LOCUS       BBa_K1820026            1174 bp    DNA     linear       22-Nov-2024
DEFINITION  p2001-native RBS-sfGFP-B0015
ACCESSION   BBa_K1820026
VERSION     BBa_K1820026.1  
FEATURES             Location/Qualifiers
     promoter        71..99
                     /label=Leftward Promoter
     misc_feature    71..76
                     /label=Leftward -10
     misc_feature    88..115
                     /label=O1
     misc_feature    94..99
                     /label=Leftward -35
     promoter        118..145
                     /label=Rightward Promoter
     misc_feature    118..123
                     /label=Rightward -35
     misc_feature    140..145
                     /label=Rightward -10
     misc_feature    152..180
                     /label=O2
     misc_feature    153..153
                     /label=Transcriptional Start Site
     RBS             304..312
                     /label=Native RBS
     promoter        1..314
                     /label=BBa_K1820003
     misc_feature    321..323
                     /label=start codon
     CDS             324..1034
                     /label=sfGFP
     misc_feature    1034..1037
                     /label=stop codon
     misc_feature    321..1037
                     /label=BBa_K1820004
     stem_loop       1057..1100
                     /label=stem_loop
     misc_feature    1046..1125
                     /label=BBa_B0010
     terminator      1046..1125
                     /label=BBa_B0010
     stem_loop       1141..1160
                     /label=T7 TE
     misc_feature    1167..1167
                     /label=stop
     polyA_site      1161..1174
                     /label=polya
     misc_feature    1134..1174
                     /label=BBa_B0012
     terminator      1134..1174
                     /label=BBa_B0012
     terminator      1046..1174
                     /label=BBa_B0015
     misc_feature    321..1174
                     /label=BBa_K1820006
ORIGIN
        1 cctgtcttaa tcctcggtcg ttcattattt tctttaatct catttgagag ttttcttttt
       61 tcatgaactt attataaaaa catttataaa aaagttcaaa ctttttatac tttttccttg
      121 acaattattt tccaaatgat ataattaaat cataaagttc aaaacatttg aactttatat
      181 caaacaagcg aacaatcatg gagcattcag tacggcagac ggaacaggct caaatgacgg
      241 tacacgacgt atccaccgcg acgtaagtag caagtttggc aaataaaaag ccccagaggg
      301 gcagaaagga tgtatactag atgtcaaaag gagaagagct gttcacaggt gttgtgccga
      361 ttctcgttga gcttgacgga gatgtaaacg gacacaaatt ctctgttcgc ggtgaaggtg
      421 aaggagatgc aacaaacggc aagctgacat tgaagtttat ttgcacaact ggaaagctgc
      481 cggttccttg gccgacactt gtaacgacgc tgacttacgg cgttcaatgc ttctctcgtt
      541 atccagacca catgaaacgc catgatttct tcaaatctgc aatgcctgaa ggctacgttc
      601 aagagcgtac gatcagcttc aaagatgacg gaacgtacaa aacaagagca gaagtgaagt
      661 ttgaaggtga cacacttgtg aaccgcattg aattgaaagg cattgatttc aaagaagatg
      721 gaaacatcct tggacacaaa cttgaataca acttcaacag ccacaacgta tacatcactg
      781 ctgacaaaca aaaaaacggc atcaaagcaa acttcaaaat ccgtcataac gtagaggacg
      841 gttctgttca gcttgctgat cattatcagc aaaatacacc gatcggtgac ggcccggttc
      901 ttcttcctga taaccattat ttatcaactc aaagcgtatt atcaaaagac ccaaatgaaa
      961 agcgtgacca catggtgctg cttgaatttg tgacagctgc tggtatcact cacggcatgg
     1021 atgagcttta taagtaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1081 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1141 ggctcacctt cgggtgggcc tttctgcgtt tata
//