BBa_K1820006 1 BBa_K1820006 sfGFP(Bs) + terminator 2015-09-15T11:00:00Z 2015-09-18T05:59:15Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen component2474479 1 BBa_K1820004 component2474491 1 BBa_B0015 annotation2474479 1 BBa_K1820004 range2474479 1 1 717 annotation2474491 1 BBa_B0015 range2474491 1 726 854 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1820026 1 BBa_K1820026 p2001-native RBS-sfGFP-B0015 2015-09-15T11:00:00Z 2015-09-16T03:36:49Z Add Add false false _2245_ 25685 25685 9 false Add false Chad Lawrence Nielsen component2462231 1 BBa_K1820006 component2462222 1 BBa_K1820003 annotation2462231 1 BBa_K1820006 range2462231 1 321 1174 annotation2462222 1 BBa_K1820003 range2462222 1 1 314 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1820003 1 BBa_K1820003 P335-&#934;31phage genetic switch-divergent promoter + native RBS 2015-09-15T11:00:00Z 2015-09-17T10:20:40Z Add Add false false _2245_ 25667 25667 9 false Add false Thomas Overbeck annotation2466356 1 Rightward -10 range2466356 1 140 145 annotation2466355 1 Rightward -35 range2466355 1 118 123 annotation2466354 1 Rightward Promoter range2466354 1 118 145 annotation2466359 1 Native RBS range2466359 1 304 312 annotation2466350 1 Leftward Promoter range2466350 1 71 99 annotation2466351 1 Leftward -10 range2466351 1 71 76 annotation2466352 1 O1 range2466352 1 88 115 annotation2466353 1 Leftward -35 range2466353 1 94 99 annotation2466358 1 Transcriptional Start Site range2466358 1 153 153 annotation2466357 1 O2 range2466357 1 152 180 BBa_K1820004 1 BBa_K1820004 sfGFP(Bs) 2015-09-15T11:00:00Z 2015-09-18T08:18:27Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen annotation2474464 1 sfGFP range2474464 1 4 714 annotation2475184 1 stop codon range2475184 1 714 717 annotation2474463 1 start codon range2474463 1 1 3 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1820006_sequence 1 atgtcaaaaggagaagagctgttcacaggtgttgtgccgattctcgttgagcttgacggagatgtaaacggacacaaattctctgttcgcggtgaaggtgaaggagatgcaacaaacggcaagctgacattgaagtttatttgcacaactggaaagctgccggttccttggccgacacttgtaacgacgctgacttacggcgttcaatgcttctctcgttatccagaccacatgaaacgccatgatttcttcaaatctgcaatgcctgaaggctacgttcaagagcgtacgatcagcttcaaagatgacggaacgtacaaaacaagagcagaagtgaagtttgaaggtgacacacttgtgaaccgcattgaattgaaaggcattgatttcaaagaagatggaaacatccttggacacaaacttgaatacaacttcaacagccacaacgtatacatcactgctgacaaacaaaaaaacggcatcaaagcaaacttcaaaatccgtcataacgtagaggacggttctgttcagcttgctgatcattatcagcaaaatacaccgatcggtgacggcccggttcttcttcctgataaccattatttatcaactcaaagcgtattatcaaaagacccaaatgaaaagcgtgaccacatggtgctgcttgaatttgtgacagctgctggtatcactcacggcatggatgagctttataagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1820003_sequence 1 cctgtcttaatcctcggtcgttcattattttctttaatctcatttgagagttttcttttttcatgaacttattataaaaacatttataaaaaagttcaaactttttatactttttccttgacaattattttccaaatgatataattaaatcataaagttcaaaacatttgaactttatatcaaacaagcgaacaatcatggagcattcagtacggcagacggaacaggctcaaatgacggtacacgacgtatccaccgcgacgtaagtagcaagtttggcaaataaaaagccccagaggggcagaaaggatgta BBa_K1820004_sequence 1 atgtcaaaaggagaagagctgttcacaggtgttgtgccgattctcgttgagcttgacggagatgtaaacggacacaaattctctgttcgcggtgaaggtgaaggagatgcaacaaacggcaagctgacattgaagtttatttgcacaactggaaagctgccggttccttggccgacacttgtaacgacgctgacttacggcgttcaatgcttctctcgttatccagaccacatgaaacgccatgatttcttcaaatctgcaatgcctgaaggctacgttcaagagcgtacgatcagcttcaaagatgacggaacgtacaaaacaagagcagaagtgaagtttgaaggtgacacacttgtgaaccgcattgaattgaaaggcattgatttcaaagaagatggaaacatccttggacacaaacttgaatacaacttcaacagccacaacgtatacatcactgctgacaaacaaaaaaacggcatcaaagcaaacttcaaaatccgtcataacgtagaggacggttctgttcagcttgctgatcattatcagcaaaatacaccgatcggtgacggcccggttcttcttcctgataaccattatttatcaactcaaagcgtattatcaaaagacccaaatgaaaagcgtgaccacatggtgctgcttgaatttgtgacagctgctggtatcactcacggcatggatgagctttataagtaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1820026_sequence 1 cctgtcttaatcctcggtcgttcattattttctttaatctcatttgagagttttcttttttcatgaacttattataaaaacatttataaaaaagttcaaactttttatactttttccttgacaattattttccaaatgatataattaaatcataaagttcaaaacatttgaactttatatcaaacaagcgaacaatcatggagcattcagtacggcagacggaacaggctcaaatgacggtacacgacgtatccaccgcgacgtaagtagcaagtttggcaaataaaaagccccagaggggcagaaaggatgtatactagatgtcaaaaggagaagagctgttcacaggtgttgtgccgattctcgttgagcttgacggagatgtaaacggacacaaattctctgttcgcggtgaaggtgaaggagatgcaacaaacggcaagctgacattgaagtttatttgcacaactggaaagctgccggttccttggccgacacttgtaacgacgctgacttacggcgttcaatgcttctctcgttatccagaccacatgaaacgccatgatttcttcaaatctgcaatgcctgaaggctacgttcaagagcgtacgatcagcttcaaagatgacggaacgtacaaaacaagagcagaagtgaagtttgaaggtgacacacttgtgaaccgcattgaattgaaaggcattgatttcaaagaagatggaaacatccttggacacaaacttgaatacaacttcaacagccacaacgtatacatcactgctgacaaacaaaaaaacggcatcaaagcaaacttcaaaatccgtcataacgtagaggacggttctgttcagcttgctgatcattatcagcaaaatacaccgatcggtgacggcccggttcttcttcctgataaccattatttatcaactcaaagcgtattatcaaaagacccaaatgaaaagcgtgaccacatggtgctgcttgaatttgtgacagctgctggtatcactcacggcatggatgagctttataagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z