LOCUS       BBa_K182101              960 bp    DNA     linear       22-Dec-2024
DEFINITION  pLux-Lac Hybrid with GFP Reporter and Double Terminator
ACCESSION   BBa_K182101
VERSION     BBa_K182101.1  
FEATURES             Location/Qualifiers
     promoter        20..74
                     /label=plac
     misc_feature    20..26
                     /label=-35
     misc_feature    42..48
                     /label=-10
     misc_feature    27..41
                     /label=O1
     misc_feature    49..74
                     /label=O1
     misc_feature    1..19
                     /label=luxR binding site
     promoter        1..74
                     /label=BBa_I751502
     RBS             83..97
                     /label=RBS-1\Strong
     RBS             90..94
                     /label=RBS
     misc_feature    83..97
                     /label=BBa_B0030
     RBS             83..97
                     /label=BBa_B0030
     CDS             104..823
                     /label=GFP protein
     CDS             104..823
                     /label=BBa_E0040
     stem_loop       843..886
                     /label=stem_loop
     misc_feature    832..911
                     /label=BBa_B0010
     terminator      832..911
                     /label=BBa_B0010
     stem_loop       927..946
                     /label=T7 TE
     misc_feature    953..953
                     /label=stop
     polyA_site      947..960
                     /label=polya
     misc_feature    920..960
                     /label=BBa_B0012
     terminator      920..960
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacttgtga gcggataaca atatagtgtg tggaattgtg
       61 agcggataac aatttactag agattaaaga ggagaaatac tagatgcgta aaggagaaga
      121 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      181 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      241 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
      301 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
      361 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
      421 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      481 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
      541 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
      601 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
      661 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
      721 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
      781 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc
      841 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      901 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//