BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1820023 1 BBa_K1820023 FspI + terminator 2015-09-15T11:00:00Z 2015-09-18T08:21:15Z Add Add false false _2245_ 25685 25685 9 false Add false Chad Lawrence Nielsen component2462198 1 BBa_B0015 component2462191 1 BBa_K1820022 annotation2462191 1 BBa_K1820022 range2462191 1 1 750 annotation2462198 1 BBa_B0015 range2462198 1 759 887 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1820022 1 BBa_K1820022 FspI restriction enzyme 2015-09-15T11:00:00Z 2015-09-18T09:24:07Z Add Add false false _1796_ 25686 22545 9 false Add false Sara Gertsch annotation2475324 1 stop codon range2475324 1 747 750 annotation2475323 1 FspI range2475323 1 1 747 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1820025 1 BBa_K1820025 p2001-native RBS-Fsp1-B0015 2015-09-15T11:00:00Z 2015-09-16T03:31:02Z Add Add false false _2245_ 25685 25685 9 false Add false Chad Lawrence Nielsen component2462221 1 BBa_K1820023 component2462212 1 BBa_K1820003 annotation2462221 1 BBa_K1820023 range2462221 1 323 1209 annotation2462212 1 BBa_K1820003 range2462212 1 1 314 BBa_K1820003 1 BBa_K1820003 P335-&#934;31phage genetic switch-divergent promoter + native RBS 2015-09-15T11:00:00Z 2015-09-17T10:20:40Z Add Add false false _2245_ 25667 25667 9 false Add false Thomas Overbeck annotation2466356 1 Rightward -10 range2466356 1 140 145 annotation2466355 1 Rightward -35 range2466355 1 118 123 annotation2466354 1 Rightward Promoter range2466354 1 118 145 annotation2466359 1 Native RBS range2466359 1 304 312 annotation2466350 1 Leftward Promoter range2466350 1 71 99 annotation2466351 1 Leftward -10 range2466351 1 71 76 annotation2466352 1 O1 range2466352 1 88 115 annotation2466353 1 Leftward -35 range2466353 1 94 99 annotation2466358 1 Transcriptional Start Site range2466358 1 153 153 annotation2466357 1 O2 range2466357 1 152 180 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1820022_sequence 1 gtgttgacaaacaacgagattgaaagattaaggcaagccattatcgcgaccattgcatctcctgtaattggctcgatagaagattatacatgggaagcaatttttcattatgttaaggatattcctttatcagatcccgctctgggacgcagcaagcttctctatgatgctgttgacgtagttactaaaactggttggtcactcaaatccctccaattgaagagccttaactttaaaagcccatttttatttgttattcagagagcagatatccttaagaagtctgtccagctgggttttcctggtctgactgagcaatcttcgccggatgagcttggagcagccattatccaacattggaatgagaagattattttgagtcaggcagcacaaagcgttgtaaatagttatgaaggcatattactgaaaactatcaaaggttacgagtatatctattgtgagtttccactcgatcctcttgatccaagcacgttttcttgggcttggacggtggacaaaactactggcggtgcaggggtagggctacaaggtagcattgtgggcaaaacagaattagtgtggtataaaaatcagaaacaacttttcagagctaggactattcccgcacaagcggttcgtattacagttgaaagaactcgtcttactcttgatcgatatgtaaagacagttatctttgctttgcaagatcaaatcaacatgcagttttctgagaatgagcctgaagaatag BBa_K1820003_sequence 1 cctgtcttaatcctcggtcgttcattattttctttaatctcatttgagagttttcttttttcatgaacttattataaaaacatttataaaaaagttcaaactttttatactttttccttgacaattattttccaaatgatataattaaatcataaagttcaaaacatttgaactttatatcaaacaagcgaacaatcatggagcattcagtacggcagacggaacaggctcaaatgacggtacacgacgtatccaccgcgacgtaagtagcaagtttggcaaataaaaagccccagaggggcagaaaggatgta BBa_K1820023_sequence 1 gtgttgacaaacaacgagattgaaagattaaggcaagccattatcgcgaccattgcatctcctgtaattggctcgatagaagattatacatgggaagcaatttttcattatgttaaggatattcctttatcagatcccgctctgggacgcagcaagcttctctatgatgctgttgacgtagttactaaaactggttggtcactcaaatccctccaattgaagagccttaactttaaaagcccatttttatttgttattcagagagcagatatccttaagaagtctgtccagctgggttttcctggtctgactgagcaatcttcgccggatgagcttggagcagccattatccaacattggaatgagaagattattttgagtcaggcagcacaaagcgttgtaaatagttatgaaggcatattactgaaaactatcaaaggttacgagtatatctattgtgagtttccactcgatcctcttgatccaagcacgttttcttgggcttggacggtggacaaaactactggcggtgcaggggtagggctacaaggtagcattgtgggcaaaacagaattagtgtggtataaaaatcagaaacaacttttcagagctaggactattcccgcacaagcggttcgtattacagttgaaagaactcgtcttactcttgatcgatatgtaaagacagttatctttgctttgcaagatcaaatcaacatgcagttttctgagaatgagcctgaagaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1820025_sequence 1 cctgtcttaatcctcggtcgttcattattttctttaatctcatttgagagttttcttttttcatgaacttattataaaaacatttataaaaaagttcaaactttttatactttttccttgacaattattttccaaatgatataattaaatcataaagttcaaaacatttgaactttatatcaaacaagcgaacaatcatggagcattcagtacggcagacggaacaggctcaaatgacggtacacgacgtatccaccgcgacgtaagtagcaagtttggcaaataaaaagccccagaggggcagaaaggatgtatactagaggtgttgacaaacaacgagattgaaagattaaggcaagccattatcgcgaccattgcatctcctgtaattggctcgatagaagattatacatgggaagcaatttttcattatgttaaggatattcctttatcagatcccgctctgggacgcagcaagcttctctatgatgctgttgacgtagttactaaaactggttggtcactcaaatccctccaattgaagagccttaactttaaaagcccatttttatttgttattcagagagcagatatccttaagaagtctgtccagctgggttttcctggtctgactgagcaatcttcgccggatgagcttggagcagccattatccaacattggaatgagaagattattttgagtcaggcagcacaaagcgttgtaaatagttatgaaggcatattactgaaaactatcaaaggttacgagtatatctattgtgagtttccactcgatcctcttgatccaagcacgttttcttgggcttggacggtggacaaaactactggcggtgcaggggtagggctacaaggtagcattgtgggcaaaacagaattagtgtggtataaaaatcagaaacaacttttcagagctaggactattcccgcacaagcggttcgtattacagttgaaagaactcgtcttactcttgatcgatatgtaaagacagttatctttgctttgcaagatcaaatcaacatgcagttttctgagaatgagcctgaagaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z