BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1820000 1 p1997 P335-&#934;31 inducible middle promoter 2015-09-15T11:00:00Z 2015-09-17T11:14:48Z add add false false _2245_ 25667 25667 9 false add false Thomas Overbeck annotation2466373 1 Transcription Start Site A range2466373 1 144 144 annotation2466374 1 BamHI range2466374 1 1 6 annotation2466377 1 Transcription Start Site B range2466377 1 185 185 annotation2466376 1 -10 Region B range2466376 1 172 177 annotation2466375 1 -10 Region A range2466375 1 132 137 BBa_K1820001 1 BBa_K1820001 P335-&#934;31 inducible middle promoter + RBS 2015-09-15T11:00:00Z 2015-09-18T02:12:04Z Add Add false false _2245_ 25667 25667 9 false Add false Thomas Overbeck component2461174 1 BBa_B0034 component2461172 1 BBa_K1820000 annotation2461174 1 BBa_B0034 range2461174 1 254 265 annotation2461172 1 BBa_K1820000 range2461172 1 1 245 BBa_K1820007 1 BBa_K1820007 mCherry(Lr) + terminator 2015-09-15T11:00:00Z 2015-09-18T06:05:40Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen component2474591 1 BBa_B0015 component2474584 1 BBa_K1820005 annotation2474584 1 BBa_K1820005 range2474584 1 1 711 annotation2474591 1 BBa_B0015 range2474591 1 720 848 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1820021 1 BBa_K1820021 P335-&#934;31 inducible middle promoter + RBS + mCherry + term 2015-09-15T11:00:00Z 2015-09-18T07:14:07Z Add Add false false _1796_ 25667 22545 9 false Add false Sara Gertsch component2461684 1 BBa_K1820001 component2461693 1 BBa_K1820007 annotation2461684 1 BBa_K1820001 range2461684 1 1 265 annotation2461693 1 BBa_K1820007 range2461693 1 272 1119 BBa_K1820005 1 BBa_K1820005 mCherry(Lr) 2015-09-15T11:00:00Z 2015-09-18T08:17:16Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen annotation2475183 1 stop codon range2475183 1 708 711 annotation2474461 1 mCherry range2474461 1 4 708 annotation2474460 1 start codon range2474460 1 1 3 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1820021_sequence 1 ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgacggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaataaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgtactggtgctactagagaaagaggagaaatactagatggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K1820000_sequence 1 ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgacggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaataaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgtactggtgc BBa_K1820001_sequence 1 ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgacggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaataaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgtactggtgctactagagaaagaggagaaa BBa_K1820005_sequence 1 atggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataa BBa_K1820007_sequence 1 atggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z