LOCUS       BBa_K182003             2071 bp    DNA     linear       26-Jun-2024
DEFINITION  Intermediate part consisting of CI promoter followed by tetR, LacI 
            and Tet Operator
ACCESSION   BBa_K182003
VERSION     BBa_K182003.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             79..695
                     /label=tetR
     misc_feature    696..729
                     /label=SsrA
     misc_feature    736..760
                     /label=Help:Barcodes
     CDS             76..760
                     /label=BBa_C0040
     misc_feature    58..760
                     /label=BBa_S03518
     misc_feature    1..760
                     /label=BBa_K182002
     misc_feature    773..776
                     /label=conserved
     RBS             769..780
                     /label=BBa_B0034
     CDS             787..1872
                     /label=BBa_I732100
     stem_loop       1892..1935
                     /label=stem_loop
     misc_feature    1881..1960
                     /label=BBa_B0010
     terminator      1881..1960
                     /label=BBa_B0010
     stem_loop       1976..1995
                     /label=T7 TE
     misc_feature    2002..2002
                     /label=stop
     polyA_site      1996..2009
                     /label=polya
     misc_feature    1969..2009
                     /label=BBa_B0012
     terminator      1969..2009
                     /label=BBa_B0012
     misc_feature    2018..2036
                     /label=TetR 1
     misc_feature    2018..2071
                     /label=BBa_R0040
     promoter        2037..2042
                     /label=-35
     misc_feature    2043..2061
                     /label=TetR 2
     promoter        2060..2065
                     /label=-10
     promoter        2018..2071
                     /label=BBa_R0040
     misc_feature    769..2071
                     /label=BBa_K182001
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
      121 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
      181 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
      241 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
      301 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
      361 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
      421 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
      481 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
      541 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
      601 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
      661 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct
      721 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagaa agaggagaaa
      781 tactagatga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag
      841 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg
      901 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc
      961 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa
     1021 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg
     1081 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc
     1141 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct
     1201 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt
     1261 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt
     1321 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg
     1381 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc
     1441 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt
     1501 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc
     1561 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac
     1621 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa
     1681 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg
     1741 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct
     1801 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     1861 gggcagtaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1921 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1981 accttcgggt gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga
     2041 catccctatc agtgatagag atactgagca c
//