LOCUS       BBa_K182100             1661 bp    DNA     linear       22-Dec-2024
DEFINITION  Intermediate Part consisting of Lambda cI with Reporter Gene and 
            double Terminators
ACCESSION   BBa_K182100
VERSION     BBa_K182100.1  
FEATURES             Location/Qualifiers
     CDS             4..711
                     /label=cI lambda
     misc_feature    712..744
                     /label=LVA
     misc_feature    751..775
                     /label=Help:Barcodes
     CDS             1..775
                     /label=BBa_C0051
     RBS             784..798
                     /label=RBS-1\Strong
     RBS             791..795
                     /label=RBS
     misc_feature    784..798
                     /label=BBa_B0030
     RBS             784..798
                     /label=BBa_B0030
     CDS             805..1524
                     /label=GFP protein
     CDS             805..1524
                     /label=BBa_E0040
     stem_loop       1544..1587
                     /label=stem_loop
     misc_feature    1533..1612
                     /label=BBa_B0010
     terminator      1533..1612
                     /label=BBa_B0010
     stem_loop       1628..1647
                     /label=T7 TE
     misc_feature    1654..1654
                     /label=stop
     polyA_site      1648..1661
                     /label=polya
     misc_feature    1621..1661
                     /label=BBa_B0012
     terminator      1621..1661
                     /label=BBa_B0012
     misc_feature    784..1661
                     /label=BBa_E0840
ORIGIN
        1 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa
       61 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag
      121 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct
      181 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca
      241 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt
      301 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga
      361 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct
      421 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc
      481 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc
      541 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt
      601 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt
      661 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgctgcaaac
      721 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta
      781 gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc
      841 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      901 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      961 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
     1021 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
     1081 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
     1141 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     1201 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
     1261 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
     1321 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     1381 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     1441 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     1501 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca
     1561 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1621 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//