LOCUS       BBa_K1820027            1168 bp    DNA     linear       11-Jun-2024
DEFINITION  p2001-native RBS-mCherry-B0015
ACCESSION   BBa_K1820027
VERSION     BBa_K1820027.1  
FEATURES             Location/Qualifiers
     promoter        71..99
                     /label=Leftward Promoter
     misc_feature    71..76
                     /label=Leftward -10
     misc_feature    88..115
                     /label=O1
     misc_feature    94..99
                     /label=Leftward -35
     promoter        118..145
                     /label=Rightward Promoter
     misc_feature    118..123
                     /label=Rightward -35
     misc_feature    140..145
                     /label=Rightward -10
     misc_feature    152..180
                     /label=O2
     misc_feature    153..153
                     /label=Transcriptional Start Site
     RBS             304..312
                     /label=Native RBS
     promoter        1..314
                     /label=BBa_K1820003
     misc_feature    321..323
                     /label=start codon
     CDS             324..1028
                     /label=mCherry
     misc_feature    1028..1031
                     /label=stop codon
     misc_feature    321..1031
                     /label=BBa_K1820005
     stem_loop       1051..1094
                     /label=stem_loop
     misc_feature    1040..1119
                     /label=BBa_B0010
     terminator      1040..1119
                     /label=BBa_B0010
     stem_loop       1135..1154
                     /label=T7 TE
     misc_feature    1161..1161
                     /label=stop
     polyA_site      1155..1168
                     /label=polya
     misc_feature    1128..1168
                     /label=BBa_B0012
     terminator      1128..1168
                     /label=BBa_B0012
     terminator      1040..1168
                     /label=BBa_B0015
     misc_feature    321..1168
                     /label=BBa_K1820007
ORIGIN
        1 cctgtcttaa tcctcggtcg ttcattattt tctttaatct catttgagag ttttcttttt
       61 tcatgaactt attataaaaa catttataaa aaagttcaaa ctttttatac tttttccttg
      121 acaattattt tccaaatgat ataattaaat cataaagttc aaaacatttg aactttatat
      181 caaacaagcg aacaatcatg gagcattcag tacggcagac ggaacaggct caaatgacgg
      241 tacacgacgt atccaccgcg acgtaagtag caagtttggc aaataaaaag ccccagaggg
      301 gcagaaagga tgtatactag atggtttcta agggtgaaga agataatatg gctattatta
      361 aggaatttat gcgttttaag gtacatatgg aaggtagtgt taatggtcat gaatttgaaa
      421 ttgaaggtga aggagaaggt cggccatatg aaggtactca aactgctaag ttaaaggtta
      481 ctaaaggtgg accattacct tttgcatggg atattctttc accacaattt atgtacggat
      541 ctaaggctta tgttaagcac ccagcagata ttcctgatta ccttaaactt agttttcctg
      601 aaggttttaa gtgggaacga gttatgaatt ttgaagatgg tggagttgta actgtaacac
      661 aagatagttc attacaagat ggagaattta tttacaaggt taagttacgt ggaactaatt
      721 ttccatcaga tggtcctgta atgcaaaaga aaactatggg ttgggaagct agtagtgaac
      781 gtatgtaccc agaagatgga gcattaaagg gtgaaattaa gcaacggctt aaacttaagg
      841 atggtggaca ttacgatgct gaagttaaga ctacatacaa ggcaaaaaag ccagttcaat
      901 tacctggtgc ttacaatgta aatattaagt tagatattac ttctcacaat gaagattaca
      961 caattgttga acaatacgaa cgggcagaag gtcggcactc tactggagga atggatgaac
     1021 tttacaaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1081 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1141 ccttcgggtg ggcctttctg cgtttata
//