LOCUS       BBa_K1820024            1160 bp    DNA     linear       22-Dec-2024
DEFINITION  Phage inducible promoter 1997 +RBS+FspI + term
ACCESSION   BBa_K1820024
VERSION     BBa_K1820024.1  
FEATURES             Location/Qualifiers
     misc_feature    144..144
                     /label=Transcription Start Site A
     misc_feature    1..6
                     /label=BamHI
     misc_feature    132..137
                     /label=-10 Region A
     misc_feature    172..177
                     /label=-10 Region B
     misc_feature    185..185
                     /label=Transcription Start Site B
     promoter        1..245
                     /label=BBa_K1820000
     misc_feature    258..261
                     /label=conserved
     RBS             254..265
                     /label=BBa_B0034
     misc_feature    1..265
                     /label=BBa_K1820001
     CDS             274..1020
                     /label=FspI
     misc_feature    1020..1023
                     /label=stop codon
     CDS             274..1023
                     /label=BBa_K1820022
     stem_loop       1043..1086
                     /label=stem_loop
     misc_feature    1032..1111
                     /label=BBa_B0010
     terminator      1032..1111
                     /label=BBa_B0010
     stem_loop       1127..1146
                     /label=T7 TE
     misc_feature    1153..1153
                     /label=stop
     polyA_site      1147..1160
                     /label=polya
     misc_feature    1120..1160
                     /label=BBa_B0012
     terminator      1120..1160
                     /label=BBa_B0012
     terminator      1032..1160
                     /label=BBa_B0015
     misc_feature    274..1160
                     /label=BBa_K1820023
ORIGIN
        1 ggatccgtgt cacataactg agcgccaata ctttagaaag agaagagcaa tacttgagaa
       61 gtatgatgag atatgtgacg gcttctggta atttgtcacc ttttgggcga aaactgacaa
      121 gataaatgtt gtattatagt atcatcaaat aaaacaaata aagccagcgg atatattctg
      181 ttggcttttt gtgtggagaa agtgaggtga cctcccatag cattacgtgc tgaccgtact
      241 ggtgctacta gagaaagagg agaaatacta gaggtgttga caaacaacga gattgaaaga
      301 ttaaggcaag ccattatcgc gaccattgca tctcctgtaa ttggctcgat agaagattat
      361 acatgggaag caatttttca ttatgttaag gatattcctt tatcagatcc cgctctggga
      421 cgcagcaagc ttctctatga tgctgttgac gtagttacta aaactggttg gtcactcaaa
      481 tccctccaat tgaagagcct taactttaaa agcccatttt tatttgttat tcagagagca
      541 gatatcctta agaagtctgt ccagctgggt tttcctggtc tgactgagca atcttcgccg
      601 gatgagcttg gagcagccat tatccaacat tggaatgaga agattatttt gagtcaggca
      661 gcacaaagcg ttgtaaatag ttatgaaggc atattactga aaactatcaa aggttacgag
      721 tatatctatt gtgagtttcc actcgatcct cttgatccaa gcacgttttc ttgggcttgg
      781 acggtggaca aaactactgg cggtgcaggg gtagggctac aaggtagcat tgtgggcaaa
      841 acagaattag tgtggtataa aaatcagaaa caacttttca gagctaggac tattcccgca
      901 caagcggttc gtattacagt tgaaagaact cgtcttactc ttgatcgata tgtaaagaca
      961 gttatctttg ctttgcaaga tcaaatcaac atgcagtttt ctgagaatga gcctgaagaa
     1021 tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1081 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1141 tgggcctttc tgcgtttata
//