BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1820027 1 BBa_K1820027 p2001-native RBS-mCherry-B0015 2015-09-15T11:00:00Z 2015-09-16T03:38:49Z Add Add false false _2245_ 25685 25685 9 false Add false Chad Lawrence Nielsen component2462243 1 BBa_K1820007 component2462234 1 BBa_K1820003 annotation2462243 1 BBa_K1820007 range2462243 1 321 1168 annotation2462234 1 BBa_K1820003 range2462234 1 1 314 BBa_K1820003 1 BBa_K1820003 P335-&#934;31phage genetic switch-divergent promoter + native RBS 2015-09-15T11:00:00Z 2015-09-17T10:20:40Z Add Add false false _2245_ 25667 25667 9 false Add false Thomas Overbeck annotation2466355 1 Rightward -35 range2466355 1 118 123 annotation2466357 1 O2 range2466357 1 152 180 annotation2466356 1 Rightward -10 range2466356 1 140 145 annotation2466350 1 Leftward Promoter range2466350 1 71 99 annotation2466352 1 O1 range2466352 1 88 115 annotation2466353 1 Leftward -35 range2466353 1 94 99 annotation2466354 1 Rightward Promoter range2466354 1 118 145 annotation2466351 1 Leftward -10 range2466351 1 71 76 annotation2466359 1 Native RBS range2466359 1 304 312 annotation2466358 1 Transcriptional Start Site range2466358 1 153 153 BBa_K1820007 1 BBa_K1820007 mCherry(Lr) + terminator 2015-09-15T11:00:00Z 2015-09-18T06:05:40Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen component2474591 1 BBa_B0015 component2474584 1 BBa_K1820005 annotation2474584 1 BBa_K1820005 range2474584 1 1 711 annotation2474591 1 BBa_B0015 range2474591 1 720 848 BBa_K1820005 1 BBa_K1820005 mCherry(Lr) 2015-09-15T11:00:00Z 2015-09-18T08:17:16Z Add Add false false _2245_ 25686 25686 9 false Add false Alex Torgesen annotation2474461 1 mCherry range2474461 1 4 708 annotation2475183 1 stop codon range2475183 1 708 711 annotation2474460 1 start codon range2474460 1 1 3 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1820003_sequence 1 cctgtcttaatcctcggtcgttcattattttctttaatctcatttgagagttttcttttttcatgaacttattataaaaacatttataaaaaagttcaaactttttatactttttccttgacaattattttccaaatgatataattaaatcataaagttcaaaacatttgaactttatatcaaacaagcgaacaatcatggagcattcagtacggcagacggaacaggctcaaatgacggtacacgacgtatccaccgcgacgtaagtagcaagtttggcaaataaaaagccccagaggggcagaaaggatgta BBa_K1820027_sequence 1 cctgtcttaatcctcggtcgttcattattttctttaatctcatttgagagttttcttttttcatgaacttattataaaaacatttataaaaaagttcaaactttttatactttttccttgacaattattttccaaatgatataattaaatcataaagttcaaaacatttgaactttatatcaaacaagcgaacaatcatggagcattcagtacggcagacggaacaggctcaaatgacggtacacgacgtatccaccgcgacgtaagtagcaagtttggcaaataaaaagccccagaggggcagaaaggatgtatactagatggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1820005_sequence 1 atggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataa BBa_K1820007_sequence 1 atggtttctaagggtgaagaagataatatggctattattaaggaatttatgcgttttaaggtacatatggaaggtagtgttaatggtcatgaatttgaaattgaaggtgaaggagaaggtcggccatatgaaggtactcaaactgctaagttaaaggttactaaaggtggaccattaccttttgcatgggatattctttcaccacaatttatgtacggatctaaggcttatgttaagcacccagcagatattcctgattaccttaaacttagttttcctgaaggttttaagtgggaacgagttatgaattttgaagatggtggagttgtaactgtaacacaagatagttcattacaagatggagaatttatttacaaggttaagttacgtggaactaattttccatcagatggtcctgtaatgcaaaagaaaactatgggttgggaagctagtagtgaacgtatgtacccagaagatggagcattaaagggtgaaattaagcaacggcttaaacttaaggatggtggacattacgatgctgaagttaagactacatacaaggcaaaaaagccagttcaattacctggtgcttacaatgtaaatattaagttagatattacttctcacaatgaagattacacaattgttgaacaatacgaacgggcagaaggtcggcactctactggaggaatggatgaactttacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z