##gff-version 3
##sequence-region BBa_K1820027 1 1168
BBa_K1820027	.	promoter	1	314	.	+	0	ID=BBa_K1820003;Name=BBa_K1820003
BBa_K1820027	.	promoter	71	99	.	+	0	ID=annotation2466350;Name=Leftward Promoter;Parent=BBa_K1820003
BBa_K1820027	.	non_covalent_binding_site	71	76	.	+	0	ID=annotation2466351;Name=Leftward -10;Parent=BBa_K1820003
BBa_K1820027	.	non_covalent_binding_site	118	123	.	+	0	ID=annotation2466355;Name=Rightward -35;Parent=BBa_K1820003
BBa_K1820027	.	non_covalent_binding_site	94	99	.	+	0	ID=annotation2466353;Name=Leftward -35;Parent=BBa_K1820003
BBa_K1820027	.	promoter	118	145	.	+	0	ID=annotation2466354;Name=Rightward Promoter;Parent=BBa_K1820003
BBa_K1820027	.	non_covalent_binding_site	140	145	.	+	0	ID=annotation2466356;Name=Rightward -10;Parent=BBa_K1820003
BBa_K1820027	.	operator	88	115	.	+	0	ID=annotation2466352;Name=O1;Parent=BBa_K1820003
BBa_K1820027	.	ribosome_entry_site	304	312	.	+	0	ID=annotation2466359;Name=Native RBS;Parent=BBa_K1820003
BBa_K1820027	.	sequence_feature	153	153	.	+	0	ID=annotation2466358;Name=Transcriptional Start Site;Parent=BBa_K1820003
BBa_K1820027	.	operator	152	180	.	+	0	ID=annotation2466357;Name=O2;Parent=BBa_K1820003
BBa_K1820027	.	engineered_region	321	1168	.	+	0	ID=BBa_K1820007;Name=BBa_K1820007
BBa_K1820027	.	engineered_region	321	1031	.	+	0	ID=BBa_K1820005;Name=BBa_K1820005;Parent=BBa_K1820007
BBa_K1820027	.	start_codon	1028	1031	.	+	0	ID=annotation2475183;Name=stop codon;Parent=BBa_K1820005
BBa_K1820027	.	CDS	324	1028	.	+	0	ID=annotation2474461;Name=mCherry;Parent=BBa_K1820005
BBa_K1820027	.	start_codon	321	323	.	+	0	ID=annotation2474460;Name=start codon;Parent=BBa_K1820005
BBa_K1820027	.	terminator	1040	1168	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1820007
BBa_K1820027	.	terminator	1040	1119	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1820027	.	stem_loop	1051	1094	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1820027	.	engineered_region	1040	1119	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1820027	.	terminator	1128	1168	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1820027	.	engineered_region	1128	1168	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1820027	.	stop_codon	1161	1161	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1820027	.	stem_loop	1135	1154	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1820027	.	polyA_site	1155	1168	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K1820027
cctgtcttaatcctcggtcgttcattattttctttaatctcatttgagagttttcttttttcatgaacttattataaaa
acatttataaaaaagttcaaactttttatactttttccttgacaattattttccaaatgatataattaaatcataaagt
tcaaaacatttgaactttatatcaaacaagcgaacaatcatggagcattcagtacggcagacggaacaggctcaaatga
cggtacacgacgtatccaccgcgacgtaagtagcaagtttggcaaataaaaagccccagaggggcagaaaggatgtata
ctagatggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggt
agtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaa
aggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgt
taagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaatttt
gaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtg
gaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtaccc
agaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaag
actacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcaca
atgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaa
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata