatatta cttctcacaa
      901 tgaagattac acaattgttg aacaatacga acgggcagaa ggtcggcact ctactggagg
      961 aatggatgaa ctttacaaat aatactagag ccaggcatca aataaaacga aaggctcagt
     1021 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1081 acactggctc accttcgggt gggcctttct gcgtttata
//