##gff-version 3
##sequence-region BBa_K1820024 1 1160
BBa_K1820024	.	engineered_region	274	1160	.	+	0	ID=BBa_K1820023;Name=BBa_K1820023
BBa_K1820024	.	CDS	274	1023	.	+	0	ID=BBa_K1820022;Name=BBa_K1820022;Parent=BBa_K1820023
BBa_K1820024	.	stop_codon	1020	1023	.	+	0	ID=annotation2475324;Name=stop codon;Parent=BBa_K1820022
BBa_K1820024	.	CDS	274	1020	.	+	0	ID=annotation2475323;Name=FspI;Parent=BBa_K1820022
BBa_K1820024	.	terminator	1032	1160	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1820023
BBa_K1820024	.	terminator	1032	1111	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1820024	.	engineered_region	1032	1111	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1820024	.	stem_loop	1043	1086	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1820024	.	terminator	1120	1160	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1820024	.	stop_codon	1153	1153	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1820024	.	polyA_site	1147	1160	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1820024	.	stem_loop	1127	1146	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1820024	.	engineered_region	1120	1160	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1820024	.	engineered_region	1	265	.	+	0	ID=BBa_K1820001;Name=BBa_K1820001
BBa_K1820024	.	promoter	1	245	.	+	0	ID=BBa_K1820000;Name=BBa_K1820000;Parent=BBa_K1820001
BBa_K1820024	.	sequence_feature	185	185	.	+	0	ID=annotation2466377;Name=Transcription Start Site B;Parent=BBa_K1820000
BBa_K1820024	.	sequence_feature	144	144	.	+	0	ID=annotation2466373;Name=Transcription Start Site A;Parent=BBa_K1820000
BBa_K1820024	.	non_covalent_binding_site	172	177	.	+	0	ID=annotation2466376;Name=-10 Region B;Parent=BBa_K1820000
BBa_K1820024	.	null	1	6	.	+	0	ID=annotation2466374;Name=BamHI;Parent=BBa_K1820000
BBa_K1820024	.	non_covalent_binding_site	132	137	.	+	0	ID=annotation2466375;Name=-10 Region A;Parent=BBa_K1820000
BBa_K1820024	.	ribosome_entry_site	254	265	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1820001
BBa_K1820024	.	conserved_region	258	261	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1820024
ggatccgtgtcacataactgagcgccaatactttagaaagagaagagcaatacttgagaagtatgatgagatatgtgac
ggcttctggtaatttgtcaccttttgggcgaaaactgacaagataaatgttgtattatagtatcatcaaataaaacaaa
taaagccagcggatatattctgttggctttttgtgtggagaaagtgaggtgacctcccatagcattacgtgctgaccgt
actggtgctactagagaaagaggagaaatactagaggtgttgacaaacaacgagattgaaagattaaggcaagccatta
tcgcgaccattgcatctcctgtaattggctcgatagaagattatacatgggaagcaatttttcattatgttaaggatat
tcctttatcagatcccgctctgggacgcagcaagcttctctatgatgctgttgacgtagttactaaaactggttggtca
ctcaaatccctccaattgaagagccttaactttaaaagcccatttttatttgttattcagagagcagatatccttaaga
agtctgtccagctgggttttcctggtctgactgagcaatcttcgccggatgagcttggagcagccattatccaacattg
gaatgagaagattattttgagtcaggcagcacaaagcgttgtaaatagttatgaaggcatattactgaaaactatcaaa
ggttacgagtatatctattgtgagtttccactcgatcctcttgatccaagcacgttttcttgggcttggacggtggaca
aaactactggcggtgcaggggtagggctacaaggtagcattgtgggcaaaacagaattagtgtggtataaaaatcagaa
acaacttttcagagctaggactattcccgcacaagcggttcgtattacagttgaaagaactcgtcttactcttgatcga
tatgtaaagacagttatctttgctttgcaagatcaaatcaacatgcagttttctgagaatgagcctgaagaatagtact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata