BBa_K1820000
1
p1997
P335-Φ31 inducible middle promoter
2015-09-15T11:00:00Z
2015-09-17T11:14:48Z
add
add
false
false
_2245_
25667
25667
9
false
add
false
Thomas Overbeck
annotation2466377
1
Transcription Start Site B
range2466377
1
185
185
annotation2466373
1
Transcription Start Site A
range2466373
1
144
144
annotation2466376
1
-10 Region B
range2466376
1
172
177
annotation2466375
1
-10 Region A
range2466375
1
132
137
annotation2466374
1
BamHI
range2466374
1
1
6
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1820024
1
BBa_K1820024
Phage inducible promoter 1997 +RBS+FspI + term
2015-09-15T11:00:00Z
2015-09-16T03:22:20Z
Add
Add
false
false
_2245_
25685
25685
9
false
Add
false
Chad Lawrence Nielsen
component2462202
1
BBa_K1820001
component2462211
1
BBa_K1820023
annotation2462211
1
BBa_K1820023
range2462211
1
274
1160
annotation2462202
1
BBa_K1820001
range2462202
1
1
265
BBa_K1820001
1
BBa_K1820001
P335-Φ31 inducible middle promoter + RBS
2015-09-15T11:00:00Z
2015-09-18T02:12:04Z
Add
Add
false
false
_2245_
25667
25667
9
false
Add
false
Thomas Overbeck
component2461172
1
BBa_K1820000
component2461174
1
BBa_B0034
annotation2461172
1
BBa_K1820000
range2461172
1
1
245
annotation2461174
1
BBa_B0034
range2461174
1
254
265
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_K1820022
1
BBa_K1820022
FspI restriction enzyme
2015-09-15T11:00:00Z
2015-09-18T09:24:07Z
Add
Add
false
false
_1796_
25686
22545
9
false
Add
false
Sara Gertsch
annotation2475324
1
stop codon
range2475324
1
747
750
annotation2475323
1
FspI
range2475323
1
1
747
BBa_K1820023
1
BBa_K1820023
FspI + terminator
2015-09-15T11:00:00Z
2015-09-18T08:21:15Z
Add
Add
false
false
_2245_
25685
25685
9
false
Add
false
Chad Lawrence Nielsen
component2462191
1
BBa_K1820022
component2462198
1
BBa_B0015
annotation2462198
1
BBa_B0015
range2462198
1
759
887
annotation2462191
1
BBa_K1820022
range2462191
1
1
750
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1820022_sequence
1
gtgttgacaaacaacgagattgaaagattaaggcaagccattatcgcgaccattgcatctcctgtaattggctcgatagaagattatacatgggaagcaatttttcattatgttaaggatattcctttatcagatcccgctctgggacgcagcaagcttctctatgatgctgttgacgtagttactaaaactggttggtcactcaaatccctccaattgaagagccttaactttaaaagcccatttttatttgttattcagagagcagatatccttaagaagtctgtccagctgggttttcctggtctgactgagcaatcttcgccggatgagcttggagcagccattatccaacattggaatgagaagattattttgagtcaggcagcacaaagcgttgtaaatagttatgaaggcatattactgaaaactatcaaaggttacgagtatatctattgtgagtttccactcgatcctcttgatccaagcacgttttcttgggcttggacggtggacaaaactactggcggtgcaggggtagggctacaaggtagcattgtgggcaaaacagaattagtgtggtataaaaatcagaaacaacttttcagagctaggactattcccgcacaagcggttcgtattacagttgaaagaactcgtcttactcttgatcgatatgtaaagacagttatctttgctttgcaagatcaaatcaacatgcagttttctgagaatgagcctgaagaatag
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1820023_sequence
1
gtgttgacaaacaacgagattgaaagattaaggcaagccattatcgcgaccattgcatctcctgtaattggctcgatagaagattatacatgggaagcaatttttcattatgttaaggatattcctttatcagatcccgctctgggacgcagcaagcttctctatgatgctgttgacgtagttactaaaactggttggtcactcaaatccctccaattgaagagccttaactttaaaagcccatttttatttgttattcagagagcagatatccttaagaagtctgtccagctgggttttcctggtctgactgagcaatcttcgccggatgagcttggagcagccattatccaacattggaatgagaagattattttgagtcaggcagcacaaagcgttgtaaatagttatgaaggcatattactgaaaactatcaaaggttacgagtatatctattgtgagtttccactcgatcctcttgatccaagcacgttttcttgggcttggacggtggacaaaactactggcggtgcaggggtagggctacaaggtagcattgtgggcaaaacagaattagtgtggtataaaaatcagaaacaacttttcagagctaggactattcccgcacaagcggttcgtattacagttgaaagaactcgtcttactcttgatcgatatgtaaagacagttatctttgctttgcaagatcaaatcaacatgcagttttctgagaatgagcctgaagaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1820000_sequence
1
ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgacggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaataaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgtactggtgc
BBa_K1820001_sequence
1
ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgacggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaataaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgtactggtgctactagagaaagaggagaaa
BBa_K1820024_sequence
1
ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgacggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaataaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgtactggtgctactagagaaagaggagaaatactagaggtgttgacaaacaacgagattgaaagattaaggcaagccattatcgcgaccattgcatctcctgtaattggctcgatagaagattatacatgggaagcaatttttcattatgttaaggatattcctttatcagatcccgctctgggacgcagcaagcttctctatgatgctgttgacgtagttactaaaactggttggtcactcaaatccctccaattgaagagccttaactttaaaagcccatttttatttgttattcagagagcagatatccttaagaagtctgtccagctgggttttcctggtctgactgagcaatcttcgccggatgagcttggagcagccattatccaacattggaatgagaagattattttgagtcaggcagcacaaagcgttgtaaatagttatgaaggcatattactgaaaactatcaaaggttacgagtatatctattgtgagtttccactcgatcctcttgatccaagcacgttttcttgggcttggacggtggacaaaactactggcggtgcaggggtagggctacaaggtagcattgtgggcaaaacagaattagtgtggtataaaaatcagaaacaacttttcagagctaggactattcccgcacaagcggttcgtattacagttgaaagaactcgtcttactcttgatcgatatgtaaagacagttatctttgctttgcaagatcaaatcaacatgcagttttctgagaatgagcctgaagaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z