##gff-version 3
##sequence-region BBa_K1820025 1 1209
BBa_K1820025	.	engineered_region	323	1209	.	+	0	ID=BBa_K1820023;Name=BBa_K1820023
BBa_K1820025	.	terminator	1081	1209	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1820023
BBa_K1820025	.	terminator	1081	1160	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1820025	.	engineered_region	1081	1160	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1820025	.	stem_loop	1092	1135	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1820025	.	terminator	1169	1209	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1820025	.	engineered_region	1169	1209	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1820025	.	stop_codon	1202	1202	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1820025	.	polyA_site	1196	1209	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1820025	.	stem_loop	1176	1195	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1820025	.	CDS	323	1072	.	+	0	ID=BBa_K1820022;Name=BBa_K1820022;Parent=BBa_K1820023
BBa_K1820025	.	CDS	323	1069	.	+	0	ID=annotation2475323;Name=FspI;Parent=BBa_K1820022
BBa_K1820025	.	stop_codon	1069	1072	.	+	0	ID=annotation2475324;Name=stop codon;Parent=BBa_K1820022
BBa_K1820025	.	promoter	1	314	.	+	0	ID=BBa_K1820003;Name=BBa_K1820003
BBa_K1820025	.	non_covalent_binding_site	140	145	.	+	0	ID=annotation2466356;Name=Rightward -10;Parent=BBa_K1820003
BBa_K1820025	.	non_covalent_binding_site	71	76	.	+	0	ID=annotation2466351;Name=Leftward -10;Parent=BBa_K1820003
BBa_K1820025	.	promoter	71	99	.	+	0	ID=annotation2466350;Name=Leftward Promoter;Parent=BBa_K1820003
BBa_K1820025	.	non_covalent_binding_site	118	123	.	+	0	ID=annotation2466355;Name=Rightward -35;Parent=BBa_K1820003
BBa_K1820025	.	operator	88	115	.	+	0	ID=annotation2466352;Name=O1;Parent=BBa_K1820003
BBa_K1820025	.	non_covalent_binding_site	94	99	.	+	0	ID=annotation2466353;Name=Leftward -35;Parent=BBa_K1820003
BBa_K1820025	.	promoter	118	145	.	+	0	ID=annotation2466354;Name=Rightward Promoter;Parent=BBa_K1820003
BBa_K1820025	.	ribosome_entry_site	304	312	.	+	0	ID=annotation2466359;Name=Native RBS;Parent=BBa_K1820003
BBa_K1820025	.	sequence_feature	153	153	.	+	0	ID=annotation2466358;Name=Transcriptional Start Site;Parent=BBa_K1820003
BBa_K1820025	.	operator	152	180	.	+	0	ID=annotation2466357;Name=O2;Parent=BBa_K1820003
>BBa_K1820025
cctgtcttaatcctcggtcgttcattattttctttaatctcatttgagagttttcttttttcatgaacttattataaaa
acatttataaaaaagttcaaactttttatactttttccttgacaattattttccaaatgatataattaaatcataaagt
tcaaaacatttgaactttatatcaaacaagcgaacaatcatggagcattcagtacggcagacggaacaggctcaaatga
cggtacacgacgtatccaccgcgacgtaagtagcaagtttggcaaataaaaagccccagaggggcagaaaggatgtata
ctagaggtgttgacaaacaacgagattgaaagattaaggcaagccattatcgcgaccattgcatctcctgtaattggct
cgatagaagattatacatgggaagcaatttttcattatgttaaggatattcctttatcagatcccgctctgggacgcag
caagcttctctatgatgctgttgacgtagttactaaaactggttggtcactcaaatccctccaattgaagagccttaac
tttaaaagcccatttttatttgttattcagagagcagatatccttaagaagtctgtccagctgggttttcctggtctga
ctgagcaatcttcgccggatgagcttggagcagccattatccaacattggaatgagaagattattttgagtcaggcagc
acaaagcgttgtaaatagttatgaaggcatattactgaaaactatcaaaggttacgagtatatctattgtgagtttcca
ctcgatcctcttgatccaagcacgttttcttgggcttggacggtggacaaaactactggcggtgcaggggtagggctac
aaggtagcattgtgggcaaaacagaattagtgtggtataaaaatcagaaacaacttttcagagctaggactattcccgc
acaagcggttcgtattacagttgaaagaactcgtcttactcttgatcgatatgtaaagacagttatctttgctttgcaa
gatcaaatcaacatgcagttttctgagaatgagcctgaagaatagtactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata